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Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum

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Abstract

A novel transglycosylation reaction from sucrose to l-ascorbic acid by a recombinant sucrose phosphorylase from Bifidobacterium longum was used to produce a stable l-ascorbic acid derivative. The major product was detected by HPLC, and confirmed to be 2-O-α-d-glucopyranosyl-l-ascorbic acid by LC-MS/MS analysis.

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Acknowledgement

This research was supported by Kyonggi University Overseas Research Grant 2004.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Kyonggi University, Suwon, 443-760, South Korea

    Taeyeon Kwon & Jong-Hoon Lee

  2. Nong Shim Co., LTD., Kunpo, 435-713, South Korea

    Cheong Tae Kim

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  1. Taeyeon Kwon
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  2. Cheong Tae Kim
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  3. Jong-Hoon Lee
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Correspondence to Jong-Hoon Lee.

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Kwon, T., Kim, C. & Lee, JH. Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum . Biotechnol Lett 29, 611–615 (2007). https://doi.org/10.1007/s10529-006-9285-2

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  • DOI: https://doi.org/10.1007/s10529-006-9285-2

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