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Production of d-tagatose at high temperatures using immobilized Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana

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Abstract

Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t 1/2 = 43 days at 70°C) in a continuous recycling mode at 70°C produced 49 and 38 g d-tagatose/l from 180 and 90 g d-galactose/l, respectively, within 12 h.

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References

  • Adachi S, Sugawara H (1963) Separation of ketose and aldose by chromatography on an ion-exchange column. Arch Biochem Biophys 100:468–471

    Article  PubMed  CAS  Google Scholar 

  • Beadle JR, Saunders JP, Wajda TJ (1992) Process for manufacturing tagatose. U.S. Patent 5,078,796

  • Busto MD (1998) An experiment illustrating the effect of immobilization on enzyme properties. Biochem Educ 26:304–308

    Article  CAS  Google Scholar 

  • Cheetham PSJ, Wootton AN (1992) Bioconversion of d-galactose into d-tagatose. Enzyme Microb Technol 15:105–108

    Article  Google Scholar 

  • Dische Z, Borefreund E (1951) A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem 192:583–587

    PubMed  CAS  Google Scholar 

  • Ibrahim OO, Spradlin JE (2000) Process for manufacturing d-tagatose. U.S. Patent 6,057,135

  • Jørgensen F, Hansen OC, Stougaard P (2004) Enzymatic conversion of d-galactose to d-tagatose:heterologous expression and characterization of a thermostable l-arabinose isomerase from Thermoanaerobacter mathranii. Appl Microbiol Biotechnol 64:816–822

    Article  PubMed  Google Scholar 

  • Kim BC, Lee YH, Lee HS, Lee DW, Choe EA, Pyun YR (2002) Cloning, expression and characterization of l-arabinose isomerase from Thermotoga neapolitana: bioconversion of d-galactose to d-tagatose using the enzyme. FEMS Microbiol Lett 212:121–126

    PubMed  CAS  Google Scholar 

  • Lee DW, Jang HJ, Choe EA, Kim BC, Lee SJ, Kim SB, Hong YH, Pyun YR (2004) Characterization of a thermostable l-arabinose isomerase (d-galactose) isomerase from the hyperthermophilic eubacterium Thermotoga maritima. Appl Environ Microbiol 70:1397–1404

    Article  PubMed  CAS  Google Scholar 

  • Lee DW, Choe EA, Kim SB, Eom SH, Hong YH, Lee SJ, Lee HS, Lee DY, Pyun YR (2005a) Distinct metal dependence for catalytic and structural functions in the l-arabinose isomerases from the mesophilic Bacillus halodurans and the thermophilic Geobacillus stearothermophilius. Arch Biochem Biophys 434:333–343

    Article  CAS  Google Scholar 

  • Lee DW, Hong YH, Choe EA, Lee SJ, Kim SB, Lee HS, Oh JW, Shin HH, Pyun YR (2005b) A thermodynamic study of mesophilic, thermophilic, and hyperthermophilic l-arabinose isomerases: the effects of divalent metal ions on protein stability at elevated temperatures. FEBS Lett 579:1261–1266

    Article  CAS  Google Scholar 

  • Lernia ID, Schiraldi C, Generoso M, Rosa MD (2002) Trehalose production at high temperature exploiting an immobilized cell reactor. Extremophiles 6:341–347

    Article  PubMed  Google Scholar 

  • Levin GV (2002) Tagatose, the new GRAS sweetener and health product. J Med Food 5:23–36

    Article  PubMed  CAS  Google Scholar 

  • Nabors LO (2002) Sweet choices:sugar replacements for foods and beverages. Food Technol 56:28–45

    Google Scholar 

  • Ortega N, Busto MD, Perez-Mateos M (1998) Stabilization of β-glucosidase entrapped in alginate and polyacrylamide gels towards thermal and proteolytic deactivation. J Chem Technol Biotechnol 73:7–12

    Article  CAS  Google Scholar 

  • Ryu SA, Kim CS, Kim HJ, Baek DH, Oh DK (2003) Continuous d-tagatose production by immobilized thermostable l-arabinose isomerase in a packed-bed bioreactor. Biotechnol Prog 19:1643–1647

    Article  PubMed  CAS  Google Scholar 

  • Shaw CJ, Huang A, Zhang X (2003) Quantitation and test of enantiomeric purity of the l-ketohexoses by liquid chromatography with dual refractive index and laser-based chiroptical detection. J Chromatogr A 987:439–443

    Article  PubMed  CAS  Google Scholar 

  • Tewari YB, Goldberg RN (1985) An investigation of the equilibria between aqueous ribose, ribulose, and arabinose. Biophys Chem 22:197–204

    Article  PubMed  CAS  Google Scholar 

  • Tewari YB, Steckler DK, Goldberg RN (1985) Thermodynamics of the conversion of aqueous xylose to xylulose. Biophys Chem 22:181–185

    Article  PubMed  CAS  Google Scholar 

  • Zehner LR (1988) d-Tagatose as a low-calorie carbohydrate sweetener and bulking agent. U.S. Patent 4,786,722

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Acknowledgements

This work was supported by grant 2001-2-0109 from the Korea Science and Engineering Foundation and grant AIC-08-02 from the Ministry of Commerce, Industry and Energy, Korea.

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Authors and Affiliations

  1. Department of Biotechnology, Yonsei University, Seoul, 120-749, Korea

    Young-Ho Hong, Sang-Jae Lee, Chan-Ick Cheigh & Yu-Ryang Pyun

  2. Department of Biology, University of Pennsylvania, Philadelphia, PA, 19104, USA

    Dong-Woo Lee

  3. Department of Human Genetics, Emory University, Atlanta, GA, 30322, USA

    Eun-Ah Choe

  4. R&D Center of Sugar Flour & Edible Oil Planning, CJ Corp., Seoul, 100-802, Korea

    Seong-Bo Kim

  5. Department of Food Science and Technology, Ohio State University, Columbus, OH, 43210, USA

    Yoon-Hee Lee

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  1. Young-Ho Hong
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  2. Dong-Woo Lee
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  3. Sang-Jae Lee
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  4. Eun-Ah Choe
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Correspondence to Yu-Ryang Pyun.

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Hong, YH., Lee, DW., Lee, SJ. et al. Production of d-tagatose at high temperatures using immobilized Escherichia coli cells expressing l-arabinose isomerase from Thermotoga neapolitana . Biotechnol Lett 29, 569–574 (2007). https://doi.org/10.1007/s10529-006-9277-2

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  • DOI: https://doi.org/10.1007/s10529-006-9277-2

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