Abstract
An expression vector constructed from genes of Pichia pastoris was applied for heterologous gene expression in Saccharomyces cerevisiae. Recombinant hepatitis B surface antigen was synthesized by cloning hepatitis B virus ‘S’ gene under the control of glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter of Pichia pastoris in Saccharomyces cerevisiae. Hepatitis B surface antigen was constitutively expressed, was stable and exhibited ∼20–22 nm particle formation. Stability and absence of toxicity to the host with the expression vector indicates the expression system can be applied for large-scale production.
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R.N.V. is a Council of Scientific and Industrial Research (CSIR) fellow.
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Vellanki, R.N., Komaravelli, N., Tatineni, R. et al. Expression of hepatitis B surface antigen in Saccharomyces cerevisiae utilizing glyceraldeyhyde-3-phosphate dehydrogenase promoter of Pichia pastoris . Biotechnol Lett 29, 313–318 (2007). https://doi.org/10.1007/s10529-006-9242-0
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DOI: https://doi.org/10.1007/s10529-006-9242-0