Abstract
In the baculovirus expression vector system (BEVS), secretory green fluorescent protein (sGFP) transcripts were expressed from day 2 to day 5 post-infection (p.i.), while transcripts of the endoplasmic reticulum (ER) molecular chaperone Bombyx mori protein disulfide isomerase (bPDI) were measurable in mock cells and in cells at day 1 p.i. The GFP was expressed from day 3 to day 5 p.i. whereas the levels of two ER chaperone proteins, bPDI and calnexin, decreased from day 3 p.i. and were not detected from day 4 p.i. These findings suggest that the rate-limited expression of ER molecular chaperones is strongly associated with the maximal expression of exogenous proteins in BEVS.
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Revisions requested 13 April 2005; Revisions received 17 May 2005
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Yun, EY., Goo, TW., Kim, SW. et al. Changes in cellular secretory processing during baculovirus infection. Biotechnol Lett 27, 1041–1045 (2005). https://doi.org/10.1007/s10529-005-8108-1
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DOI: https://doi.org/10.1007/s10529-005-8108-1