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Stable expression and visualization of Mat-8 (FXYD-3) tagged with a fluorescent protein in Chinese Hamster Ovary (CHO)-K1 cells

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Abstract

A type I transmembrane protein, Mat-8 (FXYD-3), was tagged with fluorescent protein, Discosoma red fluorescent protein, at the carboxyl terminal cytoplasmic tail, and stably expressed in Chinese Hamster Ovary (CHO)-K1 cells. The fluorescence signal was distributed in intracellular membranes, being not only detected around the nuclear envelope but also partly overlapping with an endoplasmic reticulum marker. Subcellular fractionation by density gradient centrifugation supported this partial overlapping. The spherical structures observed were not colocalized with markers for lysosomes, endosomes, and Golgi bodies, suggesting that Mat-8 is distributed in a distinct endoplasmic reticulum region and the nuclear envelope after synthesis on membrane-bound ribosomes.

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Authors and Affiliations

  1. Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, 565-0871, Suita, Osaka, Japan

    Junko Arimochi, Ayako Kobayashi & Masatomo Maeda

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  1. Junko Arimochi
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  2. Ayako Kobayashi
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  3. Masatomo Maeda
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Correspondence to Masatomo Maeda.

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Revisions requested 15 April 2005; Revisions received 11 May 2005

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Arimochi, J., Kobayashi, A. & Maeda, M. Stable expression and visualization of Mat-8 (FXYD-3) tagged with a fluorescent protein in Chinese Hamster Ovary (CHO)-K1 cells. Biotechnol Lett 27, 1017–1024 (2005). https://doi.org/10.1007/s10529-005-7870-4

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  • DOI: https://doi.org/10.1007/s10529-005-7870-4

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