Abstract
Opposite to the established view that collagen is an extracellular substratum for only dispersed hepatocyte culture, hepatocyte spheroids were directly formed within hollow fibers by addition of moderate concentrations of soluble collagen. Morphologically, these spheroids indicated a close relationship with their in vivo structure of liver. The albumin and urea synthetic profiles confirmed that those spheroids maintained liver-specific functions for at least 8 days. Spheroid formation by addition of collagen not only presents a potential methodology for clinical use of spheroids in bioartificial liver device but also indicates a likely function of collagen for self-assembly of primary cells in tissue engineering.
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Received 21 September 2005; Revisions requested 5 October 2005; Revisions received 25 November 2005; Accepted 25 November 2005
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Meng, Q., Wu, D., Zhang, G. et al. Direct Self-assembly of Hepatocytes Spheroids within Hollow Fibers in Presence of Collagen. Biotechnol Lett 28, 279–284 (2006). https://doi.org/10.1007/s10529-005-5531-2
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DOI: https://doi.org/10.1007/s10529-005-5531-2