Skip to main content

Advertisement

SpringerLink
Log in

Apoptosis Induction in Human Lymphoma and Leukemia Cell Lines by Transfection via Dendrosomes Carrying Wild-Type p53 cDNA

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The efficiency of dendrosomes (novel dendritic spheroidal nanoparticle gene porters) were assessed in transferring wild-type p53 cDNA into two human acute lymphoblastic leukemia cell lines (MOLT-4 and CCRF-CEM) derived from T cells and erythroleukemic cell line K562. Flow cytometric studies showed a 65% and 45% enhancement in apoptosis and necrosis of K562 and CCRF-CEM cells transfected with complex of dendrosomes and wild-type p53 cDNA in comparison to controls. The cytotoxicity studies on T lymphoma cells revealed that dendrosomes have a low cytotoxicity in comparison to lipofectin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • SJ Baker S Markowitz ER Fearon (1990) ArticleTitleSuppression of human colorectal carcinoma cell growth by wild-type p53 Science 249 912–915 Occurrence Handle2144057 Occurrence Handle1:STN:280:By%2BA28vktV0%3D

    PubMed  CAS  Google Scholar 

  • T Boulikas (1997) ArticleTitleGene therapy of prostate cancer: p53, suicidal genes, and other targets Anticancer Res. 17 1471–1505 Occurrence Handle9179186 Occurrence Handle1:CAS:528:DyaK2sXktVCmsbo%3D

    PubMed  CAS  Google Scholar 

  • P Buttgereit F Schakowski A Marten K Brand S Renoth (2001) ArticleTitleEffects of adenoviral wild-type p53 gene transfer in p53-mutated lymphoma cells Cancer Gene Ther. 8 430–439 Occurrence Handle11498763 Occurrence Handle10.1038/sj.cgt.7700323 Occurrence Handle1:CAS:528:DC%2BD3MXlslKksbs%3D

    Article  PubMed  CAS  Google Scholar 

  • P Buttgereit S Weineck G Ropke A Marten K Brand (2000) ArticleTitleEfficient gene transfer into lymphoma cells using adenoviral vectors combined with lipofectin Cancer Gene Ther. 7 1145–1155 Occurrence Handle10975675 Occurrence Handle10.1038/sj.cgt.7700209 Occurrence Handle1:CAS:528:DC%2BD3cXmtFyiu7o%3D

    Article  PubMed  CAS  Google Scholar 

  • J Chen J Lin AJ Levine (1995) ArticleTitleRegulation of transcription functions of the p53 tumor suppressor by the mdm-2 oncogene Mol. Med. 1 142–152 Occurrence Handle8529093 Occurrence Handle1:CAS:528:DyaK2MXlt1egsL0%3D

    PubMed  CAS  Google Scholar 

  • C Cinti PP Claudio AD Luca M Cuccurese CM Howard M D’Esposito MG Paggi DL Sala L Azzoni TD Halazonetis A Giordano NM Maraldi (2000) ArticleTitleSerine37 mutation associated with two missense mutations at highly conserved regions of p53 affect pro-apoptotic genes expression in a T-lymphoblastoid drug resistant cell line Oncogene 19 5098–5105 Occurrence Handle11042698 Occurrence Handle10.1038/sj.onc.1203848 Occurrence Handle1:CAS:528:DC%2BD3cXotV2lt7s%3D

    Article  PubMed  CAS  Google Scholar 

  • SE Kern JA Pietenpol S Thiagalingam A Seymour KW Kinzler B Vogelstein (1992) ArticleTitleOncogenic forms of p53 inhibit p53-regulated gene expression Science 256 826–830

    Google Scholar 

  • AJ Levine (1997) ArticleTitleP53 the cellular gate keeper for growth and division Cell 88 323–331 Occurrence Handle9039259 Occurrence Handle10.1016/S0092-8674(00)81871-1 Occurrence Handle1:CAS:528:DyaK2sXhtFahtLg%3D

    Article  PubMed  CAS  Google Scholar 

  • K Maruyama O Ishida T Takizawa K Moribe (1999) ArticleTitlePossibility of active targeting to tumor tissues with liposomes Adv. Drug Deliv. Rev. 40 89–102 Occurrence Handle10837782 Occurrence Handle10.1016/S0169-409X(99)00042-3 Occurrence Handle1:CAS:528:DyaK1MXmsFajsLc%3D

    Article  PubMed  CAS  Google Scholar 

  • H Nakano M Kohara K Shinohara (2001) ArticleTitleEvaluation of the relative contribution of p53-mediated pathway in X-ray-induced apoptosis in human leukemic MOLT-4 cells by Transfection with a mutant p53 gene at different expression levels Cell Tissue Res. 306 101–106 Occurrence Handle11683171 Occurrence Handle10.1007/s004410100438 Occurrence Handle1:CAS:528:DC%2BD3MXmvVegsr0%3D

    Article  PubMed  CAS  Google Scholar 

  • N Rafki FL Roger L Devry C Trentesaux J Dufer (1997) ArticleTitlep53 protein expression in human multidrug-resistant CEM lymphoblasts Leuk. Res. 21 147–152 Occurrence Handle9112432 Occurrence Handle10.1016/S0145-2126(96)00086-0 Occurrence Handle1:CAS:528:DyaK2sXis1WnsLk%3D

    Article  PubMed  CAS  Google Scholar 

  • K Roemer (1999) ArticleTitleMutant p53: Gain-of-function oncoproteins and wild-type p53 inactivators Biol. Chem. 380 879–887 Occurrence Handle10494837 Occurrence Handle10.1515/BC.1999.108 Occurrence Handle1:CAS:528:DyaK1MXls1Kksro%3D

    Article  PubMed  CAS  Google Scholar 

  • MN Sabolouki M Sadeghizadeh MM Yaghoobi A Karami T Lohrasbi (2000) ArticleTitleDendrosomes: A novel family of vehicles for transfection and therapy J. Chem. Tech. Biotech. 75 919–922

    Google Scholar 

  • Y. Y Yin Terauchi GG Solomon S Aizawa PN Rangavajan Y Yazaki T Kadowak JC Barrett (1998) ArticleTitleInvolvement of p85 in p53-dependent apoptosis response to oxidative stress Nature 391 707–710

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Tehran University, P.O. Box 13145-1384, Tehran, Iran

    Mohammad Massumi, Abed Ali Ziaee & Mohammad Nabi Sarbolouki

  2. Department of Stem Cells, Royan Institute, P.O. Box 19395–4644, Tehran, Iran

    Mohammad Massumi

Authors
  1. Mohammad Massumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Abed Ali Ziaee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohammad Nabi Sarbolouki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohammad Massumi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Massumi, M., Ziaee, A.A. & Sarbolouki, M.N. Apoptosis Induction in Human Lymphoma and Leukemia Cell Lines by Transfection via Dendrosomes Carrying Wild-Type p53 cDNA. Biotechnol Lett 28, 61–66 (2006). https://doi.org/10.1007/s10529-005-4689-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-005-4689-y

Keywords

Search

Navigation