Abstract
Growth and the expression of the anti-ErbB2 scFv-Fc-IL2 fusion protein in Chinese hamster ovary (CHO) cells were in association at 37 °C. The expression of the fusion protein was no more than 25 μg/ml. At 30 °C the cell growth was arrested but the cells continued to produce the fusion protein up to 60–80 μg/ml. About 50% of CHO cells were rapidly blocked in G2/M phase after the temperature was shifted from 37 to 30 °C. Lowering temperature resulted in cell growth arrest, but maintained cell viability for a longer time and enhanced the production of the antibody-IL-2 fusion protein in CHO cells.
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Shi, M., Xie, Z., Yu, M. et al. Controlled Growth of Chinese Hamster Ovary Cells and High Expression of Antibody-IL-2 Fusion Proteins by Temperature Manipulation. Biotechnol Lett 27, 1879–1884 (2005). https://doi.org/10.1007/s10529-005-3897-9
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DOI: https://doi.org/10.1007/s10529-005-3897-9