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Cloning and characterization of the Cu,Zn superoxide dismutase (SOD1) cDNA from the mole cricket, Gryllotalpa orientalis

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Abstract

A Cu,Zn superoxide dismutase (SOD1) cDNA was cloned from the mole cricket, Gryllotalpa orientalis. The G. orientalis SOD1 (GoSOD1) cDNA contains an open reading frame of 462 bp encoding 154 amino acid polypeptide with a predicted molecular mass of 15.8 kDa and pI of 6.1, and possesses the typical metal binding ligands of six histidines and one aspartic acid common to SOD1s. The deduced amino acid sequence of the GoSOD1 cDNA showed 75% identity to Lasius niger SOD1, 73% to Apis mellifera SOD1, and 70–68% to SOD1 sequences from other insects. Northern blot analysis revealed the presence of GoSOD1 transcripts in all tissues examined. The expression level of GoATX1 mRNA in the fat body was induced when G. orientalis adult was exposed at low (4 °C) and high (37 °C) temperatures, suggesting that the GoSOD1 seems to play a protective role against oxidative stress caused by temperature shock.

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References

  • CJ Borders JE Saunders DM Blech I Fridovich (1985) ArticleTitleEssentially of the active site arginine residue for the normal catalytic activity of Cu,Zn superoxide dismutase Biochem. J 230 771–776 Occurrence Handle1:CAS:528:DyaL2MXlvFagsrg%3D Occurrence Handle4062877

    CAS  PubMed  Google Scholar 

  • P Chary D Dillon AL Schroeder DO Natvig (1994) ArticleTitleSuperoxide dismutase (sod-1) null mutants of Neurospora crassa: oxidative stress sensitivity, spontaneous mutation rate and response to mutagens Genetics 137 723–730 Occurrence Handle1:CAS:528:DyaK2cXmt1Khur8%3D Occurrence Handle8088518

    CAS  PubMed  Google Scholar 

  • S Chaturvedi AJ Hamilton P Hobby G Zhu CV Lowry V Chaturvedi (2001) ArticleTitleMolecular cloning, phylogenetic analysis and three-dimensional modeling of Cu,Zn superoxide dismutase (CnSOD1) from three varieties of Cryptococcus neoformans Gene 268 41–51 Occurrence Handle10.1016/S0378-1119(01)00408-5 Occurrence Handle1:CAS:528:DC%2BD3MXjsFWrsrw%3D Occurrence Handle11368899

    Article  CAS  PubMed  Google Scholar 

  • JD Crapo T Oury C Rabouille JW Slot LY Chang (1992) ArticleTitleCopper, zinc superoxide dismutase is primarily a cytosolic protein in human cells Proc. Natl. Acad. Sci. USA 89 10405–10409 Occurrence Handle1:CAS:528:DyaK3sXhvFyjsQ%3D%3D Occurrence Handle1332049

    CAS  PubMed  Google Scholar 

  • TP Dalton HG Shertzer A Puga (1999) ArticleTitleRegulation of gene expression by reactive oxygen Annu. Rev. Pharmacol. Toxicol. 39 67–101 Occurrence Handle10.1146/annurev.pharmtox.39.1.67 Occurrence Handle1:CAS:528:DyaK1MXjtVejt7w%3D Occurrence Handle10331077

    Article  CAS  PubMed  Google Scholar 

  • JF Davidson B Whyte PH Bissinger RH Schiestl (1996) ArticleTitleOxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae Proc. Natl. Acad. Sci. USA 93 5116–5121 Occurrence Handle10.1073/pnas.93.10.5116 Occurrence Handle1:CAS:528:DyaK28XjtVKlurg%3D Occurrence Handle8643537

    Article  CAS  PubMed  Google Scholar 

  • P Fabrizio L Liou VN Moy A Diaspro JS Valentine EB Gralla VD Longo (2003) ArticleTitleSOD2 functions downstream of Sch9 to extend longevity in yeast Genetics 163 35–46 Occurrence Handle1:CAS:528:DC%2BD3sXhvFCiur4%3D Occurrence Handle12586694

    CAS  PubMed  Google Scholar 

  • I Fridovich (1986) ArticleTitleSuperoxide dismutases Adv. Enzymol. Rel. Areas Mol. Biol. 58 61–97 Occurrence Handle1:CAS:528:DyaL28XltFSktbc%3D

    CAS  Google Scholar 

  • I Fridovich (1995) ArticleTitleSuperoxide radical and superoxide dismutase Ann. Rev. Biochem. 64 97–112 Occurrence Handle10.1146/annurev.bi.64.070195.000525 Occurrence Handle1:CAS:528:DyaK2MXmsl2qsLs%3D Occurrence Handle7574505

    Article  CAS  PubMed  Google Scholar 

  • PM Hariari DJ Fuller EW Gerner (1989) ArticleTitleHeat shock stimulates polyamine oxidation by two distinct mechanisms in mammalian cell cultures Int. J. Radiat. Oncol. Biol. Phys. 16 451–457 Occurrence Handle2921147

    PubMed  Google Scholar 

  • DJ Jamieson SL Rivers DW Stephen (1994) ArticleTitleAnalysis of Saccharomyces cerevisiae proteins induced by peroxide and superoxide stress Microbiology 140 3277–3283 Occurrence Handle1:CAS:528:DyaK2MXivVCgsrc%3D Occurrence Handle7881546

    CAS  PubMed  Google Scholar 

  • Kim I, Lee KS, Hwang JS, Ahn MY, Li J, Sohn HD, Jin BR (2005) Molecular cloning and characterization of a peroxiredoxin gene from the mole cricket, Gryllotalpa orientalis. Comp. Biochem. Physiol. B. In Press

  • I Kim KS Lee BR Jin ES Kim HS Lee MY Ahn HD Sohn KS Ryu (2002) ArticleTitleMolecular cloning of the Sec61p γ subunit homologue gene from the mole cricket, Gryllotalpa orientalis Int. J. Indust. Entomol. 5 73–77

    Google Scholar 

  • KS Lee SR Kim NS Park I Kim PD Kang BH Sohn KH Choi SW Kang YH Je SM Lee HD Sohn BR Jin (2005) ArticleTitleCharacterization of a silkworm thioredoxin peroxidase that is induced by external temperature stimulus and viral infection Insect Biochem. Mol. Biol. 35 73–84 Occurrence Handle10.1016/j.ibmb.2004.09.008 Occurrence Handle1:CAS:528:DC%2BD2cXhtVyktLnK Occurrence Handle15607657

    Article  CAS  PubMed  Google Scholar 

  • S Lindquist (1986) ArticleTitleThe heat-shock response Annu. Rev. Biochem. 55 1151–1191 Occurrence Handle10.1146/annurev.bi.55.070186.005443 Occurrence Handle1:CAS:528:DyaL28XltV2lsrY%3D Occurrence Handle2427013

    Article  CAS  PubMed  Google Scholar 

  • DP Malinowski I Fridovich (1979) ArticleTitleChemical modification of arginine at the active site of the bovine erythrocyte superoxide dismutase Biochemistry 18 5909–5917 Occurrence Handle10.1021/bi00593a023 Occurrence Handle1:STN:280:Bi%2BD1cfjtVM%3D Occurrence Handle518876

    Article  CAS  PubMed  Google Scholar 

  • JM McCord I Fridovich (1969) ArticleTitleThe utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen J. Biol. Chem. 244 6056–6063 Occurrence Handle1:CAS:528:DyaE3cXmsVY%3D Occurrence Handle4981789

    CAS  PubMed  Google Scholar 

  • GK McMaster GG Carmichael (1977) ArticleTitleAnalysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange Proc. Natl. Acad. Sci. USA 74 4835–4838 Occurrence Handle1:CAS:528:DyaE1cXhvVKltA%3D%3D Occurrence Handle73185

    CAS  PubMed  Google Scholar 

  • DW Mount (1996) ArticleTitleReprogramming transcription Nature 383 763–764 Occurrence Handle10.1038/383763a0 Occurrence Handle1:CAS:528:DyaK28XmsF2rsbc%3D Occurrence Handle8892996

    Article  CAS  PubMed  Google Scholar 

  • WC Orr RS Sohol (1994) ArticleTitleExtension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster Science 263 1128–1130 Occurrence Handle1:CAS:528:DyaK2cXhvVOlsr4%3D Occurrence Handle8108730

    CAS  PubMed  Google Scholar 

  • JD Parker KM Parker BH Sohol RS Sohol L Keller (2004) ArticleTitleDecreased expression of Cu-Zn superoxide dismutase 1 in ants with extreme lifespan Proc. Natl. Acad. Sci. USA 101 3486–4389 Occurrence Handle10.1073/pnas.0400222101 Occurrence Handle1:CAS:528:DC%2BD2cXisFWmsb0%3D Occurrence Handle14993600

    Article  CAS  PubMed  Google Scholar 

  • MW Parker F Bossa D Barra WH Bannister JV Bannister (1986) Structural and evolutionary relationships between the eukaryotic superoxide dismutase G Rotilio (Eds) Superoxide and Superoxide Dismutase in Chemistry, Biology and Medicine Elsevier Sci Publ Amsterdam 237–245

    Google Scholar 

  • TL Parkes AJ Elia D Dickinson AJ Hilliker JP Phillips GL Boulianne (1998) ArticleTitleExtension of Drosophila lifespan by overexpression of human SOD1 in motorneurons Nat. Genet. 19 171–174 Occurrence Handle10.1038/534 Occurrence Handle1:CAS:528:DyaK1cXls1Crurg%3D Occurrence Handle9620775

    Article  CAS  PubMed  Google Scholar 

  • U Rauen B Polzar H Stephan HG Mannherz H Groot Particlede (1999) ArticleTitleCold-induced apoptosis in cultured hepatocytes and liver endothelial cells: mediation by reactive oxygen species FASEB J. 13 155–168 Occurrence Handle1:CAS:528:DyaK1MXlt1ynug%3D%3D Occurrence Handle9872940

    CAS  PubMed  Google Scholar 

  • RS Sohol A Agarwal S Agarwal WC Orr (1995) ArticleTitleSimultaneous overexpression of copper- and zinc-containing superoxide dismutase and catalase retards age-related oxidative damage and increases metabolic potential in Drosophila melanogaster J. Biol. Chem. 270 15671–15674 Occurrence Handle10.1074/jbc.270.26.15671 Occurrence Handle7797567

    Article  PubMed  Google Scholar 

  • LA Sturtz K Diekert LT Jensen R Lill VC Culotta (2001) ArticleTitleA fraction of yeast Cu,Zn-superoxide dismutase and its metallochaperone, CCS, localize to the intermembrane space of mitochondria J. Biol. Chem. 276 38084–38089 Occurrence Handle1:CAS:528:DC%2BD3MXotFOrur0%3D Occurrence Handle11500508

    CAS  PubMed  Google Scholar 

  • J Sun J Tower (1999) ArticleTitleFLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies Mol. Cell. Biol. 19 216–228 Occurrence Handle1:CAS:528:DyaK1MXhvFOmsw%3D%3D

    CAS  Google Scholar 

  • DL Swofford (2000) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods), Version 4 Sinauer Sunderland MA

    Google Scholar 

  • JA Tainer ED Getzoff KM Beem JS Richardson DC Richardson (1982) ArticleTitleDetermination and analysis of the 2 Å structure of copper, zinc superoxide dismutase J. Mol. Biol. 160 181–217 Occurrence Handle10.1016/0022-2836(82)90174-7 Occurrence Handle1:CAS:528:DyaL38XlvFGisLk%3D

    Article  CAS  Google Scholar 

  • CW Whitfield MR Band MF Banaldo CG Kumar L Liu JR Pardinas HM Robertson MB Soares GE Robinson (2002) ArticleTitleAnnotated expressed sequence tags and cDNA microarrays for studies of brain and behavior in the honey bee Genome Res. 12 555–566 Occurrence Handle10.1101/gr.5302 Occurrence Handle11932240

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Biology, National Institute of Agricultural Science and Technology, Suwon, 441–100, Korea

    Iksoo Kim & Jae Sam Hwang

  2. College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea

    Kwang Sik Lee, Young Soo Choi, Hung Dae Sohn & Byung Rae Jin

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  1. Iksoo Kim
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  2. Kwang Sik Lee
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  3. Young Soo Choi
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  4. Jae Sam Hwang
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  5. Hung Dae Sohn
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  6. Byung Rae Jin
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Correspondence to Byung Rae Jin.

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Kim, I., Lee, K.S., Choi, Y.S. et al. Cloning and characterization of the Cu,Zn superoxide dismutase (SOD1) cDNA from the mole cricket, Gryllotalpa orientalis. Biotechnol Lett 27, 589–595 (2005). https://doi.org/10.1007/s10529-005-2885-4

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  • DOI: https://doi.org/10.1007/s10529-005-2885-4

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