Abstract
By using our previously optimized media and a fed-batch operation controlled by LabVIEW Software, the key parameter for a high production of alkaline protease using the marine bacterium, Teredinobacter turnirae, was to maintain a low concentration of C and N-sources ( < 2 g sucrose l−1 and < 0.2 g NH4C l l−1) using an appropriate fed-batch culture system. A maximum protease activity of 8250 U ml−1 was thus achieved.
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QK Beg RK Saxena R Gupta (2002) ArticleTitleDe-repression and consequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations Proc. Biochem. 37 1103–1109
U Beshay A Moreira (2003) ArticleTitleEffect of medium composition on the production of alkaline protease by Teredinobacter turnirae Deutsche Lebensmittel-Rundschau 8 319–323
GM Ferreira SK Ahuja AR Moreira (2001) ArticleTitlePleomorphism of the marine bacterium Teredinobacter turnirae Lett. Appl. Microbiol. 33 56–60
UE Giesecke G Bierbaum H Rudde U Spohn C Wandrey (1991) ArticleTitleProduction of alkaline protease with Bacillus licheniformis in a controlled fed-batch process Appl. Microbiol. Biotechnol. 35 720–724
HL Griffin RV Greene MA Cotta (1992) ArticleTitleIsolation and characterization of an alkaline protease from the marine shipworm bacterium Curr. Microbiol. 24 111–117
MM Kole I Draper DF Gerson (1988a) ArticleTitleProtease production by Bacillus subtilis in oxygen-controlled, glucose fed-batch fermentations Appl. Microbiol. Biotechnol. 28 404–408
MM Kole I Draper DF Gerson (1988b) ArticleTitleProduction of protease by Bacillus subtilis using simultaneous control of glucose and ammonium concentrations J. Chem. Tech. Biotechnol. 41 197–206
Z Liangqi Q Jing G Jinyang (1999) ArticleTitleA kinetics study on the production of alkaline protease by Bacillus licheniformis 2709 Chin. J. Biotechnol. 14 241–247
JB Waterbury CB Calloway RD Tutner (1983) ArticleTitleA cellulolytic nitrogen-fixing bacterium cultured from the gland of Deshayes in shipworms (Bivalvia: Teredinidae) Science 221 1401–1403
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Beshay, U., Moreira, A. Production of Alkaline Protease with Teredinobacter turnirae in Controlled Fed-batch Fermentation. Biotechnol Lett 27, 1457–1460 (2005). https://doi.org/10.1007/s10529-005-1309-9
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DOI: https://doi.org/10.1007/s10529-005-1309-9