Skip to main content
Log in

Thermostable cellulases from Streptomycessp.: scale-up production in a 50-l fermenter

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Thermostable cellulase was produced by Streptomyces sp. T3-1 grown in a 50-l fermenter. Maximum cellulase activity was attained on the fourth day when agitation speeds and aeration rates were controlled at 300 rpm and 0.75 vvm, respectively. Maximum enzyme activities were: 148 IU CMCase ml−1, 45 IU Avicelase ml−1, and 137 IU β-glucosidase ml−1 with productivity of 326 IU l−1 h−1, which were 10--32% higher than the values obtained in shake-flask cultures

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • MK Bhat S Bhat (1997) ArticleTitleCellulose degrading enzymes and their potential industrial applications Biotechnol. Adv. 15 583–620

    Google Scholar 

  • JM Fernandez-Abalos P Sanchez PM Coll JR Villanueva P Perez RI Santamaria (1992) ArticleTitleCloning and nucleotide sequence of celA1 and endo-β-1, 4-glucanase-encoding gene from Streptomyces halstedii JM8 J. Bacteriol. 174 6368–6376

    Google Scholar 

  • TK Ghose (1987) ArticleTitleMeasurement of cellulase activities Pure Appl. Chem. 59 257–268

    Google Scholar 

  • GD Haki GD Rakshit (2003) ArticleTitleDevelopments in industrially important thermostable enzymes: a review Bioresour. Technol. 89 17–34

    Google Scholar 

  • DA Hopwood MJ Bibb KF Chater CJ Bruton HM Kieser DJ Lydiate CP Smith JM Ward (1985) Genetic Manipulation of Streptomyces: A Laboratory Manual The John Innes Foundation Norwich, UK

    Google Scholar 

  • HD Jang KS Chen (2003) ArticleTitleProduction and characterization of thermostable cellulases from Streptomyces transformant T3-1 World J. Microbiol. Biotechnol. 19 263–268

    Google Scholar 

  • M Mandels ET Reese (1964) ArticleTitleFungal cellulase and the microbial decomposition of cellulosic fabric Dev. Ind. Microbiol. 5 5–20

    Google Scholar 

  • JA Milner DJ Martin A Smith (1996) ArticleTitleOxygen transfer conditions in the production of alpha-amylase by Bacillus amyloliquefaciens Enzyme Microb. Technol. 18 507–52

    Google Scholar 

  • F Niehaus C Bertoldo M Kahler G Antranikian (1999) ArticleTitleExtremophiles as a source of novel enzymes for industrial applications Appl. Microbiol. Biotechnol. 51 711–729

    Google Scholar 

  • H Schrempf S Walter (1995) ArticleTitleThe cellulolytic system of Streptomyces reticuli Int. J. Biol. Macromol. 17 353–355

    Google Scholar 

  • M Tuncer AS Ball A Rob MT Wilson (1999) ArticleTitleOptimization of extracellular lignocellulolytic enzyme production by a thermophilic actinomycetes Thermomonospora fusca BD25 Enzyme Microb. Technol. 25 38–47

    Google Scholar 

  • S Wittmann F Shareck D Kluepfel R Morosol (1994) ArticleTitlePurification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the encoding gene Appl. Environ. Microbiol. 60 1701–1703

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hung-Der Jang.

Additional information

Revisions requested 12 October 2004/1 November 2004; Received received 1 November 2004/14 December 2004

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jang, HD., Chang, KS. Thermostable cellulases from Streptomycessp.: scale-up production in a 50-l fermenter. Biotechnol Lett 27, 239–242 (2005). https://doi.org/10.1007/s10529-004-8356-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-004-8356-5

Keywords

Navigation