Abstract
As a malignant tumor, gastric cancer (GC) is closely related with gastric mucosa and has a high mortality in the world. Since microRNA (miRNA) has become more and more important in tumor research, we intend to find out the functional role and mechanism of miR-511-5p in GC. Firstly, miR-511-5p level was examined in human GC cell lines and tissues, and its effect on cell migration and invasion of BGC-823 or HGC-27 cells was tested by migration assay and transwell assay. Then, we confirmed the association between miR-511-5p and p21 activated kinase 2 (PAK2) by the luciferase reporter assay, and further assessed their role in cell migration and invasion. Moreover, we verified the function of miR-511-5p and PAK2 in epithelial–mesenchymal transition (EMT). In our study, miR-511-5p was downregulated in GC cell lines and tissues, and inversely associated with PAK2. Luciferase reporter assay confirmed that miR-511-5p could bind to PAK2. MiR-511-5p mimics significantly upregulated E-cadherin and downregulated N-cadherin, Vimentin and Snail, and consequently inhibited cell migration and invasion. However, reintroduction of PAK2 reversed the inhibitory function of miR-511-5p on BGC-823 and HGC-27 cells. Our research suggested that tumor-suppressive function of miR-511-5p in GC was inhibited by PAK2, and miR-511-5p/PAK2 axis may serve as a new strategy in GC management.
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Abbreviations
- EMT:
-
Epithelial–mesenchymal transition
- GADPH:
-
Glyceraldehyde-3-phosphate dehydrogenase
- GC:
-
Gastric cancer
- GEC-1:
-
Normal gastric epithelial cell lines
- miRNA:
-
microRNA
- MMPs:
-
Matrix metalloproteinases
- NC:
-
Negative control
- PAGE:
-
Polyacrylamide gels electrophoresis
- PAK2:
-
P21 activated kinase 2
- PVDF:
-
Polyvinylidene fluoride
- qRT-PCR:
-
Quantitative real-time polymerase chain reaction
- SDS:
-
Sodium dodecylsulfate
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10528_2021_10126_MOESM1_ESM.tif
Supplementary file1 (TIF 313 kb) Figure S1. Western blot analysis was used to detected PAK2 protein level in GC tissues. Results were shown as the mean ± SD of three separated exams. **p < 0.01.
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Yong, W., Zhang, K., Deng, Y. et al. miR-511-5p Suppresses Cell Migration, Invasion and Epithelial–Mesenchymal Transition Through Targeting PAK2 in Gastric Cancer. Biochem Genet 60, 899–913 (2022). https://doi.org/10.1007/s10528-021-10126-y
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DOI: https://doi.org/10.1007/s10528-021-10126-y