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Association of rs146292819 Polymorphism in ABCA1 Gene with the Risk of Coronary Artery Disease in Pakistani Population

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Abstract

Coronary artery disease (CAD) is an inflammatory heart disease characterized by the narrowing of coronary arteries. ATP-binding cassette transporter A1 (ABCA1) is a gene involved in regulation of cholesterol efflux and formation of high-density lipoprotein cholesterol (HDL-C). Present study aimed to explore the association of ABCA1 rs146292819 polymorphism with CAD development as well as its effect on serum lipid levels in the Pakistani population. Study subjects included 300 CAD patients and 300 age- and sex-matched healthy individuals. Methods involved genomic DNA extraction, amplification of rs146292819 polymorphism using allele-specific PCR, analyzing PCR product by agarose gel electrophoresis and determination of serum lipids. In this study, genotype frequencies of rs146292819 polymorphism in CAD patients were GG (43%), GT (27%), TT (30%) as compared to GG (25%), GT (31%), TT (44%) in healthy subjects. GG genotype increased the risk of developing CAD by 2.2326 times (OR 2.2326; 95% CI 1.5775–3.1597) and caused decrease in HDL-C levels by 2.6348 times. GT genotype was neither associated with CAD development (OR 0.8504; 95% CI 0.5974–1.2106) nor HDL-C levels. TT genotype lowered the risk of CAD development by 0.5381 times (OR 0.5381; 95% CI 0.3846–0.753) and protected from drop in HDL-C levels by 0.5086 times (OR 0.5086; 95% CI 0.3429–0.7544). It can be concluded that GG genotype of rs146292819 polymorphism and altered lipid profile act as risk factors in the pathogenesis of CAD in the Pakistani population.

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Acknowledgements

We are thankful to Department of Zoology, University of Sargodha, Sargodha, Pakistan, for providing well-equipped lab and facilitating us during research work.

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Authors and Affiliations

  1. Department of Zoology, University of Sargodha, Sargodha, Pakistan

    Maryam Sameem & Asima Rani

  2. Department of Zoology, University of Education, Lower Mall Campus, Lahore, Pakistan

    Muhammad Arshad

Authors
  1. Maryam Sameem
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  2. Asima Rani
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  3. Muhammad Arshad
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Contributions

M.S. designed the study, performed lab work, statistically analyzed the data, and wrote the manuscript. A.R. helped in data collection and primer designing for PCR. M.A. edited the manuscript and approved the final manuscript for publication.

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Correspondence to Maryam Sameem.

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Sameem, M., Rani, A. & Arshad, M. Association of rs146292819 Polymorphism in ABCA1 Gene with the Risk of Coronary Artery Disease in Pakistani Population. Biochem Genet 57, 623–637 (2019). https://doi.org/10.1007/s10528-019-09915-3

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  • DOI: https://doi.org/10.1007/s10528-019-09915-3

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