Abstract
The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydın, Muğla, İzmir, Denizli, Kahramanmaraş, Osmaniye, Eskişehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs. Among the 470 bands generated by the RAPD primers, 467 were polymorphic. The number of bands detected by a single primer set ranged from 11 to 22 (average of 15.6). The percentage polymorphism was 99.3 % based on the RAPD data. In the SRAP analysis, a total of 216 bands were generated, showing 100 % polymorphism. The number of bands detected by a single primer set ranged from 9 to 22 (average of 15.4). All data were scored and UPGMA dendrograms were constructed with similar results in both marker systems, i.e., different species from nine provinces of Turkey were separated from each other in the dendrograms with the same species being clustered together.
Similar content being viewed by others
References
Aalaey M, Naderi R, Khalighi A (2007) Morphological categorization of some cyclamens from Iran through studying and comparing the quantitative and qualitative traits. Iran J Agr Sci 37:385–393
Aka Kacar Y, Darıcı, HC, Söğüt Z, Solmaz İ, Bozdoğan E, Haspolat G, Teixeira da Silva JA (2013) Molecular characterization and in vitro preservation of Cyclamen Species Grown Naturally in Turkey Project No: 1100102 Final Report (In Turkish), pp 1–157
Aka Kacar Y, Demirel A, Tuzcu O, Yesiloglu T (2005) Preliminary results on fingerprinting lemon genotypes tolerant to mal secco (Phoma tracheiphila Kanc. et Ghik) disease by RAPD markers. Biologia (sect. Mol. Biol.) 60:295–300
Amar MH, Biswas MK, Zhang Z, Guo WW (2011) Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of Citrus germplasm collection. Sci Hortic 128(3):220–227
Anderberg AA (1994) Phylogeny and subgeneric classification of Cyclamen L. (Primulaceae. Kew Bul 49(3):455–467
Compton JA, Clennett JCB, Culham A (2004) Nomenclature in the dock. Overclassification leads to instability: a case study in the horticulturally important genus Cyclamen (Myrsinaceae). Bot J Linn Soc 146:339–349
Curuk P, Sogut Z, Bozdogan E, Izgu T, Sevindik B, Tagipur E, Teixeira da Silva JA, Serce S, Aka Kacar Y, Mendi Y (2015) Morphological characterization of Cyclamen sp. grown naturally in Turkey: part I. S Afr J Bot 100:7–15
Debussche M, Thompson JD (2002) Morphological differentiation among closely related species with disjunct distributions: a case study of Mediterranean Cyclamen L. subgen. Psilanthum Schwarz (Primulaceae). Bot J Linn Soc 139:133–144
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349
Gielly L, Debussche M, Thompson JD (2001) Geographic isolation and evolution of Mediterranean endemic Cyclamen: insights from chloroplast trnL (UAA) intron sequence variation. Plant Syst Evol 230:75–88
Grey-Wilson C (1997) Cyclamen. A guide for gardeners, horticulturists and botanists. B.T. Batsford Ltd., London, pp 192
Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1):9
Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270
Jalali N, Naderi R, Ali SG, Teixeira da Silva JA (2012) Cyclamen tissue culture. Sci Hortic 137:11–19
Kallersjo M, Bergqvist G, Anderberg AA (2000) Generic realignment in primuloid families of the Ericales s.l.: a phylogenetic analysis based on DNA sequences from three chloroplast genes and morphology. Am J Bot 87:1325–1341
Kitamura S, Akita Y, Ishizaka H, Narumi I, Tanaka A (2012) Molecular characterization of an anthocyanin-related glutathione S-transferase gene in cyclamen. J Plant Physiol 169:636–642
Laura M, De Benedetti L, Bruna S, Burchi G, Berio T, Giovannini A, Ruffoni B (2003) Cyclamen persicum Mill. Somatic embryogenesis and RAPD analysis of embryogenic callus. Acta Hort 625:137–144
Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP) a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461
Naderi R, Alaey M, Khalighi A, Hassani ME, Salami SA (2009) Inter-and intra-specific genetic diversity among cyclamen accessions investigated by RAPD markers. Sci Hortic 122:658–661
Rohlf FJ (1998) NTSYS-PC Numerical taxonomy and multivariate analysis system. Version 2.00 Exeter software Setauket, New York
Smith JSC, Chin ECL, Shu H, Smith OS, Wall SJ, Senior ML, Mitchel SE, Kresorich S, Tiegle J (1997) An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theor Appl Genet 95:163–173
Taskin BG, Vardareli N, Doğaç E, Mammadov R, Taskin V (2012) Genetic diversity of natural Cyclamen alpinum populations. Turk J Biol 36(4):413–422
Uzun A, Yesiloglu T, Aka-Kacar Y, Tuzcu O (2009) Genetic diversity and relationships within Citrus and related genera based on sequence related amplified polymorphism markers (SRAPs). Sci Hortic 121:306–312
Zhang JH, McDonald MB, Sweeney PM (1997) Testing for genetic purity in petunia and cyclamen seed using random amplified polymorphic DNA markers. Hort Science 32:246–247
Acknowledgments
This research was funded by the Scientific and Technical Research Council of Turkey (TÜBİTAK, project number 110O102).
Author information
Authors and Affiliations
Corresponding author
Additional information
An erratum to this article is available at http://dx.doi.org/10.1007/s10528-016-9774-5.
Rights and permissions
About this article
Cite this article
Simsek, O., Curuk, P., Aslan, F. et al. Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers. Biochem Genet 55, 87–102 (2017). https://doi.org/10.1007/s10528-016-9770-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10528-016-9770-9