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Genetic Diversity Among Historical Olive (Olea europaea L.) Genotypes from Southern Anatolia Based on SSR Markers

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Abstract

Olive (Olea europaea) is an ancient and important crop in both olive oil production and table use. It is important to identify the genetic diversity of olive genetic resources for cultivar development and evaluation of olive germplasm. In the study, 14 microsatellite markers (UDO4, UDO8, UDO9, UDO11, UDO12, UDO22, UDO24, UDO26, UDO28, DCA9, DCA11, DCA13, DCA15, and DCA18) were used to assess the genetic variation on 76 olive (Olea europaea L.) genotypes from Mardin province together with 6 well-known Turkish and 4 well-known foreign reference cultivars. All microsatellite markers showed polymorphism and the number of alleles varied between 9 and 22, with an average of 14.57. The most informative loci were DCA 11 (22 alleles) and DCA 9 (21 alleles). Dendrogram based on genetic distances was constructed for the 86 olive genotypes/cultivars, which revealed the existence of different clusters. The high genetic similarity was evident between Bakırkire2 and Zinnar5 (0.74) genotypes, while the most genetically divergent genotypes were Gürmeşe5 and Yedikardeşler2 (0.19). It was concluded that there was abundant SSR polymorphism in olive germplasm in southern Anatolia in Turkey and could be important for future breeding activities.

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References

  • Abdessemed S, Muzzalupo I, Benbouza H (2015) Assessment of genetic diversity among Algerian olive (Olea europaea L.) cultivars using SSR marker. Sci Hortic 192:10–20

    Article  Google Scholar 

  • Alba V, Montemurro C, Sabetta W, Pasqualone A, Blanco A (2009) SSR-based identification key of cultivars of Olea europaea L. diffused in Southern-Italy. Sci Hortic 123:11–16

    Article  Google Scholar 

  • Albertini R, Torricelli E, Bitocchi E, Raggi L, Marconi G, Pollastri L, Di Minco G, Battistini A, Papa R, Veronesi F (2011) Structure of genetic diversity in Olea europaea L. cultivars from central Italy. Mol Breed 27(4):533–547

    Article  Google Scholar 

  • Barranco D, Cimato A, Fiorino P, Rallo L, Touzani A, Castaneda C, Serafin F, Trujillo I (2000) World catalogue of olive varieties. International Olive Oil Council, Madrid 360 pp

    Google Scholar 

  • Belaj A, Satovic Z, Cipriani G, Baldoni L, Testolin R, Rallo L, Trujillo I (2003) Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and their effectiveness in establishing genetic relationships in olive. Theor Appl Genet 107:736–744

    Article  CAS  PubMed  Google Scholar 

  • Besnard G, Baradat P, Berville A (2001) Genetic relationships in the olive (Olea europaea L.) reflect multilocal selection of cultivars. Theor Appl Genet 102:251–258

    Article  CAS  Google Scholar 

  • Bowcock AM, Ruiz-Linares A, Tomfohrde T, Minch E, Kidd JR, Cavalli-Sforza LL (1994) High resolution of human evolutionary trees with polymorphic microsatellites. Nature 368:455–457

    Article  CAS  PubMed  Google Scholar 

  • Cheng XM, Xu JS, Xia S, Gu JX, Yang Y, Fu J, Qian XJ, Zhang SC, Wu JS, Liu KD (2009) Development and genetic mapping of microsatellite markers froö genome survey sequences in Brassica napus. Theor Appl Genet 118:1121–1131

    Article  CAS  PubMed  Google Scholar 

  • Cipriani G, Marrazzo MT, Marconi R, Cimato A, Testolin R (2002) Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theor Appl Genet 104:223–228

    Article  CAS  PubMed  Google Scholar 

  • Contento A, Ceccarelli M, Gelati MT, Maggini F, Baldoni L, Cionini PG (2002) Diversity of olea genotypes and the origin of cultivated olives. Theor Appl Genet 104:1229–1238

    Article  CAS  PubMed  Google Scholar 

  • Ercisli S, Ipek A, Barut E (2011) SSR marker-based DNA fingerprinting and cultivar identification of olives (Olea europaea L.). Biochem Genet 49(9–10):555–561

    Article  CAS  PubMed  Google Scholar 

  • Ipek A, Barut E, Gulen H, Ipek M (2012) Assessment of inter and intra-cultivar variations in olive using SSR markers. Sci Agricola 69:327–335

    Article  CAS  Google Scholar 

  • Kaczmarska E, Gawronski J, Dyduch-Sieminska M, Najda A, Marecki W, Zebrowska J (2015) Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea). Turk J Agric For 39:394402

    Article  Google Scholar 

  • Kaya HB, Cetin O, Kaya H, Sahin M, Sefer F, Kahraman A, Tanyolac B (2013) SNP discovery by Illumina-based transcriptome sequencing of the olive and the genetic characterization of Turkish olive genotypes revealed by AFLP, SSR and SNP markers. PloS One 8:e73674

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lopes MS, Mendonca D, Sefc KM, Sabino Gil F, da Camara Machado A (2004) Genetic evidence of intra-cultivar variability within Iberian olive cultivars. HortScience 39:1562–1565

    CAS  Google Scholar 

  • Minch E, Ruiz-Linares A, Goldstein DB, Feldman M, Cavalli-Sforza LL (1995) Microsat (Version 1.4d): a computer program for calculating various statistics on microsatellite allele data. Stanford University Medical Center, Stanford

    Google Scholar 

  • Mishra PK, Ram RB, Kumar N (2015) Genetic variability, heritability, and genetic advance in strawberry (Fragaria × ananassa Duch.). Turk J Agric For 39:451–458

    Article  Google Scholar 

  • Mlcek J, Valsikova M, Druzbikova H, Ryant P, Jurikova T, Sochor J, Borkovcova M (2015) The antioxidant capacity and macroelement content of several onion cultivars. Turk J Agric For 39:999–1004

    Article  Google Scholar 

  • Muzzalupo I, Vendramin GG, Chiappetta A (2014) Genetic biodiversity of Italian olives (Olea europaea) germplasm analyzed by SSR markers. Sci World J 116(3):352–359

    Google Scholar 

  • Nemli S, Kianoosh T, Tanyolaç MD (2015) Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers. Turk J Agric For 39:940–948

    Article  Google Scholar 

  • Noormohammadi Z, Hosseini-Mazinani M, Trujillo I, Belaj A (2009) Study of intracultivar variation among main Iranian olive cultivars using SSR markers. Acta Biol Szeged 53(1):27–32

    Google Scholar 

  • Paetkau D, Calvert W, Stirling I, Strobeck C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354

    Article  CAS  PubMed  Google Scholar 

  • Poljuha D, Sladonja B, Setic E, Milotic A, Bandelj D, Jakse J, Javornik B (2008) DNA fingerprinting of olive varieties in Istria (Croatia) by microsatellite markers. Sci Hortic 115:223–230

    Article  CAS  Google Scholar 

  • Rohlf FJ (2004) NTSYS-pc numerical taxonomy and multivariate analysis system. Version 2.11V. Exeter software, Setauket

    Google Scholar 

  • Roubos K, Moustakas M, Aravanopoulos FA (2010) Molecular identification of greek olive (Olea europaea) cultivars based on microsatellite loci. Genet Mol Res 9(3):1865–1876

    Article  CAS  PubMed  Google Scholar 

  • Sakar E, Celik M, Ergul A (2013) Molecular analysis of olive germplasms grown in Adiyaman based on SSR markers. Zeytin Dostu 27:78–83

    Google Scholar 

  • Sneath PHA, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. Freeman, San Francisco

    Google Scholar 

  • Zaher H, Boulouha B, Baaziz M, Sikaoui L, Gaboun F, Udupa SM (2011) Morphological and genetic diversity in olive (Olea europaea subsp. europaea L.) clones and varieties. Plant Omics J 4(7):370–376

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Horticulture, Agricultural Faculty, Harran University, Sanliurfa, Turkey

    Ebru Sakar

  2. Faculty of Agriculture and Natural Science, Duzce University, Duzce, Turkey

    Hulya Unver

  3. Department of Horticulture, Agricultural Faculty, Ataturk University, Erzurum, Turkey

    Sezai Ercisli

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  1. Ebru Sakar
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  2. Hulya Unver
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  3. Sezai Ercisli
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Correspondence to Hulya Unver.

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Sakar, E., Unver, H. & Ercisli, S. Genetic Diversity Among Historical Olive (Olea europaea L.) Genotypes from Southern Anatolia Based on SSR Markers. Biochem Genet 54, 842–853 (2016). https://doi.org/10.1007/s10528-016-9761-x

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  • DOI: https://doi.org/10.1007/s10528-016-9761-x

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