Abstract
Heat shock proteins (Hsps) are a family of highly conserved proteins present in all organisms. They mediate a range of cytoprotective functions as molecular chaperones and are recently reported to regulate the immune response. Using suppression subtractive hybridization, we isolated and characterized a cucumber cDNA, designated CsHsp45.9, which encodes a putative heat shock protein of 45.9 kDa protein, containing three conserved DnaJ domains belonging to the Type I Hsp40 family. Real-time quantitative RT-PCR analysis revealed that CsHsp45.9 was significantly induced in cucumber leaves inoculated with downy mildew (Pseudoperonospora cubensis) in this incompatible interaction. Gene expression was also strongly up-regulated by various abiotic stresses. CsHsp45.9 was mainly expressed in flowers with a flower-specific, stamen- and pistil-predominant expression pattern. This suggests that CsHsp45.9 harbors broad-spectrum responses to both biotic and abiotic stresses and may play a role in downy mildew resistance in cucumber.
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Acknowledgments
This work was supported by Key Projects in the National Science & Technology Pillar Program during Eleventh Five-Year Plan (2009BADB8B02), Key Science & Research Program of Henan Province (No. 102102110037), and the National High Technology Project of China (2007AA10Z177). We are grateful to Dr. Zhiqi Jia for her technical assistance.
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Li, J., Zhang, H., Hu, J. et al. A Heat Shock Protein Gene, CsHsp45.9, Involved in the Response to Diverse Stresses in Cucumber. Biochem Genet 50, 565–578 (2012). https://doi.org/10.1007/s10528-012-9501-9
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DOI: https://doi.org/10.1007/s10528-012-9501-9