Abstract
Latent transforming growth factor-β binding protein-1 (LTBP1) has been implicated in the control of secretion, localization, and activation of TGFβ (transforming growth factor-β). We developed a quantitative reverse-transcriptase polymerase chain reaction (Q-RT-PCR) assay using an RNA internal standard to examine the expression of three alternatively spliced isoforms of LTBP1 (LTBP1Δ41, LTBP1Δ53, and LTBP1Δ55) in a variety of human tissues. The assays were also used to determine the expression of LTBP1L and LTBP1S isoforms and total LTBP1. The Q-RT-PCR assays were highly reproducible and showed that in most tissues LTBP1Δ55 and LTBP1L were minor components of LTBP1. The proportion of LTBP1Δ41 ranged from 2% of total LTBP1 mRNA in early coronary atherosclerotic lesions to 54% in advanced lesions.
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Acknowledgments
This work was supported by a British Heart Foundation program grant to JCM. RÖ is the recipient of a Koç scholarship from Ali Y. Koç, Koç Holding, Istanbul, Turkey. We are grateful to Drs. Chris Byrne and Junlong Zhang for helpful discussion of their Q-RT-PCR method.
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Öklü, R., Hesketh, R., Wicky, S. et al. Expression of mRNA Isoforms of Latent Transforming Growth Factor-β Binding Protein-1 in Coronary Atherosclerosis and Human Tissues. Biochem Genet 49, 213–225 (2011). https://doi.org/10.1007/s10528-010-9400-x
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DOI: https://doi.org/10.1007/s10528-010-9400-x