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Decreased total carbonic anhydrase esterase activity and decreased levels of carbonic anhydrase 1 isozyme in erythrocytes of type II diabetic patients

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In this exploratory study, we investigated total erythrocyte carbonic anhydrase (CA) estrase activity as well as CA I isozyme concentration in patients with diabetes mellitus type II (DM) and healthy individuals of Howard University Hospital community. Total estrase activity of CA was measured spectrophotometrically using p-nitrophenol acetate before and after inhibition with acetazolamide. CA I isozyme was measured by radial immunodiffusion using monoclonal antibody (CA I) in agarose plates. The study involved 20 consented participants; 10 normal (N) and 10 (DM), 21 to 84 years of age. The study was approved by the Howard University Institution Review Board. The CA activity was measured following lysis of cells as U/min/mL and CA I concentration as mg/l. We observed CA activity as 46.3±4(N) and 25±2.1 (DM) whereas CA I concentration as 1896±125 (N) and 1104 ±63 (DM). We speculate that the change in the CA activity may of fundamental importance in the regulation of intracellular; pHi for the basic control of metabolism in diabetes mellitus. Further, we propose that CA activity is a good candidate for a biomarker of diabetes mellitus for the early detection of insulin resistance because the CA activity variation was proportional to the severity of the diabetes.

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Acknowledgments

The authors sincerely appreciate the editing and typing of the manuscript by Ms. Adriana Malheiro. The authors thank Mr. Stafford L. Battle, Digital Designer in the office of curriculum operations, for his help in formatting the figures in a publishable form. The authors also wish to acknowledge the partial funding of laboratory supplies by grant 2G12RR003048 from the RCMI Program, Division of Research Infrastructure, and the National Center for Research Resources, NIH. A part of this study was published as an abstract in Clinical Chemistry (Gambhir et al. 2003).

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Authors and Affiliations

  1. Molecular Endocrinology Laboratory, Department of Medicine, College of Medicine, and Department of Genetics and Human Genetics, Graduate School, Howard University, Washington, DC, 20060, USA

    Kanwal K. Gambhir

  2. Department of Genetics and Human Genetics, Graduate School, Howard University, Washington, DC, 20059, USA

    Jehan Ornasir

  3. Department of Genetics and Human Genetics, College of Medicine and Graduate School, Howard University, Washington, DC, 20059, USA

    Verle Headings

  4. Divison of Endocrinology and Metabolism, Department of Medicine, College of Medicine, and Department of Genetics and Human Genetics, Graduate School, Howard University, Washington, DC, 20060, USA

    Adolphus Bonar

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  1. Kanwal K. Gambhir
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  2. Jehan Ornasir
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  3. Verle Headings
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  4. Adolphus Bonar
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Corresponding author

Correspondence to Kanwal K. Gambhir.

Additional information

Jehan Ornasir—these studies were undertaken as a partial requirement of her M.S. Degree, Graduate School, Howard University, Washington, DC, USA

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Gambhir, K.K., Ornasir, J., Headings, V. et al. Decreased total carbonic anhydrase esterase activity and decreased levels of carbonic anhydrase 1 isozyme in erythrocytes of type II diabetic patients. Biochem Genet 45, 431–439 (2007). https://doi.org/10.1007/s10528-007-9086-x

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  • DOI: https://doi.org/10.1007/s10528-007-9086-x

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