Abstract
A novel human zinc finger protein encoding gene ZNF468 was obtained from a fetal brain cDNA library. By BLAST-N analysis we found two different splice variants. We termed the two splice variants ZNF468.1 and ZNF468.2. By BLAST search against the human genome database, ZNF468 was mapped to 19q13.4. The ZNF468.1 cDNA has four exons, and the ZNF468.2 cDNA has one more, between the third and fourth exon. This extra exon creates a difference between the deduced protein N-termini of the two splice variants. The ZNF468.1 cDNA is 3906 bp in length, encoding a 522a a protein, and ZNF468.2 is 4024 bp, encoding a 469-aa-protein. Both proteins contain 11 C2H2-type zinc finger motifs at their C-termini. The N-terminus of the deduced protein of ZNF468.1 has a well-conserved Krüppel-associated box (KRAB) domain that consists of KRAB boxes A and B, whereas the protein of ZNF468.2 does not have the {KRAB} domain. Tissue distribution of the ZNF468 gene indicates that the two splice variants are widely expressed in normal human tissues, except in heart and brain, and they are also co-expressional.
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Abrink, M., Ortiz, José A., Mark, C., Sanchez, C., Looman, C., Hellman, L., Chambon, P., and Losson, R. (2001). Conserved interaction between distinct Krüppel-associated box domains and the transcriptional intermediary factor 1β. PNAS 98:1422–1426.
Friedman, J. R., Fredericks, W. J., Jensen, D. E., Speicher, D. W., Huang, X. P., Neilson, E. G., and Rauscher, F. J. 3rd (1996). KAP-1, a novel corepressor for the highly conserved KRAB repression domain. Genes Dev. 10:2067–78.
Imanishi, M. K., Hori, Y., Nagaoka, M., and Sugiura, Y. (2001). Design of novel zinc finger proteins: Towards artificial control of specific gene expression. Eur. J. Pharm. Sci. 13:91–97.
Kim, S.-S., Chen, Y.-M., O'Leary, E., Witzgall, R., Vidal, M., and Bonventre, J. V. (1996). A novel member of the RING finger family, KRIP-1, associates with the KRAB-A transcriptional repressor domain of zinc finger proteins. Proc. Natl. Acad. Sci. USA 93:15299–15304.
Kozak, M. (1996). Interpreting cDNA sequences: Some insights from studies on translation. Mamm. Genome 7:563–574.
Mark, C., Abrink, M., and Hellman, L. (1999). Comparative analysis of KRABA zinc finger proteins in rodents and man: evidence for several evolutionarily distinct subfamilies of KRAB zinc finger genes. DNA Cell Biol. 18:381–396.
Tian, Y., Breedveld, G. J., Huang, S. Z., Oostra, B. A., Heutink, P., and Lo, W. H. Y. (2002). Characterization of ZNF333, a novel double KRAB domain containing zinc finger gene on human chromosome 19p13.1. Biochim. Biophys. Acta 1577:121–125.
Urrutia, R. (2003). KRAB-containing zinc-finger repressor proteins. Genome Biol 4:231.
Vissing, H., Meyer, W. K.-H., Aagaard, L., Tommerup, N., and Thiesen, H.-J. (1995) Repression of transcriptional activity by heterologous KRAB domain present in zinc finger proteins. FEBS Lett. 369:153–157.
Wu, H., Zhang, S. Z., Qiu, W. M., Zhang, G., and Xia, Q. J., (2001) Isolation, characterization, and mapping of a novel human KRAB zinc finger protein encoding gene ZNF463. Biochim. Biophys. Acta 1518:190–193.
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Sun, L., Gu, S., Li, N. et al. A Novel Zinc Finger Gene ZNF468 with Two Co-Expressional Splice Variants, ZNF468.1 and ZNF468.2. Biochem Genet 43, 271–286 (2005). https://doi.org/10.1007/s10528-005-5219-2
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DOI: https://doi.org/10.1007/s10528-005-5219-2