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Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria

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An Erratum to this article was published on 23 July 2010

Abstract

Bacterial strains with ability to suppress Colletotrichum falcatum were isolated from the rhizosphere of sugarcane. Thirty nine candidates, chosen on the basis of in vitro antagonism, inhibited C. falcatum growth by 15–65% on test plates. Twenty two isolates causing 50% or more in vitro inhibition were screened for their root colonization ability and biocontrol activity on micropropagated sugarcane plants under greenhouse conditions. Twelve strains suppressed red rot infection in plantlets, but no significant correlation was observed between in vitro pathogen inhibition and in vivo disease suppression. However, isolates showing root colonization over 5.2 log10 CFU g−1 of soil showed highest suppression of C. falcatum and reduction of red rot disease. Six strains with the capability to maintain a significant population in the sugarcane rhizosphere and with a high potential to control red rot were identified by 16S rDNA as Ochrobacterum intermedium NH-5, Pseudomonas putida NH-50, Bacillus subtilis NH-100, Bacillus subtilis NH-160, Bacillus sp NH-217 and Stenotrophomonas maltophilia NH-300.

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Acknowledgments

This work was partially supported by the Higher Education Commission (HEC) Pakistan under project “Indigenous Ph.D 5000 Batch-II” and research grant#544. We are thankful to Dr. G. Defago (Plant pathology, Institute of Integrative Biology Zürich, Switzerland) for providing strain P. fluorescens CHA0 to be used as positive control in this study. We would like to say word of thanks to Dr. Michael Roberts (University of Lancaster, UK) for editing the English style of manuscript and Dr. Kauser Nawaz Shah (COMSATS Institute of Information Technology, Islamabad- Pakistan) for statistical analysis of data.

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  1. Muhammad Nadeem Hassan & Fauzia Yusuf Hafeez

    Present address: Department of Biosciences, COMSATS Institute of Information Technology, Chak Shahzad Campus, Park road, Islamabad, Pakistan

Authors and Affiliations

  1. National Institute For Biotechnology and Genetic Engineering (NIBGE), P. O. Box 577, Jhang Road, Faisalabad, Pakistan

    Muhammad Nadeem Hassan & Fauzia Yusuf Hafeez

  2. Shakarganj Sugar Research Institute (SSRI), Jhang, Pakistan

    Shahid Afghan

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  1. Muhammad Nadeem Hassan
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  2. Shahid Afghan
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Correspondence to Fauzia Yusuf Hafeez.

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Handling editor: Monica Hofte

An erratum to this article can be found at http://dx.doi.org/10.1007/s10526-010-9298-6

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Hassan, M.N., Afghan, S. & Hafeez, F.Y. Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria. BioControl 55, 531–542 (2010). https://doi.org/10.1007/s10526-010-9268-z

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  • DOI: https://doi.org/10.1007/s10526-010-9268-z

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