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Chitinase-mediated destructive antagonistic potential of Pseudomonas aeruginosa GRC1 against Sclerotinia sclerotiorum causing stem rot of peanut

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Abstract

Pseudomonas aeruginosa GRC1 exhibited strong antagonistic activity against Sclerotinia sclerotiorum, in vitro and in vivo. Scanning electron microscopic (SEM) studies showed morphological abnormalities such as perforation, lysis and fragmentation of hyphae of S. sclerotiorum caused by P. aeruginosa GRC1. This strain produced extracellular chitinase enzyme, the role of which was clearly demonstrated through Tn5 mutagenesis. Bacterization of peanut seeds with GRC1 resulted in increased seed germination and reduced stem-rot of peanut in S. sclerotiorum-infested soil by 97%. Other vegetative and yield plant parameters such as nodules per plant, pods and grain yield per plant were enhanced with a statistical significance in comparison to control. Neomycin resistant (GRC neo+1 ) bacterium was a good root colonizer and frequently isolated from rhizosphere of peanut plants. These findings showed P. aeruginosa GRC1 as a potential biocontrol agent against S. sclerotiorum.

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Abbreviations

SEM:

scanning electron microscopy

HCN:

hydrocyanic acid

CAS:

chrome azurol S

IAA:

indole acetic acid

TSM:

tryptic soy medium

PDA:

potato dextrose agar

CMM:

chitin minimal medium

Km:

kanamycin

neo:

neomycin

CMC:

carboxymethylcellulose

CFU:

colony forming unit

DAS:

days after sowing

ANOVA:

analysis of variants

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Acknowledgements

We thank Dr A. Ambani for the help with the Scanning Electron photo microscopy. This work was financially supported by TMOP - CSIR, New Delhi.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Gurukul Kangri University, Hardwar, 249404, India

    C. P. Gupta, Bhavesh Kumar, R. C. Dubey & D. K. Maheshwari

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  1. C. P. Gupta
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  2. Bhavesh Kumar
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  3. R. C. Dubey
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  4. D. K. Maheshwari
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Correspondence to D. K. Maheshwari.

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Gupta, C.P., Kumar, B., Dubey, R.C. et al. Chitinase-mediated destructive antagonistic potential of Pseudomonas aeruginosa GRC1 against Sclerotinia sclerotiorum causing stem rot of peanut. BioControl 51, 821–835 (2006). https://doi.org/10.1007/s10526-006-9000-1

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  • DOI: https://doi.org/10.1007/s10526-006-9000-1

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