Abstract
Mutual effects between the symbiotic bacteria of entomopathogenic nematodes, Photorhabdus luminescens and Xenorhabdus poinarii, and entomopathogenic fungi were investigated in vitro. A dual culture assay on nutrient agar supplemented with bromothymol blue and triphenyltetrazolium chloride (NBTA) medium revealed that P. luminescens is antagonistic to Metarhizium anisopliae, Beauveria bassiana, B. brongniartii and Paecilomyces fumosoroseus by inhibiting their growth and conidial production; the fungal growth was not inhibited by X. poinarii. In a second laboratory experiment, crude extract produced by M. anisopliae was tested for its activity against P. luminescens and X. poinarii. Crude extract from M. anisopliae was antibacterial to P. luminescens and X. poinarii at 1000 μg/ml and inhibited their growth on NBTA, but had no effect at 100 or 10 μg/ml. The influence of the crude extract of M. anisopliae on the dispersal of infective juveniles (IJs) of Heterorhabditis megidis and Steinernema glaseri was assayed on Sabouraud Dextrose Agar (SDA) plates. Results showed that the crude extract of M. anisopliae had no toxic effects even at highest concentration (1000 μg/ml).
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R.J. Akhurst G.B Dunphy (1993) Tripartite interactions between symbiotically associated entomopathogenic bacteria, and their insect hosts N.E. Beckage S.N. Thompson B.A. Federic (Eds) Parasites and Pathogens of Insects Academic Press, San Diego 1–23
R.J. Akhurst N.E. Boemare (1990) Biology and taxonomy of Xenorhabdus R. Gaugler H.K. Kaya (Eds) Entomopathogenic Nematodes in Biological Control. CRC Press Boca Raton, FL, USA 75–87
B. Amiri L. Ibrahim T.M. Butt (1999) ArticleTitleAntifeedant properties of destruxins and their use with the entomopathogenic fungi Metarhizium anisopliae for improved control of crucifer pests Biocontrol Sci. Technol. 9 487–498
M.A. Ansari L. Tirry M. Moens (2003) ArticleTitleEntomopathogenic nematodes and their symbiotic bacteria for the biological control of Hoplia philanthus (Coleoptera: Scarabaeidae) Biol. Control 28 111–117
M.A. Ansari L. Tirry S. Vestergaard M. Moens (2004) ArticleTitleSelection of a highly virulent fungal isolate, Metarhizium anisopliae CLO 53, for controlling Hoplia philanthus J. Invertebr. Pathol. 85 89–96 Occurrence Handle1:STN:280:DC%2BD2c7lsl2jtg%3D%3D Occurrence Handle15050838
M.A. Ansari L. Tirry M. Moens (2004) ArticleTitleInteraction between Metarhizium anisopliae CLO 53 and entomopathogenic nematodes for control of Hoplia philanthus Biol. Control 31 172–180
A.R. Bandani B.P.S. Khambay J. Faull R. Newton M. Deadman T.M. Butt (2000) ArticleTitleProduction of efrapeptins by Tolypocladium species and evaluation of their insecticidial and antimicrobial properties Mycol. Res. 104 537–544 Occurrence Handle1:CAS:528:DC%2BD3cXltFOlsr0%3D
M.E. Barbercheck H.K. Kaya (1990) ArticleTitleInteraction between Beauveria bassiana and the entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis heliothidis J. Invertebr. Pathol. 55 225–234
M.E. Baur H.K. Kaya B.E. Tabashnik C.R. Chilcutt (1998) ArticleTitleSuppression of diamondback moth (Lepidoptera: Plutellidae) with an entomopathogenic nematodes (Rhabditida: Steinernematidae) and Bacillus thuringiensis Berliner J. Econ. Entomol. 91 1089–1095 Occurrence Handle1:STN:280:DyaK1M%2Fit1ensg%3D%3D Occurrence Handle9805498
N.E. Boemare (2002) Biology, taxonomy and systematics of Photorhabdus and Xenorhabdus R. Gaugler (Eds) Entomopathogenic Nematology CABI Publishing Wallingford, UK 35–56
D.J. Bowen T.A. Rocheleau M. Blackburn O. Andreey E. Golubeva R. Bhartia R.H. ffrench-Constant (1998) ArticleTitleInsecticidal toxins from the bacterium Photorhabdus luminescens Science 280 2129–2132 Occurrence Handle1:CAS:528:DyaK1cXkt1Gks78%3D Occurrence Handle9641921
Butt, T.M., C. Jackson and N. Magan, 2001. Fungi as Biocontrol Agents: Progress, Problems and Potential, CABI International
G. Chen G.B. Dunphy J.M. Webster (1994) ArticleTitleAntifungal activity of two Xenorhabdus species and Photorhabdus luminescens species of Heterorhabditis megidis Biol. Control 4 157–162
C. Dumas V. Matha J.M. Quiot A. Vey (1996) ArticleTitleEffect of destruxins, cyclic depsipeptide mycotoxins, on calcium balance and phosphorylation of intracellular proteins in lepidopteron cell lines Compar Biochem. Physiol. 114 213–219 Occurrence Handle1:STN:280:DyaK2s%2FgvFymtA%3D%3D
S. Fort D. Clarke (2002) Bacteria–nematode symbiosis R. Gaugler (Eds) Entomopathogenic Nematology. CABI Publishing Wallingford, UK, 57–77
S. Goettel G.D. Inglis (1997) Fungi: Hyphomycetes. L.A. Lacey (Eds) Manual of Techniques in Insect Pathology. Academic Press London 213–249
M.S. Goettel G.D. Inglis S.P. Wraight (2000) Fungi L.A. Lacey H.K. Kaya (Eds) Field Manual of Techniques in Invertebrate Pathology: Application and Evaluation of Pathogens for Control of Insects and Other Invertebrate Pests Kluwer Academic Dordrecht 255–282
R. Han R.-U. Ehlers (1999) ArticleTitleTrans-specific nematicidal activity of Photorhabdus luminescens Nematology 1 687–693
K. Hu J. Li J.M. Webster (1999) ArticleTitleNematicidal metabolites produced by Photorhabdus luminescens (Enterobacterioaceae), bacterial symbiont of entomopathogenic nematodes Nematology 1 457–469 Occurrence Handle1:CAS:528:DC%2BD3cXivFKjsw%3D%3D
H.K. Kaya R. Gaugler (1993) ArticleTitleEntomopathogenic nematodes Annu. Rev. Entomol. 38 181–206
M.J. Kershaw E.R. Moorhouse R. Bateman S.E. Reynolds A.K. Charnley (1999) ArticleTitleThe role of destruxins in the pathogenicity of Metarhizium anisopliae for three species of insects J. Invertebr. Pathol. 74 213–223 Occurrence Handle1:CAS:528:DyaK1MXmslyit7k%3D Occurrence Handle10534408
A.M. Koppenhöfer H.K. Kaya (1997) ArticleTitleAdditive and synergistic interaction between entomopathogenic nematodes and Bacillus thuringiensis for scarab grub control Biol. Control 8 131–137
L.A. Lacey R. Frutos H.K. Kaya P. Vail (2001) ArticleTitleInsect pathogens as biological control agents: Do they have a future Biol. Control 21 230–248
J.G. Li Chen J.M. Webster (1997) ArticleTitleNematophin, a novel antimicrobial substance produced by Xenorhabdus nematophilus (Enterobacteriaceae) Can. J. Microbiol. 43 770–773 Occurrence Handle10.1139/m97-110
P.W. Maxwell G. Chen J.M. Webster G.B. Dunphy (1994) ArticleTitleStability and activities of antibiotics produced during infection of the insect Galleria mellonella by two isolates of Xenorhabdus nematophilus Appl. Environ. Microbiol. 60 715–721 Occurrence Handle1:CAS:528:DyaK2cXhsFygtL8%3D Occurrence Handle16349198
B.V. McInerney W.C. Taylor M.J. Lacey R.J. Akhurst R.P. Gregson (1991) ArticleTitleBiological active metabolites from Xenorhabdus spp. Part II: Benzopyran-1-one derivatives with gastroprotective activity J. Nat. Prod. 5 785–795
B.V. McInnerney R.P. Gregon M.J. Lacey R.J. Akhurst G.R. Lyons S.H. Rhodes R.J.D. Smith L.M. Engelhardt A.H. White (1991) ArticleTitleBiologically active metabolites from Xenorhabdus spp. Part 1. Dithiolopyrrolone derivatives with antibiotic activity J. Nat. Prod. 54 774–784
J. Singh J.L., Faull (1988) Antagonism and biological control. K.G Mukerji K.L. Garg (Eds) Biocontrol of Plant Disease CRC Press Boca Raton, FL 167–177
SPSS (Systat statistical software) 2002. statistical product and service solution, system user’s guide Version 11
H. Strasser A. Vey T.M. Butt (2000) ArticleTitleAre there any risks in using entomopathogenic fungi for pest control, with particular reference to the bioactive metabolites of Metarhizium, Tolypocladium and Beauveria species? Biocontrol Sci Technol. 10 717–735
A. Vey R. Hoagland T.M Butt (2001) Toxic metabolites of fungal biocontrol agents. T.M. Butt Jackson N. Magan (Eds) Fungal Biocontrol Agents: Progress Problem and Potential. CABI Wallingford 311–346
J.M., Webster G. Chen K. Hu J. Li (2002) Bacterial Metabolites. R. Gaugler (Eds) Entomopathogenic Nematology CAB International UK. 75–92
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Ansari, M.A., Tirry, L. & Moens, M. Antagonism between entomopathogenic fungi and bacterial symbionts of entomopathogenic nematodes. Biocontrol 50, 465–475 (2005). https://doi.org/10.1007/s10526-004-5524-4
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DOI: https://doi.org/10.1007/s10526-004-5524-4