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Identification of Isthmin1 in the small annual fish, Nothobranchius guentheri, as a novel biomarker of aging and its potential rejuvenation activity

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Abstract

Isthmin 1 (Ism1) has been shown to play roles in multiple biological processes including morphogenesis, hematopoiesis, antiviral immune response and suppression of tumor growth. However, it remains unknown if it plays any role in aging process. Here we showed for the first time that Ism1 was a new age-related biomarker, which decreased with age in fish, mice and humans. Interestingly, Ism1 was also useful to measure the “rejuvenated” age of fish Nothobranchius guentheri reversed by salidroside treatment and temperature reduction, providing additional evidence that Ism1 was an aging biomarker. In addition, we clearly showed that dietary intake of recombinant Ism1 had little effects on the body length and weight of aging N. guentheri, but it retarded the onset of age-related biomarkers and prolonged both the maximum and median lifespan of the fish. We also showed that Ism1 exerted its rejuvenation activity via the enhancement of antioxidant system. Collectively, our results indicate that Ism1 is not only is a novel biomarker of aging but also a potential rejuvenation factor capable of reversing aging of N. guentheri.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No: 32073000) and the Marine S&T Fund of Shandong province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (Grant No: 2018SDKJ0302-1).

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Authors and Affiliations

  1. Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China

    Congjun Li, Lili Song, Yang Zhou & Shicui Zhang

  2. Clinical Laboratory, Qingdao Municipal Hospital, Qingdao, 266011, Shandong, China

    Jiangshui Yuan

  3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266003, China

    Shicui Zhang

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  1. Congjun Li
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  2. Lili Song
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Correspondence to Shicui Zhang.

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Li, C., Song, L., Zhou, Y. et al. Identification of Isthmin1 in the small annual fish, Nothobranchius guentheri, as a novel biomarker of aging and its potential rejuvenation activity. Biogerontology 23, 99–114 (2022). https://doi.org/10.1007/s10522-021-09948-5

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