Abstract
A 60-day intervention study was conducted in which the participants took a low dose of a multivitamin and mineral supplement. The study consists of a final number of 66 volunteers (30 males and 36 females), divided into two age groups of 30–35 and 60–65 years. For 30 days they took a multivitamin and mineral supplement with 1× the recommended daily intake (RDI) followed by another 30 days with 2× the RDI. The aim of the study was to monitor oxidative stress and redox status of both young and old age groups. In serum, the expected increase of the water-soluble vitamins folate and vitamin B12 was observed with a concomitant decrease in homocysteine. Serum biomarkers of oxidative stress, the reactive oxygen metabolites, of the antioxidant status, the biological antioxidant potential did not change. However, the total thiol levels in serum, biomarker of the redox status, decreased significant, only in both groups of elderly after 60 days. In erythrocytes, there was a change in the glutathione metabolism as observed by an increase in glutathione reductase and to a lower extend in glutathione peroxidase, indicating an increase in oxidative stress in all groups. It is concluded that a low-dosed multivitamin and -mineral supplementation have different effects on the redox status in young versus old. It remained to explain why a low dose of a multivitamin and -mineral supplement cause increased oxidative stress.
Similar content being viewed by others
References
Aleksandrova K, Drogan D, Boeing H, Jenab M, Bueno-de-Mesquita HB, Jansen E et al (2014a) Adiposity, mediating biomarkers and risk of colon cancer in the European prospective investigation into cancer and nutrition study. Int J Cancer 134:612–621
Aleksandrova K, Jenab M, Bueno-de-Mesquita HB, Fedirko V, Kaaks R, Lukanova A, van Duijnhoven FJB, Jansen E et al (2014b) Biomarker patterns of inflammatory and metabolic pathways are associated with risk of colorectal cancer: results from the European Prospective Investigation into Cancer and Nutrition (EPIC). Eur J Epidemiol 29:261–275
Bjelakovic G, Nikolova D, Gluud LL, Simonetti RG, Gluud C (2008) Antioxidant supplements for prevention of mortality in healthy participants and patients with various diseases. Cochrane Database Syst Rev. doi:10.1002/14651858.CD007176
Bowry VW, Ingold KU, Stocker R (1992) Vitamin E in human low-density lipoprotein. When and how this antioxidant becomes a pro-oxidant. Biochem J 288:341–344
Breuer W, Hershko C, Cabantchik ZI (2000) The importance of non-transferrin bound iron in disorders of iron metabolism. Transfus Sci 23:85–192
Brewer GJ (2010) Epigenetic oxidative redox shift (EORS) theory of aging unifies the free radical and insulin signaling theories. Exp Gerontol 45:173–179
Buell JS, Arsenault LN, Scott TM, Qiu QW, Rosenberg IH et al (2007) Multivitamin use and B vitamin status in a homebound elderly population. J Nutr Health Aging 11:299–303
Castellani RJ, Moreira PI, Perry G, Zhu X (2012) The role of iron as a mediator of oxidative stress in Alzheimer disease. BioFactors 38:133–138
Fabian E, Bogner M, Kickinger A, Wagner KH, Elmadfa I (2012) Vitamin status in elderly people in relation to the use of nutritional supplements. J Nutr Health Aging 16:206–212
Hannon-Fletcher MP, Armstrong NC, Scott JM, Pentieva K, Bradbury I et al (2004) Determining bioavailability of food folates in a controlled intervention study. Am J Clin Nutr 80:911–918
Homocysteine Lowering Trialists’ Collaboration (1998) Lowering blood homocysteine with folic acid based supplements: meta-analysis of randomized trials. Brit Med J 316:894–898
Leufkens AM, van Duijnhoven FJB, Woudt SHS, Siersema PD, Jenab M, Jansen EHJM, Pischon T et al (2012) Biomarkers of oxidative stress and risk of developing colorectal cancer: a cohort-nested case-control study in the EPIC study. Amer J Epidemiol 175:653–663
McKay DL, Perrone G, Rasmussen H, Dallal G, Hartman W et al (2000) The effects of a multivitamin/mineral supplement on micronutrient status, antioxidant capacity and cytokine production in healthy older adults consuming a fortified diet. J Am Coll Nutr 19:613–621
Meneghini R (1997) Iron homeostasis, oxidative stress, and DNA damage. Free Rad Biol Med 23:783–792
Miller ER, Pastor-Barriuso R, Darshan Dalal D, Riemersma RA, Lawrence J, Appel LJ et al (2005) Meta-analysis: high-dosage vitamin e supplementation may increase all-cause mortality. Ann Intern Med 142:37–46
Park S, Johnson M, Fischer JG (2008) Vitamin and mineral supplements: barriers and challenges for older adults. J Nutr Elder 27:297–317
Ruskovska T, Jansen EHJM, Antarorov R (2014) Evaluation of assays for measurement of serum (anti)oxidants in hemodialysis patients. Biomed Res Int Article id 843157
Saum KU, Dieffenbach AK, Jansen EHJM, Holleczek B, Hauer K, Brenner H (2015) Association between oxidative stress and frailty in an elderly German population: results from the ESTHER cohort study. Gerontology. Accepted for publication
Sayin VI, Ibrahim MX, Larsson E, Nilsson JA, Lindahl P, Bergo MO (2014) Antioxidants accelerate lung cancer progression in mice. Sci Transl Med 6(221):ra15. doi:10.1126/scitranslmed.3007653
Schnorr CE, da Morrone Silva M, Simões-Pires A, da Bittencourt Silva L, Zeidán-Chuliá F, Fonseca Moreira JC (2014) Supplementation of adult rats with moderate amounts of β-carotene modulates the redox status in plasma without exerting pro-oxidant effects in the brain: a safer alternative to food fortification with vitamin A? Nutrients 6:5572–5582
Schöttker B, Saum KU, Jansen EHJM, Boffetta P, Trichopoulou A et al. (2014) Metabolic, inflammatory and oxidative stress markers of (multi-) morbidity at older age. A large population-based cohort study. Accepted by J Gerontology: Part A Biol Med Sci, doi:10.1093/gerona/glu111
Schumacher B, van der Pluijm I, Moorhouse MJ, Kosteas T, Robinson AR et al (2008) Delayed and accelerated aging share common longevity assurance mechanisms. PLoS Genet 4:e1000161
Thavanati PKR, Kanala KR, de Diosand AE, Garza JMC (2008) Age-related correlation between antioxidantenzymes and DNA damage with smoking andbody mass index. J Gerontol 63A:360–364
Traverso N, Ricciarelli R, Nitti M, Marengo B, Furfaro AL et al (2013) Role of glutathione in cancer progression and chemoresistance. Oxid Med Cell Longevity. doi:10.1155/2013/972913
Winterbourn CC (2013) The biological chemistry of hydrogen peroxide. Methods Enzymol 528:3–25
Acknowledgments
This study was performed within project SOR/340006 of the National Institute of Public Health and the Environment.
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jansen, E., Beekhof, P., Tamosiunas, A. et al. Biomarkers of oxidative stress and redox status in a short-term low-dosed multivitamin and mineral supplementation study in two human age groups. Biogerontology 16, 645–653 (2015). https://doi.org/10.1007/s10522-015-9568-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10522-015-9568-x