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Effect of age on cytokine response in an experimental model of osteomyelitis

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Abstract

To study the effect of age on cytokine response in an experimental model of osteomyelitis. Forty adult male Wistar rats received a stainless steel needle, intramedullarly in the left tibia. Young rats (3 months old) and old rats (22 months old) were allotted in: Group A: Sterile implant. Group B: Sterile implant + slime producing S. aureus. Rats were sacrificed 9 weeks after surgery. Determinations: Cytokines (ELISA) in blood and in tibia extract and the number of bacteria in tibia and implant. The Wilcoxon, Mann–Whitney U tests were used (P ≤ 0.01 significant). Infection was detected in every old rat receiving S. aureus, and in 7 of 10 young rats. In blood: prior to surgery, old rats presented higher IL-2 and lower IL-4 levels. Surgery alone did not induce significant changes in old rats; surgery + S. aureus induced significant increases of IL-2 and IL-10 in young rats, and of IL-6 in old rats. Tibia analysis S. aureus group showed increased levels of: IL-10 in young rats, and IL-1β in old rats. In experimentally induced osteomyelitis, significant differences were observed in cytokine response with regard to age.

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Acknowledgments

This work was supported by Grant SAF2004-03986 from the Ministerio de Educación y Ciencia de España-European Council.

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Authors and Affiliations

  1. Department of Surgery, Faculty of Medicine, University of Zaragoza, c/Domingo Miral s/n, 50009, Zaragoza, Spain

    Felícito García-Alvarez, Marta Navarro-Zorraquino, Angel Castro, Cristina Pastor & Ricardo Lozano

  2. Immunology, Health and Sport Sciences Faculty, Zaragoza University, Plaza Universidad 3, 22002, Huesca, Spain

    José María Grasa & Ana Martínez

  3. Department of Pathology, Veterinary Faculty, Zaragoza University, Miguel Servet 177, 50013, Zaragoza, Spain

    Marta Monzón

  4. Department of Medicine, Royo Villanova Hospital, Avda. San Gregorio 30, 50015, Zaragoza, Spain

    Ignacio García-Alvarez

  5. Department of Microbiology, Universitary Clinic Hospital “Lozano Blesa”, Avda. San Juán Bosco 14, 50009, Zaragoza, Spain

    Javier Castillo

  6. Felícito García-Alvarez García, c/Zumalacárregui 4, 4º Dcha., 50006, Zaragoza, Spain

    Felícito García-Alvarez

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  1. Felícito García-Alvarez
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  2. Marta Navarro-Zorraquino
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Correspondence to Felícito García-Alvarez.

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García-Alvarez, F., Navarro-Zorraquino, M., Castro, A. et al. Effect of age on cytokine response in an experimental model of osteomyelitis. Biogerontology 10, 649–658 (2009). https://doi.org/10.1007/s10522-008-9211-1

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