Skip to main content
SpringerLink
Log in

Dose-dependency of Life Span Prolongation of F344/DuCrj Rats Injected with (−)deprenyl

  • Research Article
  • Published:
Biogerontology Aims and scope Submit manuscript

Abstract

The effect of (−)deprenyl (D) on prolonging survival has previously been reported in different species of animals. In rats, three studies reported a positive effect, while one study reported a shortening of life spans. In the present study, we attempted to clarify past discrepancies in the results based on the speculation that there exists a certain effective dose range for this effect of the drug. F344/DuCrj rats of both sexes began to receive subcutaneous (s.c.) injections of D at the age of 18 months at a dose of 0.25 mg/kg/injection (inj.), 3 times a week. Control animals were given a vehicle (a saline solution). Average life spans of animals (days) were significantly increased in both male (895 ± 109.7, n=30; 967.8 ± 88.6, n=30, control vs. D treated, P<0.01, t-test) and female (924.7 ± 132.2, n=38; 987.1 ± 133.4, n=39, P<0.05) rats by 8.1% and 6.7%, respectively. We have previously reported that a dose of 0.5mg/kg/inj. (s.c.) significantly increased the life span of male F344 rats, while a dose of 1.0 mg/kg/inj. somewhat shortened the life span, although the difference was not statistically significant. The results of the present study coupled with our previous reports clearly indicate that a proper dose of D within a certain dose range can significantly increase the life span of animals of both sexes, but that a greater dose becomes less effective and may actually adversely affect the life span of rats. The presence of this effective dose range of D may explain discrepancies in the effect of D on life spans of animals previously reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CAT:

catalase

D:

(-)depreny

inj:

injection

s.c.:

subcutaneous

SOD:

superoxide dismutase

TMIG:

Tokyo Metropolitan Institute of Gerontology

References

  • PC Bickford CE Adams SJ Boyson P Curella GA Gerhardt C Heron GO Ivy AMLY Lin MP Murphy K Poth DR Wallace DA Young NR Zahniser GM Rose (1997) ArticleTitleLong-term treatment of male F344 rats with deprenyl: assessment of effects on longevity, behavior, and brain function Neurobiol Aging 18 309–318 Occurrence Handle10.1016/S0197-4580(97)80313-2 Occurrence Handle9263197 Occurrence Handle1:CAS:528:DyaK2sXls1Ont7s%3D

    Article  PubMed  CAS  Google Scholar 

  • EJ Calabrese LA Baldwin (2002) ArticleTitleDefining hormesis Hum Exp Toxicol 21 91–97 Occurrence Handle12102503 Occurrence Handle1:CAS:528:DC%2BD38XlsF2hsr4%3D

    PubMed  CAS  Google Scholar 

  • MC Carrillo S Kanai M Nokubo GO Ivy Y Sato K Kitani (1992) ArticleTitleDeprenyl increases activities of superoxide dismutase and catalase in striatum but not in hippocampus: the sex and age-related differences in the optimal dose in the rat Exp Neurol 116 286–294 Occurrence Handle10.1016/0014-4886(92)90008-E Occurrence Handle1587332 Occurrence Handle1:CAS:528:DyaK38Xks1arsro%3D

    Article  PubMed  CAS  Google Scholar 

  • MC Carrillo S Kanai Y Sato M Nokubo GO Ivy K Kitani (1993) ArticleTitleThe optimal dosage of (−)deprenyl for increasing superoxide dismutase activities in several brain regions decreases with age in male Fischer 344 rats Life Sci 52 1925–1934 Occurrence Handle10.1016/0024-3205(93)90633-E Occurrence Handle8505859 Occurrence Handle1:CAS:528:DyaK3sXks1aqs7k%3D

    Article  PubMed  CAS  Google Scholar 

  • MC Carrillo K Kitani S Kanai Y Sato K Miyasaka GO Ivy (1994) ArticleTitleThe effect of a long term (6 months) treatment with (−)deprenyl on antioxidant enzyme activities in selective brain regions in old female F-344 rats Biochem Pharmacol 47 1333–1338 Occurrence Handle10.1016/0006-2952(94)90331-X Occurrence Handle8185641 Occurrence Handle1:CAS:528:DyaK2cXjtFGksr8%3D

    Article  PubMed  CAS  Google Scholar 

  • MC Carrillo S Kanai K Kitani GO Ivy (2000) ArticleTitleA high dose of long term treatment with deprenyl loses its effect on antioxidant enzyme activities as well as on survivals of Fischer-344 rats Life Sci 67 2539–2548 Occurrence Handle11104356 Occurrence Handle1:CAS:528:DC%2BD3cXnslaisbk%3D

    PubMed  CAS  Google Scholar 

  • IM Gallagher A Clow V Glover (1998) ArticleTitleLong term administration of (−)deprenyl increases mortality in male Wistar rats J Neural Transm 52 IssueIDSuppl 315–320 Occurrence Handle1:CAS:528:DyaK1cXjtlSjtLs%3D

    CAS  Google Scholar 

  • IM Gallagher A Clow P Jenner V Glover (1999) ArticleTitleEffect of long-term administration of pergolide and (−)deprenyl on age related decline in hole board activity and antioxidant enzymes in rats Biogenic Amines 15 379–393 Occurrence Handle1:CAS:528:DyaK1MXksVSqurc%3D

    CAS  Google Scholar 

  • K Kitani S Kanai Y Sato M Ohta GO Ivy MC Carrillo (1993) ArticleTitleChronic treatment of (−)deprenyl prolongs the life span of male Fischer 344 rats: further evidence Life Sci 52 281–288 Occurrence Handle10.1016/0024-3205(93)90219-S Occurrence Handle8423709 Occurrence Handle1:CAS:528:DyaK3sXlt1eltA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • K Kitani S Kanai GO Ivy MC Carrillo (1998) ArticleTitleAssessing the effects of deprenyl on longevity and antioxidant defences in different animal models Ann NY Acad Sci 854 291–306 Occurrence Handle9928438 Occurrence Handle1:CAS:528:DyaK1MXjtVeitQ%3D%3D

    PubMed  CAS  Google Scholar 

  • K Kitani C Minami K Isobe K Maehara S Kanai GO Ivy MC Carrillo (2002a) ArticleTitleWhy (−)deprenyl prolongs survivals of experimental animals: increase of anti-oxidant enzymes in brain and other body tissues as well as mobilization of various humoral factors may lead to systemic anti-aging effects Mech Ageing Dev 123 1087–1100 Occurrence Handle1:CAS:528:DC%2BD38XktFOlsbk%3D

    CAS  Google Scholar 

  • K Kitani C Minami T Yamamoto S Kanai GO Ivy MC Carrillo (2002b) ArticleTitlePharmacological interventions in aging and age-associated disorders. Potentials of propargylamines for human use Ann New York Acad Sci 959 295–307 Occurrence Handle1:CAS:528:DC%2BD38XktFWqu78%3D

    CAS  Google Scholar 

  • J Knoll (1988) ArticleTitleThe striatal dopamine dependency of life span in male rats: longevity study with (−)deprenyl Mech Ageing Dev 46 237–262 Occurrence Handle10.1016/0047-6374(88)90128-5 Occurrence Handle3147347 Occurrence Handle1:CAS:528:DyaL1MXovVOgsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • NW Milgram RJ Racine P Nellis A Mendonca GO Ivy (1990) ArticleTitleMaintenance of l-deprenyl prolongs life in aged male rats Life Sci 47 415–420 Occurrence Handle10.1016/0024-3205(90)90299-7 Occurrence Handle2118586 Occurrence Handle1:CAS:528:DyaK3cXlsVGhtro%3D

    Article  PubMed  CAS  Google Scholar 

  • M Nokubo (1985) ArticleTitlePhysical–chemical and biochemical differences in liver plasma membranes in aging F-344 rats J Gerontol 40 409–414 Occurrence Handle2989354 Occurrence Handle1:CAS:528:DyaL2MXlslKisrg%3D

    PubMed  CAS  Google Scholar 

  • S ThyagaRajan J Meites SK Auadri (1995) ArticleTitleDeprenyl reinitiates estrous cycles, reduces serum prolactin, and decreases the incidence of mammary and pituitary tumors in old acyclic rats Endocrinology 136 1103–1110 Occurrence Handle10.1210/en.136.3.1103 Occurrence Handle7867565 Occurrence Handle1:CAS:528:DyaK2MXjvFCjtrc%3D

    Article  PubMed  CAS  Google Scholar 

  • S ThyagaRajan SY Felten DL Felten (1998) ArticleTitleAntitumor effect of l-deprenyl in rats with carcinogen-induced mammary tumors Cancer Lett 123 177–183 Occurrence Handle10.1016/S0304-3835(97)00431-X Occurrence Handle9489486 Occurrence Handle1:CAS:528:DyaK1cXnt12gsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • S ThyagaRajan SK Quadri (1999) ArticleTitlel-deprenyl inhibits tumor growth, reduces serum prolactin, and suppresses brain monoamine metabolism in rats with carcinogen-induced mammary tumors Endocrine 10 225–232 Occurrence Handle10484286 Occurrence Handle1:CAS:528:DyaK1MXlt1Olsrc%3D

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Longevity Sciences, 36-3, Gengo, Moriokacho, Obu-shi, Tokyo, Japan

    K. Kitani

  2. Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan

    S. Kanai & K. Miyasaka

  3. Institute for Experimental Physiology, University of Rosario, Rosario, Argentina

    M. C. Carrillo

  4. University of Toronto, Scarborough, Canada

    G. O. Ivy

Authors
  1. K. Kitani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Kanai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Miyasaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. C. Carrillo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. O. Ivy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Kitani.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kitani, K., Kanai, S., Miyasaka, K. et al. Dose-dependency of Life Span Prolongation of F344/DuCrj Rats Injected with (−)deprenyl. Biogerontology 6, 297–302 (2005). https://doi.org/10.1007/s10522-005-4804-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10522-005-4804-4

Keywords

Search

Navigation