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Application of Impedance Spectroscopy for the Control of the Integrity of In Vitro Models of Barrier Tissues

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Bulletin of Experimental Biology and Medicine Aims and scope

We compared available methods for monitoring the integrity of in vitro models of barrier tissues and studied the possibility of using impedance spectroscopy to solve this problem. It was demonstrated (theoretically and experimentally) that TEER measurements are not sufficiently sensitive to detect small defects in the cell barrier that significantly affect its permeability. For obtaining reliable results, it is necessary to set a sufficiently high threshold TEER, which leads to the loss of many intact samples. At the same time, impedance spectroscopy has all advantages of the classical method of measuring TEER (it is rapid and non-invasive method), while its application in combination with the methods of machine learning allows reliable detection of defects in the cell barrier.

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Authors and Affiliations

  1. BioClinicum Research Center, Ministry of Health of the Russian Federation, Obninsk, Russia

    S. V. Nikulin, T. N. Gerasimenko, S. A. Shilin, G. S. Zakharova, I. N. Gazizov & D. A. Sakharov

  2. Moscow Institute of Physics and Technology (MIPT), Moscow, Russia

    S. V. Nikulin

  3. National Research Center of Radiology, Ministry of Health of the Russian Federation, Obninsk, Russia

    A. A. Poloznikov

Authors
  1. S. V. Nikulin
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  2. T. N. Gerasimenko
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  3. S. A. Shilin
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  4. G. S. Zakharova
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  5. I. N. Gazizov
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  6. A. A. Poloznikov
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  7. D. A. Sakharov
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Corresponding author

Correspondence to S. V. Nikulin.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 166, No. 10, pp. 512-517, October, 2018

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Nikulin, S.V., Gerasimenko, T.N., Shilin, S.A. et al. Application of Impedance Spectroscopy for the Control of the Integrity of In Vitro Models of Barrier Tissues. Bull Exp Biol Med 166, 512–516 (2019). https://doi.org/10.1007/s10517-019-04384-5

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  • DOI: https://doi.org/10.1007/s10517-019-04384-5

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