We studied combined effect of β-hairpin antimicrobial peptide tachyplesin I and cytotoxic agent cisplatin on tumor and normal human cell lines. MTT assay and flow cytometry showed that tachyplesin I selectively sensitized cancer cells to cisplatin in specified concentration ratios. In vitro experiments demonstrated that combined use of tachyplesin I and cisplatin allows decreasing the effective dose of the cytostatic thus reducing nonspecific toxicity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baguley BC. Multiple drug resistance mechanisms in cancer. Mol. Biotechnol. 2010; 46(3):308-316.
Chen J, Xu XM, Underhill CB, Yang S, Wang L, Chen Y, Hong S, Creswell K, Zhang L. Tachyplesin activates the classic complement pathway to kill tumor cells. Cancer Res. 2005; 65 (11):4614-4622.
Heiduschka G, Bigenzahn J, Brunner M, Thurnher D. Resveratrol synergistically enhances the effect of etoposide in HNSCC cell lines. Acta Otolaryngol. 2014; 134 (10):1071-1078.
Holstein SA, Hohl RJ. Synergistic interaction of lovastatin and paclitaxel in human cancer cells. Mol. Cancer Ther. 2001; 1(2):141-149.
Hui L, Leung K, Chen HM. The combined effects of antibacterial peptide cecropin A and anti-cancer agents on leukemia cells. Anticancer Res. 2002; 22(5):2811-2816.
Jamieson ER, Lippard SJ. Structure, recognition, and processing of cisplatin-DNA adducts. Chem. Rev. 1999; 99(9):2467-2498.
Kuzmin DV, Emelianova AA, Kalashnikova MB, Panteleev PV, Balandin SV, Serebrovskaya EO, Belogurova-Ovchinnikova OYu, Ovchinnikova TV. Сomparative in vitro study on cytotoxicity of recombinant b-hairpin peptides. Chem. Biol. Drug Des. 2017. doi: https://doi.org/10.1111/cbdd.13081.
Motawi TM, Sadik NA, Fahim SA, Shouman SA. Combination of imatinib and clotrimazole enhances cell growth inhibition in T47D breast cancer cells. Chem. Biol. Interact. 2015;233:147-156.
Ouyang GL, Li QF, Peng XX, Liu QR, Hong SG. Effects of tachyplesin on proliferation and differentiation of human hepatocellular carcinoma SMMC-7721 cells. World J. Gastroenterol. 2002;8(6):1053-1058.
Panteleev PV, Ovchinnikova TV. Improved strategy for recombinant production and purification of antimicrobial peptide tachyplesin I and its analogs with high cell selectivity. Biotechnol. Appl. Biochem. 2017;64(1):35-42.
Paredes-Gamero EJ, Martins MN, Cappabianco FA, Ide JS, Miranda A. Characterization of dual effects induced by antimicrobial peptides: regulated cell death or membrane disruption. Biochim. Biophys. Acta. 2012;1820(7):1062-1072.
Sreenivasan S, Krishnakumar S. Synergistic effect of curcumin in combination with anticancer agents in human retinoblastoma cancer cell lines. Curr. Eye Res. 2015;40(11):1153-1165.
Teixeira SF, Guimarães Idos S, Madeira KP, Daltoé RD, Silva IV, Rangel LB. Metformin synergistically enhances antiproliferative effects of cisplatin and etoposide in NCI-H460 human lung cancer cells. J. Bras. Pneumol. 2013;39(6):644-649.
Zhang HT, Wu J, Zhang HF, Zhu QF. Efflux of potassium ion is an important reason of HL-60 cells apoptosis induced by tachyplesin. Acta Pharmacol. Sin. 2006;27(10):1367-1374.
Zhu H, Luo H, Zhang W, Shen Z, Hu X, Zhu X. Molecular mechanisms of cisplatin resistance in cervical cancer. Drug Des. Devel. Ther. 2016;10:1885-1895.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 2, pp. 183-187, February, 2018
Rights and permissions
About this article
Cite this article
Kuzmin, D.V., Emel’yanova, A.A., Kalashnikova, M.B. et al. In Vitro Study of Antitumor Effect of Antimicrobial Peptide Tachyplesin I in Combination with Cisplatin. Bull Exp Biol Med 165, 220–224 (2018). https://doi.org/10.1007/s10517-018-4134-6
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-018-4134-6