We studied the effects of a concentrate of metabolites of Trichoderma harzianum Rifai F-180, an active producer of L-lysine-α-oxidase, and homogenous enzyme on a highly virulent bacteria Erwinia amylovora. The producer of antitumor and antiviral Trichoderma enzyme L-lysine-α-oxidase was cultured on a processing system of G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino). Activity of L-lysine-α-oxidase in the prepared concentrate of metabolic products of the producer was 5.4 U/ml, and activity of the homogenous enzyme was 50 U/ml. Antibacterial activity of the enzyme was shown in our experiments.
Similar content being viewed by others
References
Smirnova IP, Larichev VF. Patent RF No. 2473689. Tick-borne encephalitis virus inhibitor. Bull. No. 3. Published January 27, 2013.
Smirnova IP, Rakovskaja IV. Patent RF No. 2569150. Mycoplasma (Mycoplasma hominis) inhibitor producer. Bull. No. 32. Published November 20, 2015.
Smirnova IP, Alekseev SB, Shevchenko AA. Biosynthesis of L-Lysine-α-Oxidase, an Antitumor Enzyme, by Trichoderma spp. Antibiotiki Khimioter. 2009;54(5-6):8-12. Russian.
Benítez T. Biofungicides: Trichoderma as a biocontrol agent against phytopathogenic fungi. Recent research developments in microbiology. Trivandrum, 1998. Vol. 2. P. 129-150.
Limón MC, Chacón MR, Mejías R, Delgado-Jarana J, Rincón AM, Codón AC, Benítez T. Increased antifungal and chitinase specific activities of Trichoderma harzianum CECT 2413 by addition of a cellulose binding domain. Appl. Microbiol. Biotechnol. 2004;64(5):675-685.
Schaad NW, Jones J.B, Chun W. Laboratory Guide for Identification of Plant Pathogenic Bacteria. St. Paul, 2001. P. 373.
Shneider YA, Smirnova IP, Karimova EV. Inhibition of tobacco ringspot virus by the culture fluid of L-lysine-a-oxidase producing strain. Bull. Exp. Biol. Med. 2016;162(1):79-81.
Smirnova IP, Kuznetsova O.M, Ivanova-Radkevich VI, Orlova VS, Podboronov VM, Alexeev AA. Testing of an antitumor enzyme L-lysine-A-oxidase from Trichoderma Harzianum Rifai F-180. World J. Med. Sci. 2014;11(2):233-236.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 6, pp. 743-745, June, 2017
Rights and permissions
About this article
Cite this article
Smirnova, I.P., Karimova, E.V. & Shneider, Y.A. Antibacterial Activity of L-Lysine-α-Oxidase from the Trichoderma. Bull Exp Biol Med 163, 777–779 (2017). https://doi.org/10.1007/s10517-017-3901-0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-017-3901-0