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Antibacterial Activity of L-Lysine-α-Oxidase from the Trichoderma

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We studied the effects of a concentrate of metabolites of Trichoderma harzianum Rifai F-180, an active producer of L-lysine-α-oxidase, and homogenous enzyme on a highly virulent bacteria Erwinia amylovora. The producer of antitumor and antiviral Trichoderma enzyme L-lysine-α-oxidase was cultured on a processing system of G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (Pushchino). Activity of L-lysine-α-oxidase in the prepared concentrate of metabolic products of the producer was 5.4 U/ml, and activity of the homogenous enzyme was 50 U/ml. Antibacterial activity of the enzyme was shown in our experiments.

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References

  1. Smirnova IP, Larichev VF. Patent RF No. 2473689. Tick-borne encephalitis virus inhibitor. Bull. No. 3. Published January 27, 2013.

  2. Smirnova IP, Rakovskaja IV. Patent RF No. 2569150. Mycoplasma (Mycoplasma hominis) inhibitor producer. Bull. No. 32. Published November 20, 2015.

  3. Smirnova IP, Alekseev SB, Shevchenko AA. Biosynthesis of L-Lysine-α-Oxidase, an Antitumor Enzyme, by Trichoderma spp. Antibiotiki Khimioter. 2009;54(5-6):8-12. Russian.

  4. Benítez T. Biofungicides: Trichoderma as a biocontrol agent against phytopathogenic fungi. Recent research developments in microbiology. Trivandrum, 1998. Vol. 2. P. 129-150.

  5. Limón MC, Chacón MR, Mejías R, Delgado-Jarana J, Rincón AM, Codón AC, Benítez T. Increased antifungal and chitinase specific activities of Trichoderma harzianum CECT 2413 by addition of a cellulose binding domain. Appl. Microbiol. Biotechnol. 2004;64(5):675-685.

    Article  PubMed  Google Scholar 

  6. Schaad NW, Jones J.B, Chun W. Laboratory Guide for Identification of Plant Pathogenic Bacteria. St. Paul, 2001. P. 373.

  7. Shneider YA, Smirnova IP, Karimova EV. Inhibition of tobacco ringspot virus by the culture fluid of L-lysine-a-oxidase producing strain. Bull. Exp. Biol. Med. 2016;162(1):79-81.

    Article  CAS  PubMed  Google Scholar 

  8. Smirnova IP, Kuznetsova O.M, Ivanova-Radkevich VI, Orlova VS, Podboronov VM, Alexeev AA. Testing of an antitumor enzyme L-lysine-A-oxidase from Trichoderma Harzianum Rifai F-180. World J. Med. Sci. 2014;11(2):233-236.

    Google Scholar 

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Correspondence to I. P. Smirnova.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 6, pp. 743-745, June, 2017

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Smirnova, I.P., Karimova, E.V. & Shneider, Y.A. Antibacterial Activity of L-Lysine-α-Oxidase from the Trichoderma. Bull Exp Biol Med 163, 777–779 (2017). https://doi.org/10.1007/s10517-017-3901-0

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  • DOI: https://doi.org/10.1007/s10517-017-3901-0

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