We studied the properties of a tissue-engineered trachea consisting of a polyethylene terephthalate scaffold populated with autologous bone marrow mononuclear cells. The tissue-engineered constructs were obtained before surgery, during the postoperative period, and during autopsy. Cytomorphological analysis during the postoperative period showed the presence of mesenchymal stem cells on the inner surface of the implant on day 3 after surgery and cells of the respiratory epithelium on day 10-14. In autopsy samples, single epithelial cells, endothelial cells, and basal cells were found. Biocompatibility of the tissue-engineered trachea with autologous mononuclear cells of the patient was demonstrated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gilevich IV, Fedorenko TV, Pashkova IA, Porkhanov VA, Chekhonin VP. Effects of growth factors on mobilization of mesenchymal stem cells. Bull. Exp. Biol. Med. 2017;162(5):684-686.
Shishatskaya EI, Nikolaeva ED, Shumilova AA, Shabanov AV, Volova TG. Cultivation of multipotent mesenchymal bone marrow cells on matrixes made of resorbable Bioplastotan. Geny Kletki. 2013;8(1):57-65. Russian.
Crowley C, Birchall M, Seifalian A.M. Trachea transplantation: from laboratory to patient. Tissue Eng. Regen. Med. 2015;9(4):357-367.
Dorati R, Colonna C, Tomasi C, Genta I, Bruni G, Conti B. Design of 3D scaffolds for tissue engineering testing a tough polylactide-based graft copolymer. Mater. Sci. Eng. C Mater. Biol. Appl. 2014;34:130-139.
Doss AE, Dunn SS, Kucera KA, Clemson LA, Zwischenberger J. B. Tracheal replacements: part 2. ASAIO J. 2007;53(5):631-639.
Hertegard S. Tissue engineering in the larynx and airway. Curr. Opin. Otolaryngol. Head Neck Surg. 2016;24(6):469-476.
Jungebluth P, Haag JC, Lim ML, Lemon G, Sjöqvist S, Gustafsson Y, Ajalloueian F, Gilevich I, Simonson OE, Grinnemo KH, Corbascio M, Baiguera S, Del Gaudio C, Strömblad S, Macchiarini P. Verification of cell viability in bioengineered tissues and organs before clinical transplantation. Biomaterials. 2013;34(16):4057-4067.
Kumar PA, Hu Y, Yamamoto Y, Hoe NB, Wei TS, Mu D, Sun Y, Joo LS, Dagher R, Zielonka EM, Wang de Y, Lim B, Chow VT, Crum CP, Xian W, McKeon F. Distal airway stem cells yield alveoli in vitro and during lung regeneration following H1N1 influenza infection. Cell. 2011;147(3):525-538.
Musa M, Ponnuraj KT, Mohamad D, Rahman IA. Genotoxicity evaluation of dental restoration nanocomposite using comet assay and chromosome aberration test. Nanotechnology. 2013;24(1):ID 015105. 10.1088/0957-4484/24/1/015105.
O’Brien FJ. Biomaterials and scaffolds for tissue engineering. Materials Today. 2011;14(3):88-95.
Sheikh FA, Ju HW, Moon BM, Lee OJ, Kim JH, Park HJ, Kim DW, Kim DK, Jang JE, Khang G, Park CH. Hybrid scaffolds based on PLGA and silk for bone tissue engineering. J. Tissue Eng. Regen. Med. 2016;10(3):209-221.
Wormald JC, Fishman JM, Juniat S, Tolley N, Birchall MA. Regenerative medicine in otorhinolaryngology. J. Laryngol. Otol. 2015;129(8):732-739.
Wu W, Feng X, Mao T, Feng X, Ouyang HW, Zhao G, Chen F. Engineering of human tracheal tissue with collagen-enforced poly-lactic-glycolic acid non-woven mesh: a preliminary study in nude mice. Br. J. Oral Maxillofac. 2007;45(4):272-278.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 3, pp. 388-392, March, 2017
Rights and permissions
About this article
Cite this article
Gilevich, I.V., Polyakov, I.S., Porkhanov, V.A. et al. Morphological Analysis of Biocompatibility of Autologous Bone Marrow Mononuclear Cells with Synthetic Polyethylene Terephthalate Scaffold. Bull Exp Biol Med 163, 400–404 (2017). https://doi.org/10.1007/s10517-017-3813-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-017-3813-z