The cholinergic, GABAergic, and catecholaminergic neurons derived from mouse embryonic stem cells in a culture medium supplemented with recombinant NGF were phenotyped and scored. NGF stimulated generation of neurons, but not neuronal progenitors from embryonic stem cells and affected the proportion of specific types of neurons in cultures of differentiating embryonic stem cells. These findings open vista to employ NGF for generation of specific neuron types from embryonic stem cells for fundamental and toxicological studies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Antonov SA, Dolotov OV, Manuilova ES, Kobylyanskii AG, Kostrov SV, Safina DR, Khaidarova NV, Grivennikov IA. Isolation of mouse embryonic stem cells with inducible expression of human nerve growth factor.Vestn. Biotekhnol. Fiz.-Khim. Biol. 2012;8(52):5-12. Russian.
Aloe L, Rocco ML, Bianchi P, Manni L. Nerve growth factor: from the early discoveries to the potential clinical use. J. Transl Med. 2012;10:239.
Brodski C, Schaubmar A, Dechant G. Opposing functions of GDNF and NGF in the development of cholinergic and noradrenergic sympathetic neurons. Mol. Cell. Neurosci. 2002;19(4):528-538.
Cai C, Grabel L. Directing the differentiation of embryonic stem cells to neural stem cells. Dev. Dyn. 2007;236(12):3255-3266.
Cattaneo E, McKay R. Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor. Nature. 1990;347:762-765.
Levenberg S, Burdick JA, Kraehenbuehl T, Langer R. Neurotrophin-induced differentiation of human embryonic stem cells on three-dimensional polymeric scaffolds. Tissue Eng. 2005;11(3-4):506-512.
Manni L, Rocco ML, Bianchi P, Soligo M, Guaragna M, Barbaro SP, Aloe L. Nerve growth factor: basic studies and possible therapeutic applications. Growth Factors. 2013;31(4):115-122.
Nilbratt M, Porras O, Marutle A, Hovatta O, Nordberg A. Neurotrophic factors promote cholinergic differentiation in human embryonic stemcell-derived neurons. J. Cell. Mol. Med. 2010;14(6B):1476-1484.
Schnitzler AC, Mellott TJ, Lopez-Coviella I, Tallini YN, Kotlikoff MI, Follettie MT, Blusztajn JK. BMP9 (bone morphogenetic protein 9) induces NGF as an autocrine/paracrine cholinergic trophic factor in developing basal forebrain neurons. J. Neurosci. 2010;30(24):8221-8228.
Schuldiner M, Eiges R, Eden A, Yanuka O, Itskovitz-Eldor J, Goldstein RS, Benvenisty N. Induced neuronal differentiation of human embryonic stem cells. Brain Res. 2001;913(2):201-205.
Skaper SD. The biology of neurotrophins, signalling pathways, and functional peptide mimeticsof neurotrophins and their receptors. CNS Neurol. Disord. Drug Targets. 2008;7(1):46-62.
Solozobova V, Wyvekens N, Pruszak J. Lessons from the embryonic neural stem cell niche for neural lineage differentiation of pluripotent stem cells. Stem Cell Rev. 2012;8(3):813-829.
Tomellini E, Lagadec C, Polakowska R, Le Bourhis X. Role of p75 neurotrophin receptor in stem cell biology: more than just a marker. Cell. Mol. Life Sci. 2014;71(13):2467-2481.
Weitzer G. Embryonic stem cell-derived embryoid bodies: an in vitro model of eutherian pregastrulation development and early gastrulation. Handb. Exp. Pharmacol. 2006;(174):21-51.
Wobus AM, Grosse R, Schöneich J. Specific effects of nerve growth factor on the differentiation pattern of mouse embryonic stem cells in vitro. Biomed. Biochim. Acta. 1988;47(12):965-973.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 11, pp. 636-640, November, 2016
Rights and permissions
About this article
Cite this article
Antonov, S.A., Manuilova, E.S., Dolotov, O.V. et al. Effect of Nerve Growth Factor on Neural Differentiation of Mouse Embryonic Stem Cells. Bull Exp Biol Med 162, 679–683 (2017). https://doi.org/10.1007/s10517-017-3686-1
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-017-3686-1