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Effects of Release-Active Antibodies to CD4 Receptor on the Level of lck-Kinase in Cultured Mononuclear Cells from Human Peripheral Blood

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For evaluation of effects of release-active antibodies to CD4 on cultured lymphocytes from human peripheral blood, we measured intracellular content of lck-kinase cell-based ELISA. In cells treated with release-active antibodies to CD4, the content of intracellular lck-kinase significantly (p<0.01) decreased in comparison with the control (purified water processed in a similar way). Phytohemagglutinin had no effect on the concentration of lck-kinase in cells. The decrease in the content of CD4-associated lck protein suggests that the preparation enhanced intracellular coupling of lck-kinase with T-cell receptor and potentiated T-cell immune response.

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Authors and Affiliations

  1. 1Laboratory of Physiologically Active Substances, Department of Molecular and Cellular Pathophysiology, Research Institute of General Pathology and Pathophysiology, Moscow, Russia

    A. G. Emel’yanova, N. V. Petrova, S. G. Morozov & O. I. Epshtein

  2. Research and Production Company Materia Medica Holding, Moscow, Russia

    V. V. Grechenko, E. A. Gorbunov & S. A. Tarasov

  3. State Research Centre Institute of Immunology, Federal Medical-Biological Agency of the Russian Federation, Moscow, Russia

    I. P. Shilovskii & M. R. Khaitov

Authors
  1. A. G. Emel’yanova
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  2. V. V. Grechenko
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  3. N. V. Petrova
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  4. I. P. Shilovskii
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  5. E. A. Gorbunov
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  6. S. A. Tarasov
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  7. M. R. Khaitov
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  8. S. G. Morozov
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  9. O. I. Epshtein
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Corresponding author

Correspondence to A. G. Emel’yanova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 9, pp. 304-307, September, 2016

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Emel’yanova, A.G., Grechenko, V.V., Petrova, N.V. et al. Effects of Release-Active Antibodies to CD4 Receptor on the Level of lck-Kinase in Cultured Mononuclear Cells from Human Peripheral Blood. Bull Exp Biol Med 162, 323–326 (2017). https://doi.org/10.1007/s10517-017-3606-4

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  • DOI: https://doi.org/10.1007/s10517-017-3606-4

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