Skip to main content

Advertisement

SpringerLink
Log in

Design of Polyepitope DNA Vaccine against Breast Carcinoma Cells and Analysis of Its Expression in Dendritic Cells

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

An Erratum to this article was published on 01 March 2016

Polyepitope DNA vaccine inducing T-cell-mediated immune response against cancer-specific antigens is a promising tool for selective elimination of tumor cells. Breast cancer-specific polyepitope DNA vaccine was designed using TEpredict and PolyCTLDesigner software on the basis of immunogenic peptides of HER2 and Mammaglobin-1 (Mam) tumor antigens. LPS-free preparations of plasmid DNA encoding polyepitope T-cell antigen and full-length copies of HER2 and Mam antigens were obtained. TaqMan-PCR systems for evaluation of the expression of immunogens in cells were created. The protocol of vaccine DNA delivery into dendritic cells was optimized. Expression of the target immunogens in dendritic cells derived from human peripheral blood mononuclear fraction after transfection with plasmid DNA preparations is demonstrated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. P. Laktionov, A. A. Ilyichev, A. A. Bondar’, et al., Patent RF No. 2408729. A Method for Obtaining Plasmid DNA from Bacterial Cells, Byull., No. 1, January 10, 2011.

  2. D. V. Antonets and S. I. Bazhan, BMC Res. Notes, 6, 4-7 (2013).

    Article  Google Scholar 

  3. S. I. Bazhan, L. I. Karpenko, T. N. Ilyicheva, et al., Mol. Immunol., 47, No. 7-8, 1507-1515 (2010).

    Article  CAS  PubMed  Google Scholar 

  4. A. Bonehill, C. Heirman, S. Tuyaerts, et al., J. Immunol., 172, No. 11, 6649-6657 (2004).

    Article  CAS  PubMed  Google Scholar 

  5. Y. Z. Chen, G. X. Ruan, X. L. Yao, et al., Pharm. Res., 30, No. 6, 1502-1512 (2013).

    Article  CAS  PubMed  Google Scholar 

  6. J. Ferlay, H. Shin, F. Bray, et al., Int. J. Cancer, 127, No. 12, 2893-2917 (2010).

    Article  CAS  PubMed  Google Scholar 

  7. M. Ladjemi, W. Jacot, T. Chardes, et al., Cancer Immunol. Immunother., 59, No. 9, 1295-1312 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. S. G. Li and L. Li, Biomed. Rep., 1, No. 4, 499-505 (2013).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. K. J. Livak and T. Schmittgen, Methods, 25, No. 4, 402-408 (2001).

    Article  CAS  PubMed  Google Scholar 

  10. C. Lundegaard, O. Lund, S. Buus, et al., Immunology, 130, No. 3, 309-318 (2010).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. R. B. Mailliard, A. Wankowicz-Kalinska, Q. Cao, et al., Cancer Res., 64, No. 17, 5934-5937 (2004).

    Article  CAS  PubMed  Google Scholar 

  12. A. Milani, D. Sangiolo, F. Montemurro, et al., Ann. Oncol., 24, No. 7, 1740-1748 (2013).

    Article  CAS  PubMed  Google Scholar 

  13. V. Tiriveedhi, T. P. Fleming, P. S. Goedegebuure, et al., Breast Cancer Res. Treat., 138, No. 1, 109-118 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. A. Villalobos, M. Welch, and J. Minshull, Methods Mol. Biol., 852, 197-213 (2012).

    Article  CAS  PubMed  Google Scholar 

  15. L. Zhang, K. Udaka, H. Mamitsuka, et al., Brief Bioinform., 13, No. 3, 350-364 (2012).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Chemical Biology and Basic Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia

    Zh. K. Nazarkina, M. V. Khar’kova, V. V. Vlasov, A. A. Ilyichev & P. P. Laktionov

  2. Vector State Research Center of Virology and Biotechnology, Koltsovo, Russia

    D. V. Antonets, E. S. Morozkin, S. I. Bazhan, L. I. Karpenko, A. A. Ilyichev & P. P. Laktionov

  3. E. N. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Novosibirsk, Russia

    Zh. K. Nazarkina

Authors
  1. Zh. K. Nazarkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. V. Khar’kova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. V. Antonets
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. S. Morozkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. I. Bazhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. I. Karpenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. V. V. Vlasov
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. A. Ilyichev
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. P. P. Laktionov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zh. K. Nazarkina.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 160, No. 10, pp. 492-496, October, 2015

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nazarkina, Z.K., Khar’kova, M.V., Antonets, D.V. et al. Design of Polyepitope DNA Vaccine against Breast Carcinoma Cells and Analysis of Its Expression in Dendritic Cells. Bull Exp Biol Med 160, 486–490 (2016). https://doi.org/10.1007/s10517-016-3203-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-016-3203-y

Key Words

Search

Navigation