Skip to main content
SpringerLink
Log in

Comparative Analysis of the Expression of Neural Stem Cell-Associated Genes during Neocortex and Retina Development in Human

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We compared the expression of Sox2, Oct4, Nanog, Pax6, Prox1 genes associated with plasticity of neural stem and progenitor cells during human neocortex and retina development and in cell cultures. At the analyzed stages of neurogenesis, Pax6 gene is expressed in the neocortex and retina at constant levels, the expression is by one order of magnitude higher in the retina. The dynamics of Sox2 and Pax6 expression in the neocortex was similar. The expression of Oct4 and Nanog genes during neurogenesis in the neocortex and human fetal retina reflects the existence of a high-plasticity cell pool. The dynamics of βIII-tubulin expression indicates that the retina develops more rapidly than the neocortex. Our experiments showed that genetically determined cell potencies typical of native cells are realized in primary cultures without specific stimulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. B. I. Verdiev, R. A. Poltavtsev, O. V. Podgornyi, et al., Kletoch. Tekhnol. Biol. Med., No. 4, 206–214 (2009).

  2. M. Bani-Yaghoub, R. G. Tremblay, J. X. Lei, et al., Dev. Biol., 295, No. 1, 52–66 (2006).

    Article  PubMed  CAS  Google Scholar 

  3. R. Catena, C. Tiveron, A. Ronchi et al., J. Biol. Chem., 279, No. 40, 41,846-41,857 (2004).

    Article  CAS  Google Scholar 

  4. L. Du, G. Lin, and G. Lu, Zhong Nan Da Xue Xue Bao Yi Xue Ban, 34, No. 12, 1157–1165 (2009).

    PubMed  CAS  Google Scholar 

  5. J. B. Kim, B. Greber, M. J. Arauzo-Bravo, et al., Nature, 461, 649–653 (2009).

    Article  PubMed  CAS  Google Scholar 

  6. H. Klassen, B. Ziaeian, I. I. Kirov, et al., J. Neurosci. Res., 77, No. 3, 334–343 (2004).

    Article  PubMed  CAS  Google Scholar 

  7. A. Lavado and G. Oliver, Dev. Dyn., 236, No. 2, 518–524 (2007).

    Article  PubMed  CAS  Google Scholar 

  8. M. Manuel and D. J. Price, Brain Res. Bull., 66, Nos. 4–6, 387–393 (2005).

    Article  PubMed  CAS  Google Scholar 

  9. F. T. Merkle and A. Alvarez-Buylla, Cur. Opin. Cell. Biol., 18, No. 6, 704–709 (2006).

    Article  CAS  Google Scholar 

  10. Z. Mo and N. Zecevic, Cereb. Cortex., 18, No. 6, 1455–1465 (2008).

    Article  PubMed  Google Scholar 

  11. T. Okuda, K. Tagawa, M. L. Qi, et al., Brain Res. Mol. Brain Res. 132, No. 1, 18–30 (2004).

    Article  PubMed  CAS  Google Scholar 

  12. V. Ramos-Mejia, D. Escalante-Alcalde, T. Kunath, et al., Dev. Dyn., 232, No. 1, 180–190 (2005).

    Article  PubMed  CAS  Google Scholar 

  13. S. N. Sansom, D. S. Griffiths, A. Faedo, et al., PLoS Genet., 5, No. 6, e1000511 (2009).

    Article  PubMed  Google Scholar 

  14. G. M. Seigel, A. S. Hackam, A. Ganguly, et al., Mol. Vis., 13, 823–832 (2007).

    PubMed  CAS  Google Scholar 

  15. M. Takeshi, T. Morito, Y. Kenji, et al., Stem Cells, 30, No. 6, 1109–1119 (2012).

    Article  Google Scholar 

  16. O. V. Taranova, S. T. Magness, B. M. Fagan, et al., Genes Dev., 20, No. 9, 1187–1202 (2006).

    Article  PubMed  CAS  Google Scholar 

  17. S. Thomas, M. Thomas, P. Wincker, et al., Hum. Mol. Genet., 17, No. 21, 3411–3425 (2008).

    Article  PubMed  CAS  Google Scholar 

  18. M. S. Tsai, S. M. Hwang, Y. L. Tsai, et al., Biol. Reprod., 74, No. 3, 545–551 (2006).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. N. K. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia

    B. I. Verdiev, L. A. Milyushina, O. V. Podgornyi, R. D. Zinov’eva & M. A. Aleksandrova

  2. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health and Social Development of the Russian Federation, Moscow, Russia

    R. A. Poltavtseva & G. T. Sukhikh

Authors
  1. B. I. Verdiev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. A. Milyushina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. V. Podgornyi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. A. Poltavtseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. D. Zinov’eva
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. T. Sukhikh
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. A. Aleksandrova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. I. Verdiev.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 183–191, October, 2012

Rights and permissions

Reprints and permissions

About this article

Cite this article

Verdiev, B.I., Milyushina, L.A., Podgornyi, O.V. et al. Comparative Analysis of the Expression of Neural Stem Cell-Associated Genes during Neocortex and Retina Development in Human. Bull Exp Biol Med 154, 529–536 (2013). https://doi.org/10.1007/s10517-013-1994-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-013-1994-7

Key Words

Search

Navigation