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Effects of HSP27 Chaperone on THP-1 Tumor Cell Apoptosis

  • Oncology
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Bulletin of Experimental Biology and Medicine Aims and scope

The role of Hsp27 (heat shock protein 27) chaperone in regulation of THP-1 tumor cell apoptosis was studied. Realization of tumor cell apoptosis under conditions of in vitro culturing with Hsp27 specifi c inhibitor (KRIBB3) was evaluated by fluorescent microscopy with FITC-labeled annexin V and propidium iodide. Measurements of Bcl-2 family proteins (Bcl-2, Bax, Bad) in tumor cells incubated with Hsp27 inhibitor were carried out by Western blotting. Chaperone Hsp27 acted as apoptosis inhibitor in THP-1 tumor cells modulating the proportion of antiapoptotic (Bcl-2) and proapoptotic (Bax and Bad) proteins.

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Authors and Affiliations

  1. Department of Fundamental Clinical Medicine, Research and Educational Center of Molecular Medicine, Department of Pathophysiology, Siberian State Medical University, the Ministry of Health and Social Development of the Russian Federation, Tomsk, Russia

    E. V. Kaigorodova, N. V. Ryazantseva, V. V. Novitskii, A. N. Maroshkina & M. V. Belkina

Authors
  1. E. V. Kaigorodova
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  2. N. V. Ryazantseva
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  3. V. V. Novitskii
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  4. A. N. Maroshkina
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  5. M. V. Belkina
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Correspondence to E. V. Kaigorodova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 154, No. 7, pp. 87-90, July, 2012

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Kaigorodova, E.V., Ryazantseva, N.V., Novitskii, V.V. et al. Effects of HSP27 Chaperone on THP-1 Tumor Cell Apoptosis. Bull Exp Biol Med 154, 77–79 (2012). https://doi.org/10.1007/s10517-012-1879-1

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  • DOI: https://doi.org/10.1007/s10517-012-1879-1

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