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Fluorescent Methods in the Study of UV-Induced Changes in Structural and Functional State of Human Blood Lymphocytes

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Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m2 was studied by methods of laser flow cytofluorometry, indirect immunofluorescence, and fluorescent probes. Using a combination of these methods, we have showed that UV light in the specified doses induced changes in the surface phenotype of T cells: stimulation or suppression of the expression of antigen-recognizing receptor complex molecules (CD3, CD4, and CD8 markers) and their redistribution on the surface of immunocompetent cells (capping effect) with the formation of receptor clusters of various types.

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Authors and Affiliations

  1. Voronezh State University, Voronezh, Russia

    V. G. Artyukhov, O. V. Putintseva & V. A. Vdovina

  2. N. N. Burdenko Voronezh State Medical Academy, Voronezh, Russia

    M. V. Pashkov & D. V. Vasilenko

Authors
  1. V. G. Artyukhov
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  2. O. V. Putintseva
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  3. V. A. Vdovina
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  4. M. V. Pashkov
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  5. D. V. Vasilenko
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Corresponding author

Correspondence to V. G. Artyukhov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 153, No. 6, pp. 891-895, June, 2012

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Artyukhov, V.G., Putintseva, O.V., Vdovina, V.A. et al. Fluorescent Methods in the Study of UV-Induced Changes in Structural and Functional State of Human Blood Lymphocytes. Bull Exp Biol Med 153, 908–912 (2012). https://doi.org/10.1007/s10517-012-1856-8

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  • DOI: https://doi.org/10.1007/s10517-012-1856-8

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