The concentration of O2 during coculturing practically did not affect the subpopulation composition of T lymphocytes (CD3+/CD4+, CD3+/CD8+, CD3+/CD16+/CD56+ T cells) under conditions of PHA-induced activation. Coculturing with mesenchymal stromal cells (MSC) led to a significant decrease in the ratio of lymphocytes carrying activation markers (CD3+/CD25+ and CD3+/HLA-DR+) and increase in the number of CD3+/CD16+/CD56+ T cells. The percent of activated HLA-DR+ T cells in a heterotypic culture with MSC at 5% O2 was much lower than that observed under normal conditions of culturing (20% O2). Our results suggest that antigen presentation by T lymphocytes due to HLA-DR expression can be reduced in the target tissues at low concentration of O2, while the interaction between allogeneic MSC probably contributes to more significant inhibition of the immune response.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 151, No. 3, pp. 319–321, March, 2011
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Buravkova, L.B., Grigorieva, O.G., Andreeva, E.R. et al. Subpopulation Composition and Activation of T Lymphocytes during Coculturing with Mesenchymal Stromal Cells in Medium with Different O2 Content. Bull Exp Biol Med 151, 344–346 (2011). https://doi.org/10.1007/s10517-011-1326-8
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DOI: https://doi.org/10.1007/s10517-011-1326-8