Abstract
The spin probe method was employed to study in vitro the effect of regulatory peptide thyroliberin on structural state of surface (0.8 nm) and deep (2 nm) lipid layers of the plasma membranes in mouse liver and brain. Thyroliberin in a concentration range of 10−3–10−18 M enhanced structural order of surface lipids, the maximum effect was observed at 10−9–10−10 M. The dose-effect dependencies for microviscosity of deep lipids were nonlinear and had 3 extrema at 10−4–10−7 M, 10−9 M, and 10−14–10−16 M. The greatest changes in lipid microviscosity produced by 10−9 M thyroliberin are explained by lipid-receptor interaction.
Similar content being viewed by others
References
I. P. Ashmarin, L. M. Asanova, K. R. Abbasova, et al., Radiats. Biol. Radioecol., 43, No. 3, 324–327 (2003).
I. P. Ashmarin, A. P. Kulashev, and S. A. Chepurnov, Fiziol. Zh., 75, No. 5, 627–632 (1989).
E. B. Burlakova, A. A. Konradov, and E. L. Mal’tseva, Khim. Fiz., 22, 21–40 (2003).
V. E. Zhernovkov, N. G. Bogdanova, and N. P. Pal’mina, Biol. Membr., 22, No. 5, 388–395 (2005).
V. E. Zhernovkov, T. V. Lelekova, and N. P. Pal’mina, Radiats. Biol. Radioecol., 43, No. 3, 331–333 (2003).
A. N. Kuznetsov, Spin Probe Method [in Russian], Moscow (1976).
A. S. Polezina, K. A. Anikienko, and V. K. Kurochkin, Ross. Khim. Zh., 43, No. 5, 72–79 (1999).
J. Cao, D. O’Donnell, H. Vu, et al., J. Biol. Chem., 273, No. 48, 32,281–32,287 (1998).
M. C. Gershengorn and R. Osman, Endocrinology, 142, No. 1, 2–10 (2001).
E. G. Loten and J. C. Redshaw-Loten, Anal. Biochem., 154, No. 1, 183–185 (1986).
O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem., 193, No. 1, 265–275 (1951).
W. Paschen, Cell Calcium, 34, Nos. 4–5, 365–383 (2003).
A. A. Spector and M. A. Yorek, J. Lipid Res., 26, No. 9, 1015–1035 (1985).
Y. Sun, X. Lu, and M. C. Gershengorn, J. Mol. Endocrinol., 30, No. 2, 87–97 (2003).
V. P. Whittaker, Meth. Neurochem., N.Y. 2, 1–52 (1972).
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 8, pp. 151–154, August, 2007
Rights and permissions
About this article
Cite this article
Zhernovkov, V.E., Pal’mina, N.P. In vitro effects of thyroliberin on structural state of plasma membranes in mouse brain and liver. Bull Exp Biol Med 144, 185–187 (2007). https://doi.org/10.1007/s10517-007-0285-6
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10517-007-0285-6