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Immobilization and long-term culturing of mouse embryonic stem cells in collagen-chitosan gel matrix

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

We propose a method of creation of a 3D matrix consisting of native collagen fibers and natural polysaccharide chitosan. The collagen-chitosan hydrogels maintain viability and prolipherative activity of embryonic stem cells obtained from internal cells of mouse blastocyst. The proposed system forming hydrogels in situ can be used in cell therapy for immobilization and targeted delivery of stem cells.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino

    I. I. Selezneva, I. V. Savintseva, E. F. Vikhlyantseva, G. A. Davydova & B. K. Gavrilyuk

Authors
  1. I. I. Selezneva
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  2. I. V. Savintseva
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  3. E. F. Vikhlyantseva
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  4. G. A. Davydova
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  5. B. K. Gavrilyuk
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Correspondence to I. I. Selezneva.

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Translated from Kletochnye Tekhnologii v Biologii i Medicine, No. 3, pp. 135–139, August, 2006

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Selezneva, I.I., Savintseva, I.V., Vikhlyantseva, E.F. et al. Immobilization and long-term culturing of mouse embryonic stem cells in collagen-chitosan gel matrix. Bull Exp Biol Med 142, 119–122 (2006). https://doi.org/10.1007/s10517-006-0308-8

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  • DOI: https://doi.org/10.1007/s10517-006-0308-8

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