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Interaction of rifabutin with model membranes

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Abstract

Liposomal and free rifabutin were separated by the method of gel filtration. The percents of rifabutin bound to liposomes of different phospholipid composition were measured. The presence of negatively charged phospholipids increased the degree of binding. Binding decreased with increasing the ionic strength. Incubation of rifabutin with liposomes containing anthryl phosphatidylcholine was accompanied by fluorescence quenching. Activity of rifabutin depended on the phospholipid composition of liposomes. Our results indicate that binding of rifabutin is associated with electrostatic and hydrophobic interactions.

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References

  1. O. V. Bogomolov, A. P. Kaplun, and V. I. Shvets, Bioorgan. Khim., 10, No. 11, 1559–1564 (1984).

    Google Scholar 

  2. R. Gennis, Biological Membranes. Molecular Structure and Functions [in Russian], Moscow (1997).

  3. A. S. Minina, G. M. Sorokoumova, A. A. Selishcheva, et al., Biofizika, 49, No. 4, 674–679 (2004).

    CAS  PubMed  Google Scholar 

  4. G. M. Sorokoumova, A. A. Selishcheva, O. P. Tyurina, et al., Ibid., 47, No. 2, 268–276 (2002).

    CAS  PubMed  Google Scholar 

  5. A. A. Tsybanev and G. B. Sokolova, Antibiot. Khimioter., 44, No. 8, 30–36 (1999).

    CAS  PubMed  Google Scholar 

  6. Yu. A. Shchipunov, Usp. Khim., 66, No. 4, 3228–3252 (1997).

    Google Scholar 

  7. M. M. Gaspar, S. Neves, F. Portaels, et al., Antimicrob. Agents Chemother., 44, No. 9, 2424–2430 (2000).

    Article  CAS  PubMed  Google Scholar 

  8. R. R. C. New, Liposomes as Tools in Basic Research and Industry, Eds. J. R. Philippot and F. Schuber, Boca Raton (1995), pp. 3–20.

  9. N. Rastogi, K. S. Goh, M. Berchel, and A. Bryskier, J. Antimicrob. Chemother., 46, 565–570 (2000).

    Article  CAS  PubMed  Google Scholar 

  10. H. Saito, K. Sato, and H. Tomioka, Tubercle, 69, No. 3, 187–192 (1988).

    Article  CAS  PubMed  Google Scholar 

  11. A. Wanger and K. Mills, J. Clin. Microbiol., 34, No. 7, 1672–1676 (1996).

    CAS  PubMed  Google Scholar 

Download references

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Correspondence to A. A. Selishcheva.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 140, No. 12, pp. 664–666, December, 2005

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Shakina, Y.N., Vostrikov, V.V., Sorokoumova, G.M. et al. Interaction of rifabutin with model membranes. Bull Exp Biol Med 140, 711–713 (2005). https://doi.org/10.1007/s10517-006-0062-y

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  • DOI: https://doi.org/10.1007/s10517-006-0062-y

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