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Low occurrence of WSSV in Penaeus monodon nauplii and post-larvae produced from PCR-negative broodstocks

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Abstract

White spot syndrome virus (WSSV) is a devastating, infectious virus affecting farming tiger shrimp (Penaeus monodon). In efforts to limit trans-boundary movement of this pathogen and reduce the economic and socioeconomic impact of disease in shrimp culture, there is considerable scope for more effective use of DNA-based methods of pathogen detection. To assess the prevalence of the WSSV, broods, nauplii and post-larvae (PL) samples were collected from nine shrimp hatcheries located in the southeast and southwest regions of Bangladesh during the period from February 2008 to May 2014 and subjected to screening for WSSV by polymerase chain reaction (PCR) technique. The prevalence of WSSV during the 7-year period ranged from 48.15 to 62.99 %, 12.88 to 31.55 % and 4.03 to 8.89 % in broodstocks, nauplii and post-larvae, respectively. Out of a total of 5489 brood samples analyzed during the 7 years, 2,977 were found to be PCR positive for WSSV giving an overall prevalence of 54.24 %. The overall rates of PCR positive were 19.52 % for nauplii and 6.25 % for PL. The rates of infection in the nauplii and PL were much less than that observed in the broodstocks as the nauplii and PL samples were collected from broods found to be negative for WSSV by PCR. A follow-up study showed successful culture in about 87 % of the shrimp farms stocked with the PL that were PCR negative for WSSV. This study confirmed that PCR is an effective diagnostic tool for the screening of broodstocks before being used for spawning in the hatchery, to prevent vertical transmission of WSSV from parents to larvae. However, to avail the advantage of PCR screening for a risk-free crop, sources of potential horizontal contaminations should also be controlled.

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Abbreviations

DNA:

Deoxyribonucleic acid

PCR:

Polymerase chain reaction

WSSV:

White spot syndrome virus

PL:

Post-larvae

MT:

Metric ton

References

  • Ayub F, Sarker MY, Alam MS (2008) Prevalence of white spot syndrome virus infection detected by one-step and nested PCR in selected tiger shrimp (Penaeus monodon) hatcheries. Aquac Int 16:405–415

    Article  CAS  Google Scholar 

  • Chang P, Wang CLY, Kou GH (1996) Identification of white spot syndrome associated baculovirus (WSBV) target organs in the shrimp Penaeus monodon by in situ hybridization. Dis Aquat Org 27:131–139

    Article  Google Scholar 

  • Chou HY, Huang CY, Wang CH, Chiang HC, Lo CF (1995) Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis Aquat Org 23:165–173

    Article  Google Scholar 

  • Debnath P, Karim M, Belton B (2014) Comparative study of the reproductive performance and white spot syndrome virus (WSSV) status of black tiger shrimp (Penaeus monodon) collected from Bay of Bengal. Aquaculture 424–425:71–77

    Article  Google Scholar 

  • Escobedo-Bonilla CM, Alday-Sanz V, Wille M, Sorgeloos P, Pensaert MB, Nauwynck HJ (2008) A review on the morphology, molecular characterization, morphogenesis and pathogenesis of white spot syndrome virus. J Fish Dis 31:1–18

    Article  CAS  PubMed  Google Scholar 

  • Flegel TW (1997) Special topic review: major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World J Microbiol Biotechnol 13:433–442

    Article  Google Scholar 

  • Flegel TW (2006) Detection of major penaeid shrimp viruses in Asia, an historical perspective with emphasis on Thailand. Aquaculture 258:1–33

    Article  Google Scholar 

  • FRSS (2013) Fisheries statistical yearbook of Bangladesh, vol 29. Fisheries Resources Survey System (FRSS), Department of fisheries, Bangladesh

  • Hoa TTT, Zwart MP, Phuong NT, Vlak JM, de Jong MCM (2011) Transmission of white spot syndrome virus in improved-extensive and semi-intensive shrimp production systems: a molecular epidemiology study. Aquaculture 313:7–14

    Article  Google Scholar 

  • Hossain MS, Chakraborty A, Joseph B, Otta SK, Karunasagar I, Karunasagar I (2001) Detection of new hosts for white spot syndrome virus of shrimp using nested polymerase chain reaction. Aquaculture 198:1–11

    Article  Google Scholar 

  • Hsu HC, Lo CF, Liu KF, Su MS, Kou GH (1999) Studies on effective PCR screening strategies for white spot syndrome virus (WSSV) detection in Penaeus monodon brooders. Dis Aquat Org 39:13–19

    Article  CAS  PubMed  Google Scholar 

  • Kanchanaphum P, Wongteerasupaya C, Sitidilokratana N, Boonsaeng Y, Panyim S, Tassanakajon A, Withyachumnarnkul B, Flegel TW (1998) Experimental transmission of white spot syndrome virus (WSSV) from crabs to shrimp Penaeus monodon. Dis Aquat Org 11:1–7

    Article  Google Scholar 

  • Karim M, Sarwer RH, Brooks AC, Gregory R, Jahan ME, Belton B (2011) The incidence of suspected white spot syndrome virus in semi-intensive and extensive shrimp farms in Bangladesh: implications for management. Aquac Res 43:1357–1371

    Article  Google Scholar 

  • Karunasagar I, Otta SK, Karunasagar I (1997) Histopathological and bacteriological study of white spot syndrome of Penaeus monodon along the west coast of India. Aquaculture 153:9–13

    Article  Google Scholar 

  • Khadijah S, Neo SY, Hossain MS, Miller LD, Mathavan S, Kwang J (2003) Identification of white spot syndrome virus latency-related genes in specific-pathogenfree shrimps by use of a microarray. J Virol 77:10162–10167

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kim CK, Kim PK, Sohn SG, Sim DS, Park MA, Heo MS, Lee TH, Lee JD (1998) Development of a polymerase chain reaction (PCR) procedure for the detection of baculovirus associated with white spot syndrome (WSSV) in penaeid shrimp. J Fish Dis 21:11–17

    Article  CAS  Google Scholar 

  • Kimura T, Yamano K, Nakano H, Momoyama K, Hiraoka M, Inouye K (1996) Detection of penaeid rod-shaped DNA virus (PRDV) by PCR. Fish Pathol 31:93–98

    Article  CAS  Google Scholar 

  • Larkins PE (1995) Report on disease problems of cultured brackish water shrimp and freshwater prawns in Bangladesh, vol 1. Bangladesh Second Aquaculture Development Project. MOFL/DOF, Government of Bangladesh, p 134

  • Lightner DV (1996) Viral diseases. In: McVey A (ed) A handbook of shrimp pathology and diagnostic procedures for disease of cultured penaeid shrimp, vol 13. World Aquaculture Society, Baton Rouge, pp 1–72

    Google Scholar 

  • Lo CF, Leu JH, Ho CH, Chen CH, Peng SE, Chen YT, Chou CM, Yeh PY, Huang CJ, Chou HY, Wang CH, Kou GH (1996a) Detection of baculovirus associated with white spot syndrome (WSSV) in penaeid shrimps using polymerase chain reaction. Dis Aquat Org 25:133–141

    Article  CAS  Google Scholar 

  • Lo CF, Ho CH, Peng CH, Hsu HC, Chu YL, Chang CF, Liu KF, Su MS et al (1996b) White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Dis Aquat Org 27:212–225

    Article  Google Scholar 

  • Lo CF, Ho CH, Chen CH, Liu KF, Chiu YL, Yeh PY, Peng SE, Hsu HC (1997) Detection and tissue tropism of white spot syndrome baculovirus (WSSV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Dis Aquat Organ 30:53–72

    Article  Google Scholar 

  • Maeda M, Itami T, Furumoto A, Hennig O, Imamura T, Kondo M, Hirono I, Aoki T, Takahashi Y (1998) Detection of penaeid rod-shaped DNA virus (PRDV) in wild-caught shrimp and other crustaceans. Fish Pathol 33(4):373–380

    Article  CAS  Google Scholar 

  • Magbanua FO, Natividad KT, Migo VP, Alfafara CG, de la Pena FO, Miranda RO, Albaladejo JD, Nadala EC Jr et al (2000) White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines. Dis Aquat Org 10:77–82

    Article  Google Scholar 

  • Marks H, Mennens M, Vlak JM, van Hulten MCW (2003) Transcriptional analysis of the white spot syndrome virus major virion protein genes. J Gen Virol 84:1517–1523

    Article  CAS  PubMed  Google Scholar 

  • Mayo MA (2002) A summary of taxonomic changes recently approved by ICTV. Arch Virol 147:1655–1656

    Article  CAS  PubMed  Google Scholar 

  • Momoyama K, Hiraoka M, Nakano H, Koube H, Inouye K, Oseka N (1994) Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: histopathological study. Fish Pathol 29:141–148

    Article  Google Scholar 

  • Muegue MFS, Geduspan JS, Caipang CMA (2013) Optimization of PCR protocols for the detection of viral pathogens in shrimp aquaculture in the Philippines. Eur J Exp Biol 3(6):270–275

    CAS  Google Scholar 

  • Oidtmann B, Stentiford GD (2011) Whitespot syndrome virus (WSSV) concentrations in Crustacean tissues—a review of data relevant to assess the risk associated with commodity trade. Transbound Emerg Dis. doi:10.1111/j.1865-1682.2011.01231.x

    Google Scholar 

  • OIE (2003) Manual of diagnostic tests for aquatic animals, 4th edn. Office International des Epizootics, Paris 358

    Google Scholar 

  • Otta SK, Shuba G, Joseph B, Chakraborthy A, Karunasagar I, Karunasagar I (1999) Polymerase chain reaction (PCR) detection of white spot syndrome virus (WSSV) in cultured and wild crustaceans in India. Dis Aquat Org 38:67–70

    Article  Google Scholar 

  • Peinado-Guevara LI, López-Meyer M (2006) Detailed monitoring of white spot syndrome virus (WSSV) in shrimp commercial ponds in Sinaloa, Mexico by nested PCR. Aquaculture 251:33–45

    Article  CAS  Google Scholar 

  • Peng SE, Lo CF, Lin SC, Chen LL, Chang YS, Liu KF, Su MS, Kou GH (2001) Performance of WSSV infected and WSSV-negative Penaeus monodon post larvae in culture ponds. Dis Aquat Org 46:165–172

    Article  CAS  PubMed  Google Scholar 

  • Rajan PR, Ramasamy P, Purushothaman V, Brennan GP (2000) Whitespot baculovirus syndrome in the Indian shrimp Penaeus monodon and P. indicus. Aquaculture 184:31–44

    Article  Google Scholar 

  • Rajendran KV, Makesh M, Karunasagar I (2012) Monodon Baculovirus of shrimp. Indian J Virol 23(2):149–160

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • van Hulten MC, Witteveldt J, Peters S, Kloosterboer N, Tarchini R, Fiers M, Sandbrink H, Lankhorst RK, Vlak JM (2001) The white spot syndrome virus DNA genome sequence. Virology 286:7–22

    Article  PubMed  Google Scholar 

  • Vaseeharan B, Jayakumar R, Ramasamy P (2003) PCR-based detection of white spot syndrome virus in cultured and captured crustaceans in India. Lett Appl Microbiol 37:443–447

    Article  CAS  PubMed  Google Scholar 

  • Wang CH, Lo CF, Chang PS, Kou GH (1998) Experimental infection of white spot baculovirus in some cultured and wild decapods in Taiwan. Aquaculture 164:221–231

    Article  Google Scholar 

  • Wongteerasupaya C, Vickers JE, Sriurairatana S, Nash GL, Akarajamorn A, Boonsaeng V, Panyim S, Tassanakajon A, Withyachumnarkul B, Flegel TW (1995) A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Dis Aquat Org 21:69–77

    Article  Google Scholar 

Download references

Acknowledgments

We thank Mr. M. A. Hasan Panna the owner of the Pranti PCR Laboratory, Cox’s Bazar, for his generosity in providing us fund with places for laboratory use. We are grateful to the hatchery owners who allowed us to collect the samples and Mr. Milon, Technician, Pranti PCR Laboratory for his cooperation in laboratory analysis. The staffs of Gazi Shrimp Farm Ltd, Khulna are acknowledged for their valuable and long-term help with field work. We also thank the owners of shrimp farms for their kind cooperation in this study.

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Authors and Affiliations

  1. Department of Aquatic Resource Management, Faculty of Fisheries, Sylhet Agricultural University, Sylhet, 3100, Bangladesh

    Sabuj Kanti Mazumder

  2. Marine Ecosystem Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, DE, Malaysia

    Mazlan Abd. Ghaffar & Simon Kumar Das

  3. Institute of Tropical Aquaculture, University of Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia

    Mazlan Abd. Ghaffar

  4. School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, DE, Malaysia

    Sabuj Kanti Mazumder & Simon Kumar Das

  5. Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Md. Samsul Alam

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  1. Sabuj Kanti Mazumder
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Correspondence to Sabuj Kanti Mazumder.

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Mazumder, S.K., Ghaffar, M.A., Das, S.K. et al. Low occurrence of WSSV in Penaeus monodon nauplii and post-larvae produced from PCR-negative broodstocks. Aquacult Int 23, 1109–1123 (2015). https://doi.org/10.1007/s10499-014-9868-1

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  • DOI: https://doi.org/10.1007/s10499-014-9868-1

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