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Immune responses and protection in rohu vaccinated against Aeromonas hydrophila infection

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Abstract

In this study, efficacy of three antigenic preparations from the fish pathogen Aeromonas hydrophila was evaluated as vaccine candidate in rohu Labeo rohita. The rabbit anti-rohu globulin conjugate was prepared in rabbit using sera obtained from healthy adult rohu. Three antigens, namely formalin inactivated A. hydrophila (FAH), FAH mixed with Freund’s incomplete adjuvant in a 1:1 (v/v) ratio (FAH + A), and extra cellular product were prepared. Protection of rohu against A. hydrophila infection was tested at days 10, 20, and 30 post-vaccination. At the end of the vaccination, fish were challenged with A. hydrophila (dose: 1 × 107 cfu ml−1) and relative per cent of survival was recorded up to 60th day of post-challenge. Study of cellular immunological parameters including antigen-specific leucocyte proliferation, antigen-specific nitric oxide production, and superoxide anion production exhibited significantly higher (p < 0.05) values on 10th and 20th day of post-vaccination compared with control. Among all the antigenic groups, FAH + A showed most significant responses compared with the other groups and produced maximum antibody on 30th day following vaccination. Among the humoral immune responses, lysozyme activity showed almost similar trend like cellular parameters. The result of the challenge study showed a higher level of survival in all the vaccinated groups, especially in FAH + A group. Our results suggest that vaccine FAH + A can effectively protect rohu against A. hydrophila infection and could offer an appropriate strategy to prevent this infection in rohu farms.

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References

  • Alexander JB, Ingram GA (1992) Noncellular non-specific defence mechanisms of fish. Ann Rev Fish Dis 2:249–279

    Article  Google Scholar 

  • Amend DF (1981) Potency testing of fish vaccines. International symposium on fish biologics: serodiagnostics and vaccines. Dev Biol Stand 49:447–454

    Google Scholar 

  • APHA, Awwa, WEF (1998) Standard methods for the examination of water and waste water, 20th edn. American Public Health Association, American Water Works Association, Water Environment Association, Washington, pp 413–426

    Google Scholar 

  • Azad IS, Shankar KM, Mohan CV, Kalita B (1999) Biofilm vaccine of Aeromonas hydrophila—standardization of dose and duration for oral vaccination of carps. Fish Shellfish Immunol 9:519–528

    Article  Google Scholar 

  • Azad IS, Shankar KM, Mohan CV, Kalitav B (2000) Uptake and processing of biofilm and free-cell vaccines of Aeromonas hydrophila in Indian major carps and common carp following oral vaccination-antigen localization by a monoclonal antibody. Dis Aquat Organ 43:103–108

    Article  CAS  PubMed  Google Scholar 

  • Bastardo A, Ravelo C, Castro N et al (2012) Effectiveness of bivalent vaccines against Aeromonas hydrophila and Lactococcus garvieae infections in rainbow trout Oncorhynchus mykiss (Walbaum). Fish Shellfish Immunol 32:756–761

    Article  CAS  PubMed  Google Scholar 

  • Behera T, Nanda PK, Mohanty C, Mohapatra D, Swain P et al (2010) Parenteral immunization of fish, Labeo rohita with Poly d, l-lactide-co-glycolic acid (PLGA) encapsulated antigen microparticles promotes innate and adaptive immune responses. Fish Shellfish Immunol 28:320–325

    Article  CAS  PubMed  Google Scholar 

  • Bondad-Reantaso MG, Suba singhe RP, Arthur JR et al (2005) Disease and health management in Asian aquaculture. Vet Parasitol 132:249–272

    Article  PubMed  Google Scholar 

  • Chandran MR, Aruna BV, Logambal SM, Michael RD (2002) Immunisation of Indian major carps against Aeromonas hydrophila by intraperitoneal injection. Fish Shellfish Immunol 13:1–9

    Article  CAS  PubMed  Google Scholar 

  • CIFA (2004) Annual report Central Institute of Freshwater Aquaculture, Bhubaneswar

  • Das P, Joardar SN, Abraham TJ, Kamilya D, Batabyal S (2009) Dynamic changes in immune-effector characteristics of Indian major carp, rohu (Labeo rohita) sensitized with Aeromonas hydrophila. Indian J Comp Microbiol Immunol Infect Dis 30:45–49

    Google Scholar 

  • Dash S, Das SK, Samal J, Ojha PK, Patra JK, Thatoi H (2011) Dose dependence specific and non-specific immune responses of Indian major carp (L. rohita Ham) to intraperitoneal injection of formalin killed Aeromonas hydrophila whole cell vaccine. Vet Res Commun 35:541–552

    Article  PubMed  Google Scholar 

  • Di Giulio RT, Habig C, Gallagher EP (1993) Effects of black rock harbor sediments on indices of biotransformation, oxidative stress, and DNA integrity in channel catfish. Aquat Toxicol 26:1–22

    Article  Google Scholar 

  • Diamond RD, Krzesicki R, Jao W (1978) Damage to pseudohyphal forms of Candida albicans by neutrophils in the absence of serum in vitro. J Clin Invest 61:349–359

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ellis AE (1990) Lysozyme assay. In: Stolen JS, Fletcher EC, Aderson DP, Robetrson DS, van Muiswinkel WB (eds) Techniques in fish immunology. SOS publications, USA, pp 101–103

    Google Scholar 

  • Fridovich I (1995) Superoxide radical and superoxide dismutases. Ann Rev Biochem 64:97–112

    Article  CAS  PubMed  Google Scholar 

  • Giri SS, Sukumaran V, Oviya M (2013) Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish Shellfish Immunol 34:660–666

    Article  CAS  PubMed  Google Scholar 

  • Green IC, Wagner DA, Glogowski J, Skipper PL et al (1982) Analysis of nitrate, nitrite and (15 N) nitrate in biological fluids. Anal Biochem 126:131–138

    Article  CAS  PubMed  Google Scholar 

  • Gross KA, Powell MD, Butler R, Morrison RN, Nowak BF (2005) Changes in the innate immune response of Atlantic salmon, Salmo salar L., exposed to experimental infection with Neoparamoeba sp. J Fish Dis 28:293–299

    Article  CAS  PubMed  Google Scholar 

  • Gudding R, Lillehaug A, Evensen O (1999) Recent development in fish vaccinology. Vet Immunol Immunopathol 72:203–212

    Article  CAS  PubMed  Google Scholar 

  • Hastings TS (1988) Furunculosis vaccines. In: Ellis AE (ed) Fish vaccination. Academic Press, New York, pp 93–111

    Google Scholar 

  • John MB, Chandran MR, Aruna BV, Anbarasu K (2002) Production of superoxide anion by head kidney leukocytes of Indian major carps immunised with bacterins of Aeromonas hydrophila. Fish Shellfish Immunol 12:201–207

    Article  CAS  PubMed  Google Scholar 

  • Kamilya D, Maiti TK, Joardar SN, Mal BC (2006) Adjuvant effect of mushroom glucan and bovine lactoferrin upon Aeromonas hydrophila vaccination in catla, Catla catla (Hamilton). J Fish Dis 29:331–337

    Article  CAS  PubMed  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–273

    CAS  PubMed  Google Scholar 

  • Lund V, Jorgensen T, Holm KO, Eggest G (1991) Humoral immune response in Atlantic salmon, Salmo salar L., to cellular and extracellular antigens of Aeromonas salmonicida. J Fish Dis 14:443–452

    Article  Google Scholar 

  • Marsden MJ, Secombes CJ (1997) The influence of vaccine preparations on the induction of antigen specific responsiveness in rainbow trout, Oncorhynchus mykiss. Fish Shellfish Immunol 7:455–469

    Article  Google Scholar 

  • Midtlyng PJ, Reitan LJ, Lillehaug A, Ramstad A (1996) Protection, immune responses and side effects in Atlantic salmon (Salmo salar) vaccinated against furunculosis by different procedures. Fish Shellfish Immunol 6:599–613

    Article  Google Scholar 

  • Murray CK, Fletcher TCF (1976) The immunohistochemical localization of lysozyme in plaice, Pleuronectes platessa, tissues. J Fish Biol 9:324–329

    Article  Google Scholar 

  • Saikia D, Kamilya D (2012) Immune responses and protection in catla (Catla catla) vaccinated against epizootic ulcerative syndrome. Fish Shellfish Immunol 32:353–359

    Article  CAS  PubMed  Google Scholar 

  • Secombes CJ (1990) Isolation of salmonid macrophages and analysis of their killing activity. In: Stolen JS, Fletcher TC, Anderson DP, Robertson BS, van Muiswinkel WB (eds) Techniques in fish immunology, vol I. SOS Publications, Fair Haven, pp 137–154

    Google Scholar 

  • Swain P, Behura A, Dash S, Nayak SK (2007) Serum antibody response of Indian major carp, Labeo rohita to three species of pathogenic bacteria; Aeromonas hydrophila, Edwardsiella tarda and Pseudomonas fluorescens. Vet Immunol Immunopathol 117:137–141

    Article  CAS  PubMed  Google Scholar 

  • Yin Z, Lam TJ, Sin YM (1997) Cytokine-mediated antimicrobial immune response of catfish, Clarias gariepinus, as a defence against Aeromonas hydrophila. Fish Shellfish Immunol 7:93–104

    Article  Google Scholar 

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Acknowledgments

Author SSS like to acknowledge Erasmus Mundus-European Union framework MOVER for his post doctoral fellowship. SSG acknowledges DST, Government of India for awarding INSPIRE doctoral fellowship.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India

    Shib Sankar Sen

  2. Department of Biotechnology, Periyar Maniammai University, Thanjavur, Tamil Nadu, 613403, India

    Sib Sankar Giri & V. Sukumaran

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  1. Shib Sankar Sen
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  2. Sib Sankar Giri
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  3. V. Sukumaran
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Correspondence to Sib Sankar Giri.

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Shib Sankar Sen and Sib Sankar Giri have contributed equally in this work.

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Sen, S.S., Giri, S.S. & Sukumaran, V. Immune responses and protection in rohu vaccinated against Aeromonas hydrophila infection. Aquacult Int 22, 1637–1648 (2014). https://doi.org/10.1007/s10499-014-9770-x

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  • DOI: https://doi.org/10.1007/s10499-014-9770-x

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