Skip to main content
SpringerLink
Log in

Morphometric and molecular characterisation of Cyprinus carpio × Carassius auratus hybrids

  • Published:
Aquaculture International Aims and scope Submit manuscript

Abstract

Among members of the Cyprinidae family, hybridisation is more widespread than in any other group of freshwater fish. Therefore, the aim of this work has been to use two different approaches, biometric and genetic, to assess differences between common carp (Cyprinus carpio) × goldfish (Carassius auratus) hybrids and their parents. Twenty-three length measurements of 10 fish from each group have been performed. Statistical analyses of results were conducted using Levene’s, ANOVA, NIR and Kruskal–Wallis tests. Genetic analysis has been conducted by means of amplified fragment length polymorphism (AFLP) technique. The AFLP allowed us to distinguish groups of fish and showed that hybrids had more in common with C. auratus than with C. carpio.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bagley MJ, Anderson SL, May B (2001) Choice of methodology for assessing genetic impacts of environmental stressors: polymorphism and reproducibility of RAPD and AFLP fingerprints. Ecotoxicol 10:239–244

    Article  CAS  Google Scholar 

  • Briolay J, Galtier N, Brito RM, Bouvet Y (1998) Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences. Mol Phylogenet Evol 9:100–108

    Article  CAS  PubMed  Google Scholar 

  • Brylińska M (2000) Ryby słodkowodne Polski. PWN, Warszawa

    Google Scholar 

  • Chizinski CJ, Pope KL, Wilde GR et al (2010) Implications of stunting on morphology of freshwater fishes. J Fish Biol 76(3):564–579

    Article  CAS  PubMed  Google Scholar 

  • Corti M, Thorpe RS, Sola L et al (1988) Multivariate morphometrics in aquaculture: a case study of six stocks of the common carp (Cyprinus carpio) from Italy. Can J Fish Aquat Sci 45:1548–1554

    Article  Google Scholar 

  • Crespin L, Berrebi P, Lebreton JD (2002) Spatially varying natural selection in a fish hybrid zone. J Fish Biol 61:696–711

    Article  Google Scholar 

  • Durand JD, Brianco PG, Laroche J et al (2003) Insight into the origin of endemic Mediteranean ichthyofauna: phylogeography of Chondrostoma genus (Teleostei, Cyprinidae). J Hered 94(4):315–328

    Article  CAS  PubMed  Google Scholar 

  • Hashimoto DT, Mendoça FF, Senhorini JA et al (2010) Identification of hybrids between neotropical fish Leporinus macrocephalus and Leporinus elongates by PCR-RFLP and multiplex-PCR: tools for genetic monitoring in aquaculture. Aquacult 298:346–349

    Article  CAS  Google Scholar 

  • Hubbs CL (1955) Hybridization between fish species in nature. Syst Zool 4:1–20

    Article  Google Scholar 

  • Hulata G (1995) A review of genetic improvement of the common carp (Cyprinus carpio L.) and other cyprinids by crossbreeding, hybridization and selection. Aquacult 129:143–155

    Article  Google Scholar 

  • Hume DJ, Fletcher AR, Morison AK (1983) Interspecific hybridisation between carp (Cyprinus carpio L.) and goldfish (Carassius auratus L.) from Victorian waters. Aust J Mar Freshwat Res 34:915–919

    Article  Google Scholar 

  • Kennedy M, Fitzmaurice P (1973) Occurrence of cyprinid hybrids in Ireland. Ir Nat J 17:349–351

    Google Scholar 

  • Krauss SL (1999) Complete exclusion of nonsires in an analysis of paternity in a natural plant population using amplified fragment length polymorphism (AFLP). Mol Ecol 8:217–226

    Article  CAS  Google Scholar 

  • Kubota H, Watanabe K, Kakehi Y et al (2008) An assessment of genetic diversity in wild and captive populations of endangered Japanese bitterling Tanakia tanago (Cyprinidae) using amplified fragment length polymorphism (AFLP) markers. Fish Sci 74:494–502

    Article  CAS  Google Scholar 

  • Liu ZJ, Karsi A, Li P et al (2003) An AFLP-based genetic linkage map of Channel catfish (Ictalurus punctatus) constructed by using an interspecific hybrid resource family. Genet 165:687–694

    CAS  Google Scholar 

  • Liu LG, Yan JP, Liu SJ et al (2009) Evolutionary analysis of allotetraploid hybrids of red crucian carp x common carp, based on ISSR, AFLP molecular markers and cloning of cyclins genes. Chin Sci Bull 54(16):2849–2861

    Article  CAS  Google Scholar 

  • O’Hanlon PC, Peakall R (2000) A simple method for the detection of size homoplasy among amplified fragment length polymorphism fragments. Mol Ecol 9:815–816

    Article  PubMed  Google Scholar 

  • Olsson J, Svanbäck R, Eklöv P (2007) Effects of resource level and habitat type on behavioral and morphological plasticity in Eurasian perch. Oecol 152:48–56

    Article  Google Scholar 

  • Papa R, Troggio M, Ajmone-Marsan P et al (2005) An improved protocol for the production of AFLP markers in complex genomes by means of capillary electrophoresis. J Anim Breed Genet 122:62–68

    Article  CAS  PubMed  Google Scholar 

  • Pullan S, Smith PJ (1987) Identification of hybrids between koi (Cyprinus carpio) and goldfish (Carassius auratus). New Zeal J Mar Freshwat Res 21:41–46

    Article  Google Scholar 

  • Schlüter PM, Harris SA (2006) Analysis of multilocus fingerprinting data sets containing missing data. Mol Ecol Not 6:569–572

    Article  Google Scholar 

  • Scott WB, Crossman EJ (1973) Freshwater fishes of Canada. J Fish Res Board Can 184:966

    Google Scholar 

  • Scribner KT, Page KS, Bartron ML (2000) Hybridization in freshwater fishes: a review of case studies and cytonuclear methods of biological inference. Rev Fish Biol Fish 10(3):293–323

    Article  Google Scholar 

  • Smith PJ, McVeagh SM (2005) Genetic analyses of carp, goldfish, and carp–goldfish hybrid in New Zealand. Science and technical Publishing Department of Conservation PO Box 10-420, Wellington, New Zealand, pp 5–20

  • StatSoft Inc. (2009) STATISTICA (data analysis software system), version 9.0. www.statsoft.com

  • Toscano BJ, Pulcini D, Hayden B et al (2010) An ecomorphological framework for the coexistence of two cyprinid fish and their hybrids in a novel environment. Biol J Linn Soc 99:768–783

    Article  Google Scholar 

  • Vos P, Hogers R, Bleeker M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acid Res 23:4407–4414

    Article  CAS  PubMed  Google Scholar 

  • Wu C (1990) Retrospects and prospects of fish genetics and breeding research in China. Aquacult 85:61–68

    Article  Google Scholar 

  • Wyatt PMW, Pitts CS, Butlin RK (2006) A molecular approach to detect hybridization between bream Abramis brama, roach Rutlius rutilus and rudd Scardinius erythrophthalmus. J Fish Biol 69:52–71

    Article  CAS  Google Scholar 

  • Yamamoto G, Takada M, Iguchi K et al (2010) Genetic constitution and phylogenetic relationships among Japanese crucian carps (Carassius). Ichthyol Res 57:215–222

    Article  Google Scholar 

  • Zelinskij YuP, Dmitrenko Yu, Pervozvanskiy V (1992) Evaluation of genome interaction from growth characteristics and karyological and morphological characters in triploid hybrids between wild goldfish and carp reared in Karelia. J Ichthyol 32:65–73

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Aquaculture, West Pomeranian University of Technology, Szczecin, Kazimierza Królewicza 4, 71-550, Szczecin, Poland

    Remigiusz Panicz, Paulina Hofsoe, Jacek Sadowski, Bartosz Mysłowski & Mirosław Półgęsek

Authors
  1. Remigiusz Panicz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paulina Hofsoe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jacek Sadowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bartosz Mysłowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mirosław Półgęsek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Remigiusz Panicz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Panicz, R., Hofsoe, P., Sadowski, J. et al. Morphometric and molecular characterisation of Cyprinus carpio × Carassius auratus hybrids. Aquacult Int 21, 751–758 (2013). https://doi.org/10.1007/s10499-012-9577-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10499-012-9577-6

Keywords

Search

Navigation