Skip to main content
SpringerLink
Log in

Chondroptosis: An immunohistochemical study of apoptosis and Golgi complex in chondrocytes from human osteoarthritic cartilage

  • Published:
Apoptosis Aims and scope Submit manuscript

Abstract

The Golgi complex is thought to play an important role in the apoptotic process of osteoarthritic (OA) chondrocytes. However, the exact relationship between modifications of the Golgi complex and apoptosis in human OA cartilage requires to be established. We compared the patterns and immunolabeling intensities for anti-Golgi 58 K protein with apoptosis markers such as TUNEL and caspase-2L in OA cartilage removed from patients during knee total replacement surgery. We observed important modifications in labeling of the Golgi 58 K protein in OA chondrocytes compared with normal cell. Immunohistochemical analysis revealed co-localization between 58 K protein and caspase-2L, suggesting that this enzyme was localized in Golgi complex of OA chondrocytes. In addition, these cells labeled positive with the TUNEL technique, but in different proportions to caspase-2L. Our results support the concept, previously reported, that apoptosis in OA cartilage (chondroptosis) might be a variant of the classical apoptosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Farquhar MG, Palade GE. The Golgi apparatus: 100 years of progress and controversy. Trends Cell Biol 1998; 8: 2–10.

    Article  PubMed  Google Scholar 

  2. Rios RM, Bornens M. The Golgi apparatus at the cell centre. Curr Opin Cell Biol 2003; 15: 60–66.

    Article  PubMed  Google Scholar 

  3. Lane JD, Lucocq J, Pryde J, Barr FA, Woodman PG, Allan VJ, Lowe M: Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis. J Cell Biol 2002; 156: 495–509.

    Article  PubMed  Google Scholar 

  4. Mancini M, Machamer CE, Roy S, Nicholson DW, Thornberry NA, Casciola-Rosen LA. Caspase-2 is localized at the Golgi complex and cleaves golgin-160 during apoptosis. J Cell Biol 2000; 149: 603–612.

    Article  PubMed  Google Scholar 

  5. Kouri JB, Rojas L, Perez E, Abbud-Lozoya KA. Modifications of Golgi complex in chondrocytes from osteoarthrotic (OA) rat cartilage. J Histochem Cytochem 2002; 50: 1333–1340.

    PubMed  Google Scholar 

  6. Kouri JB, Rosales-Encina JL, Chaudhuri PP, Luna J, Mena R. Apoptosis in human osteoarthritic cartilage: A microscopy report. Med Sci Res 1997; 25: 245–248.

    Google Scholar 

  7. Blanco FJ, Ochs RL, Schwarz H, Lotz M. Chondrocyte apoptosis induced by nitric oxide. Am J Pathol 1995; 146: 75–85.

    PubMed  Google Scholar 

  8. Hashimoto S, Ochs RL, Komiya S, Lotz M. Linkage of chondrocyte apoptosis and cartilage degradation in human osteoarthritis. Arthritis Rheum 1998; 41: 1632–1638.

    Article  PubMed  Google Scholar 

  9. Kim HA, Lee YJ, Seong SC, Choe KW, Song YW. Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol 2000; 27: 455–462.

    PubMed  Google Scholar 

  10. Roach HI, Aigner T, Kouri JB. Chondroptosis: A variant of apoptotic cell death in chondrocytes? Apoptosis 2004; 9: 265–277.

    Article  PubMed  Google Scholar 

  11. Kellgren JH, Lawrence JS. Radiological assessment of osteo-arthrosis. Ann Rheum Dis 1957; 16: 494–502.

    PubMed  Google Scholar 

  12. Bennett M, Macdonald K, Chan SW, Luzio JP, Simari R, Weissberg P. Cell surface trafficking of Fas: A rapid mechanism of p53-mediated apoptosis. Science 1998; 282: 290–293.

    Google Scholar 

  13. Zhang XD, Franco AV, Nguyen T, Gray CP, Hersey P. Differential localization and regulation of death and decoy receptors for TNF-related apoptosis-inducing ligand (TRAIL) in human melanoma cells. J Immunol 2000; 164: 3961–3970.

    PubMed  Google Scholar 

  14. Gupta S. Molecular signalling in death receptor and mitochondrial pathways of apoptosis (Review). Int J Oncol 2003; 22: 15–20.

    PubMed  Google Scholar 

  15. Maag RS, Hicks SW, Machamer CE. Death from within: Apoptosis and the secretory pathway. Curr Opin Cell Biol 2003; 15: 456–461.

    Article  PubMed  Google Scholar 

  16. Guo Y, Srinivasula SM, Druilhe A, Fernandes-Alnemri T, Alnemri ES. Caspase-2 induces apoptosis by releasing proapoptotic proteins from mitochondria. J Cell Biol 2002; 159: 739–745.

    Article  PubMed  Google Scholar 

  17. Walker A, Ward C, Sheldrake TA, Dransfield I, Rossai AG, Pryde JG, Haslett C. Golgi fragmentation during Fas-mediated apoptosis is associated with the rapid loss of GM130. Biochem Biophys Res Commun 2004; 316: 6–11.

    Article  PubMed  Google Scholar 

  18. Shikama Y, UM, Miyashita T, Yamada M. Comprehensive studies on sub cellular localizations and cell death-inducing activities of eight GFP-tagged apoptosis-related caspases. Exp Cell Res 2001; 264: 315–325.

  19. Breckenridge DG, Germain M, Mathai JP, Nguyen M, Shore GC. Regulation of apoptosis by endoplasmic reticulum pathways. Oncogene 2003; 22: 8608–8618.

    PubMed  Google Scholar 

  20. Kouri JB, Abbud-Lozoya KA, Rojas-Morales L. Kinetics of the ultrastructural changes in apoptotic chondrocytes from an osteoarthrosis rat model: A window of comparison to the cellular mechanism of apoptosis in human chondrocytes. Ultrastruct Pathol 2002; 26: 33–40.

    PubMed  Google Scholar 

  21. Goggs R, Carter SD, Schulze-Tanzil G, Shakibaei M, Mobasheri A. Apoptosis and the loss of chondrocyte survival signals contribute to articular cartilage degradation in osteoarthritis. Vet J 2003; 166: 140–158.

    Article  PubMed  Google Scholar 

  22. Kim HA, Suh DI, Song YW. Relationship between chondrocyte apoptosis and matrix depletion in human articular cartilage. J Rheumatol 2001; 28: 2038–2045.

    PubMed  Google Scholar 

  23. Hashimoto S, Ochs RL, Rosen F, Quach J, McCabe G, Solan J, Seegmiller JE, Terkeltaub R, Lotz M. Chondrocyte-derived apoptotic bodies and calcification of articular cartilage. Proc Natl Acad Sci USA. 1998; 95: 3094–3099.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Patologìa Experimental, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), México D.F., México

    E. Pérez H, J. Luna M & J. B. Kouri

  2. Unidad de Microscopia Confocal, Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), México D.F., México

    J. Luna M

  3. Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), México D.F., México

    L. Rojas M

  4. Centro de Investigación y de Estudios Avanzados (CINVESTAV-IPN), Ave. Politécnico Nacional 2508, Col. San Pedro Zacatenco, C.P. 07360, México, D.F., México

    J. B. Kouri

Authors
  1. E. Pérez H
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Luna M
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Rojas M
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. B. Kouri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. B. Kouri.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pérez H, E., Luna M, J., Rojas M, L. et al. Chondroptosis: An immunohistochemical study of apoptosis and Golgi complex in chondrocytes from human osteoarthritic cartilage. Apoptosis 10, 1105–1110 (2005). https://doi.org/10.1007/s10495-005-0649-1

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10495-005-0649-1

Keywords

Search

Navigation