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Acaricidal activity and repellency of essential oil from Piper aduncum and its components against Tetranychus urticae

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Abstract

The chemical composition of essential oil of leaves of Piper aduncum L., growing wild in a fragment of the Atlantic Rainforest biome in northeastern Brazil, was determined through gas chromatography-mass spectrometry. The acaricidal activity and repellency of the essential oil and its components [dillapiole (0.28 g/ml), α-humulene (0.016 g/ml), (E)-nerolidol (0.0007 g/ml) and β-caryophyllene (0.0021 g/ml)] were evaluated in the laboratory against adults of Tetranychus urticae Koch. The mites were more susceptible to the oil in fumigation tests (LC50 = 0.01 μl/l of air) than in contact test with closed Petri dish (LC50 = 7.17 μl/ml); mortality was reduced by approximately 50 % in the latter test. The repellent action of the oil and toxicity by fumigation and contact did not differ significantly from the positive control (eugenol). The repellent activity was attributed to the components (E)-nerolidol, α-humulene and β-caryophyllene, whereas toxicity by fumigation and contact was attributed to β-caryophyllene. The effect of Piper oil and the role of its components regarding host plant preference with a two-choice leaf disk test are also discussed.

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References

  • Adams RP (2001) Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy. Allured Publishing Corporation, Carol Stream

    Google Scholar 

  • Almeida RRP, Souto RNP, Bastos CN, Silva MHL, Maia JGS (2009) Chemical variation in Piper aduncum and biological properties of Its dillapiole-rich essential oil. Chem Biodivers 6:1427–1434

    Article  PubMed  Google Scholar 

  • Amin MM, Mizell RF, Flowers RW (2009) Response of the predatory mite Phytoseiulus macropilis (Acari: Phytoseiidae) to pesticides and kairomones of three spider mite species (Acari: Tetranychidae), and non-prey food. Fla Entomol 92:554–562

    Article  CAS  Google Scholar 

  • Attia S, Grissa KL, Lognay G, Heuskin S, Mailleux AC, Hance T (2011) Chemical composition and acaricidal properties of Deverra scoparia essential oil (Araliales: Apiaceae) and blends of its major constituents against Tetranychus urticae (Acari: Tetranychidae). J Econ Entomol 104:1220–1228

    Article  PubMed  CAS  Google Scholar 

  • Costa JGM, Santos PF, Brito SA, Rodrigues FFG, Coutinho HDM, Botelho MA, Lima SG (2010) Composição química e toxicidade de óleos essenciais de espécies de Piper frente as larvas de Aedes aegypti L. (Diptera: Culicidae). Latin Am J Pharm 29:463–467

    Google Scholar 

  • DARP (2012) Database of arthropods resistance to pesticides. http://www.pesticideresistance.org/DB/index.html (05 Jan 2012)

  • Dyer LA, Palmer ADN (2004) Piper: a model genus for studies of phytochemistry, ecology, and evolution. Springer, New York

    Google Scholar 

  • Facundo VA, Polli AR, Rodrigues RV, Militão JSLT, Stabelli RG, Cardoso CT (2008) Constituintes químicos fixos e voláteis dos talos e frutos de Piper tuberculatum Jacq. e das raízes de P. hispidum H. B. K. Acta Amaz 38:733–742

    Article  Google Scholar 

  • Flamini G (2006) Acaricides of natural origin. Part 2. Review of the literature (2002–2006). Nat Prod Commun 1:1151–1158

    CAS  Google Scholar 

  • Guerrini A, Sacchetti G, Rossi D, Paganetto G, Muzzoli M, Andreotti E, Tognolini M, Maldonado ME, Bruni R (2009) Bioactivities of Piper aduncum L. and Piper obliquum Ruiz & Pavon (Piperaceae) essential oils from Eastern Ecuador. Environ Toxicol Pharmacol 27:39–48

    Article  PubMed  CAS  Google Scholar 

  • Guimarães EF, Giordiano LCS (2004) Piperaceae do Nordeste Brasileiro I: Estado do Ceará. Rodriguesia 55:21–25

    Google Scholar 

  • Gupta MP, Arias TD, Smith R (1983) The composition of the essential oil of Piper aduncum L. from Panama. Rev Latinoam Quim 14:35–36

    CAS  Google Scholar 

  • Herron GA, Rophail J (2003) First detection of chlorfenapyr (Secure®) resistance in two-spotted spider mite (Acari:Tetranychidae) from nectarines in an Australia orchard. Exp Appl Acarol 31:131–134

    Article  PubMed  CAS  Google Scholar 

  • IBGE (2010) Instituto Brasileiro de Geografia e Estatística. http://www.ibge.gov.br/home/presidencia/noticias/noticia_visualiza.php?idnoticia=169. Accessed 02 April 2011

  • Isman MB, Miresmailli S, Machial CM (2011) Commercial opportunities for pesticides based on plant essential oils in agriculture, industry and consumer products. Phytochem Rev 10:197–204

    Article  CAS  Google Scholar 

  • Jantan BI, Ahmad AR, Ahmad AS, Ali NA (1994) A comparative study of the essential oils of five Piper species from peninsular Malaysia. Flav Fragr J 9:339–342

    Article  Google Scholar 

  • Leeuwen TV, Vontas J, Tsagkarakou A, Tirry L (2009) Mechanisms of acaricide resistance in the two spotted spider mite Tetranychus urticae. In: Ishaaya I, Horowitz AR (eds) Biorational control of arthropod pests. Springer, New York, pp 347–393

    Chapter  Google Scholar 

  • LeOra Software (1987) POLO-PC: a user’s guide to Probit Logit analysis. Berkely

  • Maia JGS, Zohhbi MGB, Andrade EHA, Santos AS, Silva MHL, Luz AIR, Bastos CN (1998) Constituents of the essential oil of Piper aduncum L. growing wild in the Amazon region. Flav Fragr J 13:269–272

    Article  CAS  Google Scholar 

  • Miresmailli S, Isman MB (2006) Efficacy and persistence of rosemary oil as an acaricide against Twospotted spider mite (Acari: Tetranychidae) on greenhouse tomato. J Econ Entomol 99:2015–2023

    Article  PubMed  Google Scholar 

  • Miresmailli S, Bradbury R, Isman MB (2006) Comparative toxicity of Rosmarinus officinalis L. essential oil and blends of its major constituents against Tetranychus urticae Koch (Acari: Tetranychidae) on two different host plants. Pest Manage Sci 62:366–371

    Article  CAS  Google Scholar 

  • Moraes GJ (2001) Pequeno, mas danoso. Cultivar grandes culturas 28. http://www.grupocultivar.com.br/site/content/artigos/artigos.php?id=564. Accessed 29 July 2011

  • Moraes GJ, Flechtmann CHW (2008) Manual de acarologia: acarologia básica e ácaros de plantas cultivadas no Brasil. Holos, Ribeirão Preto

    Google Scholar 

  • Nerio LS, Olivero-Verbel J, Stashenko E (2009) Repellent activity of essential oils from seven aromatic plants grown in Colombia against Sitophilus zeamais Motschulsky (Coleoptera). J Stored Prod Res 45:212–214

    Article  CAS  Google Scholar 

  • Oliveira JCS, Dias IJM, da Camara CAG (2006) Volatile constituents of the leaf oils of Piper aduncum L. from different regions of Pernambuco (Northeast of Brazil). J Essent Oil Res 18:557–559

    Google Scholar 

  • Pereira ACRL, Oliveira JV, Gondim JMGC, da Camara CAG (2008) Atividade inseticida de óleos essenciais e fixos sobre Callosobruchus maculatus (Fabr., 1775) (Coleoptera: Bruchidae) em grãos de caupi [Vigna unguiculata (L.) WALP.]. Ciênc Agrotec 32:717–724

    Article  CAS  Google Scholar 

  • Pino JA, Marbot R, Bello A, Urquiola A (2004) Essential oils of Piper peltatum (L.) Miq. and Piper aduncum L. from Cuba. J Essent Oil Res 16:124–126

    CAS  Google Scholar 

  • Pohlit AM, Pinto ACS, Mause R (2006) Piper aduncum L.: Planta Pluripotente e Fonte de Substâncias Fitoquímicas Importantes. Rev Fitos 2:7–18

    CAS  Google Scholar 

  • Pontes WJT, de Oliveira JCS, da Camara CAG, Lopes ACHR, Godim-Júnior MGC, de Oliveira JV, Schwartz MOE (2007) Composition and acaricidal activity of the resin’s essential oil of Protium bahianum Daly against two spotted spider mite (Tetranychus urticae). J Essent Oil Res 19:379–383

    CAS  Google Scholar 

  • Robertson JL, Preisler HK (1992) Pesticide bioassays with arthropods. CRC Press, California

    Google Scholar 

  • SAS Institute (2002) SAS user’s guide: statistics, ver. 9.0, 7th edn. SAS Institute, Cary

    Google Scholar 

  • Scott IM, Jensen HR, Philogène BJR, Arnason JT (2008) A review of Piper spp. (Piperaceae) phytochemistry, insecticidal activity and mode of action. Phytochem Rev 7:65–75

    Article  CAS  Google Scholar 

  • Silva WC, Martins JRS, Souza HEM, Heinzen H, Cesio MV, Mato M, Albrecht F, Azevedo JL, Barros NM (2009) Toxicity of Piper aduncum L. (Piperales: Piperaceae) from the Amazon forest for the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Vet Parasitol 164:267–274

    Article  PubMed  Google Scholar 

  • Smith RM, Kassim H (1979) The essential oil of Piper aduncum from Fiji. New Zeal J Sci 22:127–128

    CAS  Google Scholar 

  • Stumpf N, Nauen R (2001) Cross-resistance, inheritance, and biochemistry of mitochondrial electron transport inhibitor-acaricide resistance in Tetranychus urticae (Acari: Tetranychidae). J Econ Entomol 94:1577–1583

    Article  PubMed  CAS  Google Scholar 

  • Van den Dool H, Kratz PH (1963) A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J Chromatogr A 11:463–471

    Article  Google Scholar 

  • Van Leeuwen T, Stillatus V, Tirry L (2004) Genetic analysis and cross-resistance spectrum of a laboratory-selected chlorfenapyr resistant strain of two-spotted spider mite (Acari:Tetranychidae). Exp Appl Acarol 32:249–261

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to the Brazilian fostering agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Proc. No. 476503/2009-4) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE—APQ No. 0520-55.01/08) for grants and financial support.

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Authors and Affiliations

  1. Programa de Pós-graduação em Entomologia Agrícola, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, Sn, 52, Recife, PE, 171-900, Brazil

    Mário J. C. Araújo, Cláudio A. G. Câmara, Flávia S. Born & César A. Badji

  2. Programa de Pós-graduação em Química, Departamento de Ciências Moleculares, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, Sn, 52, Recife, PE, 171-900, Brazil

    Cláudio A. G. Câmara & Marcílio M. Moraes

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  1. Mário J. C. Araújo
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  2. Cláudio A. G. Câmara
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  3. Flávia S. Born
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  4. Marcílio M. Moraes
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  5. César A. Badji
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Correspondence to Cláudio A. G. Câmara.

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Araújo, M.J.C., Câmara, C.A.G., Born, F.S. et al. Acaricidal activity and repellency of essential oil from Piper aduncum and its components against Tetranychus urticae . Exp Appl Acarol 57, 139–155 (2012). https://doi.org/10.1007/s10493-012-9545-x

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  • DOI: https://doi.org/10.1007/s10493-012-9545-x

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