Abstract
Two novel Gram-negative bacterial strains BT190T and BT191 were isolated from soil collected in Uijeongbu city (37°44′55″N, 127°02′20″E), Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT190T and BT191 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia). The level of 16S rRNA gene sequence similarity between the strains BT190T and BT190 was 99.5%. The strains BT190T and BT191 were closely related to Hymenobacter swuensis DY53T (97.0% 16S rRNA gene similarity), Hymenobacter metallilatus 9PBR-2 T (96.8%), Hymenobacter tibetensis XTM003T (96.8%) and Hymenobacter yonginensis HMD1010T (96.6%). The genome size of strain BT190T was 4,859,864 bp. The DNA G+C content of strain BT190T was 55.3 mol%. Bacterial growth was observed at 4–30 °C (optimum 25 °C) and pH 6.0–9.0 (optimum, pH 6.0–7.0) on R2A agar. Colonies of strains BT190T and BT191 were raised, smooth, circular and red-pink colored. The sole respiratory quinone of strain BT190T was MK-7 and the predominant cellular fatty acids were iso-C15:0, C16:1 ω5c, summed feature 3 (C16:1 ω6c / C16:1 ω7c) and summed feature 4 (iso-C17:1 I / anteiso-C17:1 B). The major polar lipids of strain BT190T were aminophospholipid (APL) and phosphatidylethanolamine (PE). Based on the chemotaxonomic, biochemical, and phylogenetic analysis, strains BT190T and BT191 can be suggested as a novel bacterial species within the genus Hymenobacter, for which the name Hymenobacter puniceus sp. nov is proposed. The type strain of Hymenobacter puniceus is BT190T (= KCTC 72342 T = NBRC 114860 T).
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Acknowledgements
We are grateful to Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.
Funding
This work was supported by a research grant from Seoul Women’s University (2021) and by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment of the Republic of Korea (NIBR202002108).
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The 16S rRNA gene sequence of the strain BT190T was deposited in GenBank/EMBL/DDBJ under the accession numbers are MN795741. The draft genome sequence of the strain BT190T is available at the following accession JACSCX000000000.
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10482_2021_1630_MOESM1_ESM.docx
Supplementary file 1 Fig. S1. Transmission electron micrograph of strains BT190T (a) and BT191 (b) from cultures grown on R2A agar for 3 days at 25 °C. Bars are 500 nm (DOCX 568 KB)
10482_2021_1630_MOESM2_ESM.docx
Supplementary file 2 Fig. S2. Genome-based phylogenetic tree reconstructed using a set of 92 UBCGs. The numbers at the nodes indicate the gene support index (maximal value is 92). Bar, 0.02 substitutions per position (DOCX 57 KB)
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Supplementary file 3 Fig. S3. Polar lipid profile of strain BT190T after two-dimensional chromatography. (a) total lipids; (b) phospholipids; (c) aminolipids; (d) glycolipids. Abbreviations: PL, phospholipid; APL, aminophospholipid; PE, phosphatidylethanolamine; GL, glycolipid; L, lipid (DOCX 1167 KB)
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Park, Y., Maeng, S., Damdintogtokh, T. et al. Hymenobacter puniceus sp. nov., radiation resistant bacteria isolated from soil in South Korea. Antonie van Leeuwenhoek 114, 1647–1655 (2021). https://doi.org/10.1007/s10482-021-01630-7
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DOI: https://doi.org/10.1007/s10482-021-01630-7