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Propylenella binzhouense gen. nov., sp. nov. isolated from activated sludge, and proposal of Propylenellaceae fam. nov.

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Abstract

A Gram-stain-negative, non-spore-forming, non-motile, short-rod-shaped, and aerobic bacterial strain (designated L72T) was isolated from propylene oxide saponification wastewater activated sludge obtained from a wastewater treatment facility in Binzhou (Shandong Province, PR China). Strain L72T grew between 25 and 40 °C (optimum growth at 30 °C). The pH range for growth was between 6.0 and 8.0 (optimum growth at pH 7.0). The range of NaCl concentrations for the growth of strain L72T was 0–3.0 % (w/v), with optimum growth at 1.0–2.0 % (w/v). The major cellular fatty acids of strain L72T were C19:0cyclo ω8c, C18:1ω7c, iso-C15:0, and anteiso-C15:0. Strain L72T contained Q–10 as the predominant respiratory quinone. The polar lipid profile was composed of Phosphatidylcholine, Glycolipid, Aminophospholipid, Phosphatidylethanolamine, Phosphatidylserine, Phosphatidyldimethylethanolamine, one unknown lipid (L) and two unidentified Phospholipids (PL). Genome sequencing revealed a genome size of 4,703,686 bp and a G + C content of 69.0 mol%. The 16S rRNA gene sequence similarities of strain L72T with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain L72T formed a distinct phylogenetic lineage within the order Hyphomicrobiales, separating them from members of all families. Strain L72T showed 70.7% average nucleotide identity and 18.6% digital DNA-DNA hybridization identity with the closely related species Rhodoligotrophos defluvii. Based on the phenotypic, phylogenetic and chemotaxonomic data, a new family Propylenellaceae fam. nov. comprising the genus Propylenella gen. nov. and species Propylenella binzhouense sp. nov. is proposed. The type strain is L72T (=  CCTCC AB 2019081T  =  KCTC 72254T).

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References

  • Auling G, Busse H-J, Egli T, El-Banna T, Stackebrandt E (1993a) Description of the Gram-negative, obligately aerobic, nitrilotriacetate (nta)-utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov. Syst Appl Microbiol 16:104–112

    Article  Google Scholar 

  • Auling G, Busse HJ, Egli T, El-Banna T, Stackebrandt E (1993b) Description of the gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov. Syst. Appl Microbiol 16:104–112

    Article  Google Scholar 

  • Bernardet JF, Nakagawa Y, Holmes B (2002) Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070

    CAS  PubMed  Google Scholar 

  • Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868

    Article  CAS  PubMed  Google Scholar 

  • Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al (2018) Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 68:461–466

    Article  CAS  PubMed  Google Scholar 

  • Cowan ST, Steel KJ (1974) Bacterial characters and characterization, 2nd edn. Cambridge University Press, Cambridge

    Google Scholar 

  • Deng SK, Chen GQ, Chen Q, Cai S, Yao L et al (2014) Rhodoligotrophos jinshengii sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 64:3325–3330

    Article  CAS  PubMed  Google Scholar 

  • Doronina NV, Kaparullina EN, Trotsenko YA, Nortemann B, Bucheli-Witschel M et al (2010) Chelativorans multitrophicus gen. nov., sp. nov. and Chelativorans oligotrophicus sp. nov., aerobic EDTA-degrading bacteria. Int J Syst Evol Microbiol 60:1044–1051

    Article  CAS  PubMed  Google Scholar 

  • Doronina NV, Poroshina MN, Kaparullina EN, Ezhov VA, Trotsenko YA (2013) Methyloligella halotolerans gen. nov., sp. nov. and Methyloligella solikamskensis sp. nov., two non-pigmented halotolerant obligately methylotrophic bacteria isolated from the Ural saline environments. Syst Appl Microbiol 36:148–154

    Article  CAS  PubMed  Google Scholar 

  • Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ (2014) Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64:1690–1696

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  CAS  PubMed  Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Fukuda W, Yamada K, Miyoshi Y, Okuno H, Atomi H et al (2012) Rhodoligotrophos appendicifer gen. nov., sp. nov., an appendaged bacterium isolated from a freshwater Antarctic lake. Int J Syst Evol Microbiol 62:1945–1950

    Article  CAS  PubMed  Google Scholar 

  • Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM et al (2020) Analysis of 1000 + type-strain genomes substantially improves taxonomic classification of Alphaproteobacteria. Front Microbiol 11:468

    Article  PubMed  PubMed Central  Google Scholar 

  • Hou L, Zhang Y, Sun J, Xie X (2015) Acuticoccus yangtzensis gen. nov., sp. nov., a novel member in the family Rhodobacteraceae, isolated from the surface water of the Yangtze Estuary. Curr Microbiol 70:176–182

    Article  CAS  PubMed  Google Scholar 

  • Kim D, Kang K, Ahn TY (2017) Chthonobacter albigriseus gen. nov., sp. nov., isolated from grass-field soil. Int J Syst Evol Microbiol 67:883–888

    Article  CAS  PubMed  Google Scholar 

  • Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded Ion exchanger as stationary phases. J Liq Chromatogr 5:2359–2367

    Article  CAS  Google Scholar 

  • Kulichevskaya IS, Danilova OV, Tereshina VM, Kevbrin VV, Dedysh SN (2014) Descriptions of Roseiarcus fermentans gen. nov., sp. nov., a bacteriochlorophyll a-containing fermentative bacterium related phylogenetically to alphaproteobacterial methanotrophs, and of the family Roseiarcaceae fam. nov. Int J Syst Evol Microbiol 64:2558–2565

    Article  CAS  PubMed  Google Scholar 

  • Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ (2014) Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 64:2204–2209

    Article  CAS  PubMed  Google Scholar 

  • Liu YL, Meng D, Li RR et al (2019) Rhodoligotrophos defluvii sp. nov. isolated from activated sludge. Int J Syst Evol Microbiol 69(12):3830–3836

    Article  CAS  PubMed  Google Scholar 

  • Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60–73

    Article  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Mu DS, Liang QY, Wang XM, Lu DC, Shi MJ et al (2018) Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 6:230

    Article  PubMed  PubMed Central  Google Scholar 

  • Patt TE, Cole GC, Hanson RS (1976) Methylobacterium, a new genus of facultatively methylotrophic bacteria. Int J Syst Bacteriol 26:226–229

    Article  CAS  Google Scholar 

  • Qu Z, Jiang F, Chang X, Qiu X, Ren L et al (2014) Psychroglaciecola arctica gen. nov., sp. nov., isolated from Arctic glacial foreland soil. Int J Syst Evol Microbiol 64:1817–1824

    Article  CAS  PubMed  Google Scholar 

  • Richter M, Rosselló-Móra R (2009) Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA 106:19126–19131

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101 Newark, DE: MIDI Inc

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  • Suarez C, Ratering S, Geissler-Plaum R, Schnell S (2014) Hartmannibacter diazotrophicus gen. nov., sp. nov., a phosphate-solubilizing and nitrogen-fixing alphaproteobacterium isolated from the rhizosphere of a natural salt-meadow plant. Int J Syst Evol Microbiol 64:3160–3167

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xie CH, Yokota A (2005) Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa. Int J Syst Evol Microbiol 55:1233–1237

    Article  CAS  PubMed  Google Scholar 

  • Xu XW, Wu YH, Wang CS, Oren A, Wu M et al (2007) Haloferax larsenii sp. nov., an extremely halophilic archaeon from a solar saltern. Int J Syst Evol Microbiol 57:717–720

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang SJ, Im WT, Lee ST, Oh TK (2008) Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus. Int J Syst Evol Microbiol 58:2224–2228

    Article  CAS  PubMed  Google Scholar 

  • Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Mol Biol Evol 67:1613–1617

    CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (32070111, 31870105).

Funding

The GenBank accession number for the 16S rRNA gene sequence of Propylenella binzhouense L72T is MK124547. The whole genome shotgun project of strain L72T has been deposited at DDBJ/ENA/GenBank under the accession number SPKJ00000000. The version described in this paper is version SPKJ01000000.

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Authors and Affiliations

  1. School of Biological Science and Technology, University of Jinan, Jinan, 250022, People’s Republic of China

    Yu-Ling Liu, Dong Meng, Fang Wang, Xiao-Fan Gong, Peng-Fei Gu, Xiang-Yu Fan & Qiang Li

  2. Marine College, Shandong University, Weihai, 264209, People’s Republic of China

    Zong-Jun Du

  3. Shandong Provincial ENT Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250000, People’s Republic of China

    Ji-Dong Zou

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  1. Yu-Ling Liu
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  2. Dong Meng
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  3. Fang Wang
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Contributions

Author contributions LYL and LQ designed research and project outline. LYL, WF, MD, GXF, GPF and FXY performed isolation, deposition, and identification. LYL, WF, DZJ, ZJD and LQ drafted the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Ji-Dong Zou or Qiang Li.

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Liu, YL., Meng, D., Wang, F. et al. Propylenella binzhouense gen. nov., sp. nov. isolated from activated sludge, and proposal of Propylenellaceae fam. nov.. Antonie van Leeuwenhoek 114, 225–233 (2021). https://doi.org/10.1007/s10482-020-01514-2

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