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Spirosoma taeanense sp. nov., a radiation resistant bacterium isolated from a coastal sand dune

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Abstract

An aerobic, Gram-negative, non-motile, non-spore-forming, rod-shaped, and pale yellow-colored bacterial strain, designated TS118T, was isolated from a sand sample obtained from a coastal sand dune after exposure to 3 kGy of gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the genus Spirosoma and most closely related to Spirosoma metallicum PR1014kT (95.1% similarity). The genome of strain TS118T is constituted by one chromosome (5,691,492 bp) and one plasmid (28,440 bp) and has a G + C content of 52.7%. The genome contains 4641 protein coding sequences (CDSs), 38 tRNAs, and 11 rRNAs. The predominant fatty acids of strain TS118T were C16:1 ω5c, iso-C15:0, C16:0, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and iso-C17:0 3-OH. The major polar lipids were phosphatidylethanolamine, an unidentified amino lipid and an unidentified aminophospholipid. The main respiratory quinone was menaquinone-7 (MK-7). The novel strain showed resistance to gamma radiation with a D10 value (i.e., the dose required to reduce the bacterial population by tenfold) of 4.3 kGy. Based on the phylogenetic, physiological, and chemotaxonomic characteristics, strain TS118T represents a novel species, for which the name Spirosoma taeanense sp. nov. is proposed. The type strain is TS118T (=KCTC 72898T =JCM 34024T).

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Availability of data and material

The complete genome sequence of strain TS118T generated during the current study has been deposited in GenBank with the accession codes, https://ncbi.nlm.nih.gov/nuccore/CP053435.1 and https://ncbi.nlm.nih.gov/nuccore/CP053436.1. The 16S rRNA gene sequence also is available in the GenBank repository, https://ncbi.nlm.nih.gov/nuccore/ MN911322.1.

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Funding

This research was supported by the Nuclear R&D program of the Ministry of Science and ICT (MSIT), Republic of Korea.

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Authors and Affiliations

  1. Radiation Research Division, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea

    Ji Hee Lee, Jong-Hyun Jung, Min-Kyu Kim & Sangyong Lim

  2. Department of Radiation Science and Technology, University of Science and Technology, Daejeon, 34113, Republic of Korea

    Jong-Hyun Jung & Sangyong Lim

  3. Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea

    Han Na Choe

  4. Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon, 57922, Republic of Korea

    Chi Nam Seong

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  1. Ji Hee Lee
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  4. Han Na Choe
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  5. Chi Nam Seong
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Contributions

Conceptualization, funding acquisition, and supervision: SL; Resources: M-KK; Investigation validation, and visualization: JHL, J-HJ, HNC; Data curation and formal analysis: JHL, J-HJ, CNS; Writing-original draft: JHL; Writing-review and editing: SL.

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Correspondence to Sangyong Lim.

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Lee, J.H., Jung, JH., Kim, MK. et al. Spirosoma taeanense sp. nov., a radiation resistant bacterium isolated from a coastal sand dune. Antonie van Leeuwenhoek 114, 151–159 (2021). https://doi.org/10.1007/s10482-020-01508-0

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