Abstract
A Gram-positive, rod-shaped, endospore-forming bacterium with multiple flagella, designated XXST-01T, was isolated from deep-sea sediment of Yap Trench with a depth of 6300 m. Activity of oxidase and catalase were found to be positive. Growth was observed at 10–45 °C (optimum 37 °C), pH 6–9 (optimum 7.0), 0–6% NaCl (optimum 0–0.5%, w/v) and 0.1–50 MPa (optimum 0.1 MPa). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XXST-01T belonged to the genus Bacillus. Strain XXST-01T was closely related to Bacillus kyonggiensis NB22T (98.4%), Bacillus siralis 171544T (97.53%), Bacillus massiliosenegalensis JC6T (97.30%), Bacillus oceanisediminis H2T (97.27%), Bacillus mesophilum IITR-54T (97.12%) and Bacillus depressus BZ1T (97.09%). The ANI and the DNA–DNA hybridization estimate values between strain XXST-01T and closely related type strains were 70.91–90.15% and 19.80–40.50%, respectively. The principal fatty acids were iso-C15:0, anteiso-C15:0, iso-C16:0, and iso-C14:0. The G+C content of the chromosomal DNA was 38.2 mol%. The respiratory quinone was determined to be MK-7. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and four unidentified aminophospholipids. The combined genotypic and phenotypic data show that strain XXST-01T represents a novel species within the genus Bacillus, for which the name Bacillus yapensis sp. nov. is proposed, with the type strain XXST-01T (=MCCC 1A14143T = JCM 33181T).
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Abbreviations
- MCCC:
-
Marine Culture Collection of China
- JCM:
-
Japan Collection of Microorganisms
- DSM:
-
Deutsche Sammlung von Mikroorganismen und Zellkulturen (German Collection of Microorganisms and Cell Cultures)
- CGMCC:
-
China General Microbiological Culture Collection Center
- ANI:
-
Average nucleotide identity
- DDH:
-
DNA–DNA hybridization
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Acknowledgements
This work was supported by the National Basic Research Program of China (973 Program) (No. 2015CB755901), Fujian Key Science and Technology Program (No. 2018N0017), Xiamen Ocean Economic Innovation and Development Demonstration Project (No. 16PZP001SF16), Scientific Research Foundation of Third Institute of Oceanography, SOA. (2017002), Xiamen Science and Technology Program (3502Z20182029).
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Xu, X., Yu, L., Xu, G. et al. Bacillus yapensis sp. nov., a novel piezotolerant bacterium isolated from deep-sea sediment of the Yap Trench, Pacific Ocean. Antonie van Leeuwenhoek 113, 389–396 (2020). https://doi.org/10.1007/s10482-019-01348-7
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DOI: https://doi.org/10.1007/s10482-019-01348-7