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Pedobacter changchengzhani sp. nov., isolated from soil of Antarctica

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Abstract

A Gram-negative, aerobic, non-motile, pink and rod-shaped bacterium, designated E01020T, was isolated from soil collected from the Chinese Great Wall Station, Antarctica. Comparative analysis of 16S rRNA gene sequences revealed that strain E01020T is a member of the genus Pedobacter, related to Pedobacter alluvionis DSM 19624T (96.8% similarity), Pedobacter agri JCM 15120T (96.5% similarity) and Pedobacter chinensis JDX94T (96.3% similarity). The dDDH values and ANI values of strain E01020T with closely related strains indicate that it can be distinguished from them as a novel species. The DNA G+C content was determined to be 35.2 mol%. The growth of strain E01020T was observed at 4–25 °C (optimal 20 °C), in the presence of 0–1% NaCl (w/v, optimal 0%) and at pH 6.0–8.0 (optimal pH 7.0). Strain E01020T was found to contained menaquinon-7 (MK-7) as only respiratory quinone, iso-C15:0, iso-C17:0 3-OH and Summed feature 3 (C16:1ω6c and/or C16:1ω7c) as major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified lipid and two unidentified aminolipids. On the basis of the results of the phylogenetic and phenotypic analyses, it was suggested that strain E01020T represents a novel species of the genus Pedobacter, for which the name Pedobacter changchengzhani sp. nov. is proposed. The type strain is E01020T (= KCTC 62990T = MCCC 1H00357T).

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Funding

This work was supported by the funding from National Key R&D Program of China (2018YFC1406903), Science and Technology Basic Resources Investigation Program of China (2017FY100300) and the SOA Key Laboratory for Polar Science, Polar Research Institute of China (KP201705).

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Authors and Affiliations

  1. College of Marine Science, Shandong University, Weihai, 264209, China

    Rui-Han He, Zhen-Wei Liu & Zong-Jun Du

  2. Polar Research Institute of China, Ministry of Natural Resources, Shanghai, 200136, China

    Rui-Han He, Zhen-Wei Liu, Yong Yu, Hui-Rong Li & Zong-Jun Du

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  1. Rui-Han He
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  2. Zhen-Wei Liu
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  4. Hui-Rong Li
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Correspondence to Hui-Rong Li or Zong-Jun Du.

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He, RH., Liu, ZW., Yu, Y. et al. Pedobacter changchengzhani sp. nov., isolated from soil of Antarctica. Antonie van Leeuwenhoek 112, 1747–1754 (2019). https://doi.org/10.1007/s10482-019-01305-4

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